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KEMAMPUAN LEMNA (LEMNA PERPUSILLA TORR.) SEBAGAI FITOREMEDIATOR UNTUK MENYERAP LIMBAH NITROGEN DALAM BUDIDAYA IKAN LELE (CLARIAS GARIEPINUS) DI SISTEM RESIRKULASI Amalia, Febrina; Nirmala, Kukuh; Harris, Enang; Widiyanto, Tri
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Penelitian ini bertujuan untuk mengkaji kemampuan lemna (Lemna perpusilla Torr.) sebagai fitoremediator dalam menyerap limbah nitrogen dalam budidaya ikan lele (Clarias gariepinus). Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 3 ulangan. Perlakuan berupa luas tutupan lemna sebesar 14,7%, 29,4%, dan 44,1% dari kolam filter. Padat tebar ikan lele adalah 200 ekor m-3 dengan bobot rata-rata awal 9,67±1,01 g. Hasil penelitian setelah 30 hari menunjukkan bahwa perlakuan luas tutupan lemna 44,1% dapat menyerap limbah N sebesar 4,48±0,04 g N, sedangkan untuk perlakuan luas tutupan lemna 29,4% dan 14,7% dapat menyerap limbah N masing-masing sebesar 4,03±0,02 g N dan 3,50±0,07 g N. Jumlah N dalam biomassa lemna tertinggi juga dicapai oleh perlakuan luas tutupan lemna 44,1% sebesar 28,13±0,74 g N. Sintasan tertinggi 76,33±4,04% juga diperoleh pada perlakuan luas tutupan 44,1%.
PEMANFAATAN Lemna perpusilla SEBAGAI PAKAN KOMBINASI UNTUK IKAN NILA (Oreochromis niloticus) PADA SISTEM RESIRKULASI Ilyas, Anita Prihatini; Nirmala, Kukuh; Harris, Enang; Widiyanto, Tri
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Lemna perpusilla adalah suatu makrofit yang hidup terapung di air, terdapat di seluruh dunia dan banyak ditemukan di air tawar yang kaya nutrien. Tumbuhan ini lebih dikenal sebagai gulma yang cenderung sulit untuk dikendalikan karena memiliki produktivitas yang sangat tinggi. Penelitian untuk menganalisis kemampuan ikan nila (Oreochromis niloticus) dalam memanfaatkan L. perpusilla sebagai pakan kombinasi telah dilakukan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan empat taraf perlakuan dan tiga ulangan. Perlakuan konsentrasi pakan 100% L. perpusilla + 0% pelet, 25% L. perpusilla + 75% pelet, 50% L. perpusilla + 50% pelet, 0% L. perpusilla + 100% pelet. Ikan yang digunakan dalam penelitian ini adalah ikan nila (O. niloticus). Padat tebar ikan 20 individu per waring dengan bobot rata-rata 20±0,01 g per individu. Ikan diberi pakan sebanyak dua kali per hari selama 50 hari. Setiap tujuh hari sekali dilakukan penimbangan bobot tubuh ikan nila. Hasil penelitian menunjukkan bahwa L. perpusilla dapat menggantikan pelet sebagai pakan sebesar 25%. Lemna tidak dapat menggantikan pakan secara keseluruhan karena terkait dengan tingginya serat yang terkandung di dalamnya yang dapat mempersingkat waktu tubuh untuk melakukan proses pencernaan dan penyerapan nutrisi.
The Role of Bakau Snail, Telescopium telescopium L., as Biofilter in Waste Water Management of Intensive Shrimp Culture , Hamsiah; Daniel Djokosetiyanto; E. M. Adiwilaga; Kukuh Nirmala
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.587 KB) | DOI: 10.19027/jai.1.57-64

Abstract

ABSTRACTThe objective the experiment is to know the role of bakau snail, Telescopium telescopium L., as biofilter for improving waste water quality in shrimp culture.  The experiment was carried out at laboratory scale.  The parameters that observed in this experiment are physical, chemical and biological of waste water.  Growth and survival rate of snail were also observed. Waste water quality measurement was carried out during a week, while the growth and survival rate were measured during two months.  The aquarium of 30x40x40 cm were filled with 30 l of waste water from intensive shrimp culture.  Bakau snail were stocked to the aquarium with density of 0 (control), 6, 9 and 12 snail/aquarium, and these treatment were replicated 3 times.  The result shown that total organic matter (TOM), total ammonia, dissolved oxygen (DO) of waste water, and growth and survival rate of snail were not different between treatment of stocking density, while the biological oxygen demand (BOD), total suspended solid (TSS), nitrite and nitrate were significantly different (pKey words :  Bakau snail, Telescopium telescopium L.,  biofilter, shrimp culture waste water. ABSTRAKPercobaan ini bertujuan untuk mengkaji peranan keong bakau, Telescopium telescopium L., sebagai biofilter terhadap perbaikan mutu air limbah budidaya tambak udang intesif.  Pertumbuhan dan kelangsungan hidup keong bakau juga dikaji.  Percobaan dilakukan dalam skala laboratorium.  Pengamatan kualitas air fisika, kimia dan biologi air limbah budidaya tambak dilakukan selama seminggu, sedangkan pertumbuhan keong dilakukan selama 2 bulan.  Wadah percobaan yang digunakan adalah akuarium ukuran 30x40x40 cm dan diisi air sebanyak 30 liter yang berasal dari buangan budidaya udang intensif di tambak.  Perlakuan percoban berupa padat tebar keong bakau dalam akuarium yaitu: 0 (tanpa keong), 6, 9 dan 13 ekor/akuarium, dan setiap perlakuan diulang 3 kali. Hasil percobaan menunjukkan bahwa respon peubah kadar bahan organik total (TOM), amoniak total, oksigen terlarut (DO) dalam air limbah, serta pertumbuhan dan kelangsungan hidup keong bakau tidak berbeda antar perlakuan kepadatan, sedangkan BOD5, padatan tersuspensi total (TSS), nitrit dan nitrat berbeda nyata (pKata kunci :  Keong bakau, Telescopium telescopium L., biofilter, air limbah budidaya udang.
Productivity and Chemical Parameters in the Bottom Soil of 1 and 3 years operated pond of Black Tiger Prawn Penaeus monodon Fab. Culture Kukuh Nirmala; E. Yuniar; T. Budiardi
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.721 KB) | DOI: 10.19027/jai.4.5-11

Abstract

This study was carried out to observe the productivity and bottom soil chemical aspects of 1 year  and 3 years operated pond of black tiger prawn Penaeus monodon Fab. culture. The results of study depicted that pond operated for 3 years contained higher (P
Effect of Hardness in 3 ppt of Water Salinity on growth and survival rates of Barb Fish (Barbus conhonius Hamilton-Buchanan) Kukuh Nirmala; R. Wulandari; D. Djokosetiyanto
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.63 KB) | DOI: 10.19027/jai.4.17-24

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This study was conducted to determine the effect of hardness on growth and survival rate of barb fish (Barbus conchonius Hamilton-Buchanan) reared in the 3 ppt of water salinity.  Fish were reared in three different hardnesses, i.e., 54, 72, and 90 mg/L CaCO3 for 30 days.  The results of study showed that fish reared in water hardness of 72 mg/L CaCO3 had higher daily growth rate (1.15%) compared with other treatments.  Daily growth rate of fish reared in water hardness of 54 and 90 mg/L CaCO3 were 0.76 and 0.37%, respectively.  There was no effect of hardness on survival rate of barb fish. Keywords: barb fish, Barbus conchonius,  hardness, salinity   ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh kesadahan yang berbeda terhadap laju pertumbuhan dan tingkat kelangsungan hidup ikan barbir (Barbus conchonius Hamilton-Buchanan) yang dipelihara pada air bersalinitas 3 ppt.  Ikan dipelihara dalam 3 tingkat kesadahan yang berbeda, yaitu 54 mg/L CaCO3, 72 mg/L CaCO3, dan 90 mg/L CaCO3 selama 30 hari.  Hasil penelitian menunjukkan bahwa laju perumbuhan harian tertinggi diperoleh pada ikan yang dipelihara pada kesadahan 72 mg/L CaCO3 (1,15%) dibandingkan dengan perlakuan lainnya. Laju pertumbuhan harian ikan yang dipelihara pada kesadahan 54 dan  90 mg/L CaCO3 masing-masing mencapai 0,76% dan 0,37%.  Kelangsungan hidup ikan barb tidak dipengaruhi oleh kesadahan. Kata kunci: ikan barbir, Barbus conchonius, kesadahan, salinitas
The growth performance of Osphronemus goramy reared in saline water with electrical field exposure Kukuh Nirmala; . Rasmawan
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.392 KB) | DOI: 10.19027/jai.9.46-55

Abstract

The aim of this study was to know the optimal salinity level on growth rates of giant gouramy Osphronemus goramy  reared in different salinity with electrical field exposure. Four different salinities tested were 0; 3; 6 and 9‰ with the electrical field exposure of 10 Volt. The experiment design was arranged in completely randoumizes design with four treatments and three replications. Stock density was 3 fish/l with mean initial total body length of 7.18±0.30 cm and initial body weight of 5.68±0.67 g. Result of study showed that the treatment of 3‰ shows the best growth performance with specific growth rates of 1.02±0.10% and growth of absolute length of 0.56±0.18 cm. Key words: Salinity, electrical field, growth rate, Osphronemus goramy   ABSTRAK Tujuan penelitian ini adalah untuk mengetahui salinitas optimal untuk pertumbuhan ikan gurame Osphronemus goramy yang dipelihara pada media bersalinitas berbeda dengan paparan medan listrik. Perlakuan meliputi empat salinitas media yang berbeda: 0, 3, 6, dan 9‰ dengan paparan medan listrik 10 Volt. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan dan 3 ulangan. Padat penebaran ikan adalah 3 ekor/l dengan rata-rata panjang total 7,18±0,30 cm dan bobot rata-rata awal 5,68±0,67 g. Hasil penelitian menunjukkan bahwa pertumbuhan ikan gurame terbaik pada penelitian ini dicapai pada salinitas 3‰ dengan pertumbuhan bobot 1,02±0,10% dan pertumbuhan panjang mutlak 0,56±0,18 cm.   Kata-kata kunci: Salinitas, medan listrik, laju pertumbuhan, Osphronemus goramy
Toxicity of mercury (Hg) on survival and growth rate, hemato- and histopathological parameters ofOreochromis niloticus Kukuh Nirmala; Yuni Puji Hastuti; Vika Yuniar
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1170.716 KB) | DOI: 10.19027/jai.11.38-48

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Heavy metals are serious pollutants of the aquatic environment because of their environmental persistence and ability to be accumulated by aquatic organisms. Oreochromis niloticus exposed to 0, 0.16, 0.5, and 1.0 ppm Hg for 30 days. The aim of this study was to determine the influence of mercury in water on survival rate, growth rates, hematological, and histological parameters of Oreochromis niloticus. This study was conducted from Mei to June 2009. The experimental design was arranged in completely randomized design with four treatments and three replications. Stock density was 8 fish/aquarium with mean initial body weight was 15.70±1.13 g. Growth and survival rates of test fish were decreased with increasing the Hg concentration. Red blood cell (RBC) count, haematocrit content, and haemoglobin content decreased when compared to the control. The number of white blood cells (WBC) increased in mercuric treated fish. The results are statistically significant at p<0.05 level. Keywords:mercury, survival and growth rate, hematology, histopathology, Oreochromis niloticus
Nitrogen and phosphorus absorption capability in environmental culture by Taiwan gravestone Anadonta woodiana Lea Yuni Puji Hastuti; Kukuh Nirmala; Tyas Setioaji
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1165.056 KB) | DOI: 10.19027/jai.11.86-95

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Nitrogen and phosphorus in a water culture are derived from the metabolism of the farmed organism, feed residue and microbial activity in the water. One way of controlling water quality due to waste in the form of nitrogen and phosphorus from aquaculture is to utilize filter feeder organisms of Anadonta woodiana Lea. This study aimed to analysis the effectiveness of the absorption of nitrogen and phosphorus in waters by A. woodiana as filter feeder. A total of 0 (control), five, ten, and 15 gravestone were putted into a tank of 35 L of water derived from Cirata reservoir. Each treatment was given three times repetition. Measurement of total nitrogen and phosphorus in gravestones were performed at baseline and end of treatment. In addition, measurement of total nitrogen and phosphorus in the water tank was measured every ten days. Another measurement is the length and weight of gravestone. Direct observations made on gravestones barnacle survival, pH, dissolved oxygen, temperature, and turbidity every day. Aeration was provided in each aquarium. Based on the results obtained, the survival of gravestone in the density of five gravestones was 93.33%, in ten gravestones density was 90%, while in 15 gravestones was 95%. The percentage reduction in total nitrogen in the water at the end of treatment was 57% for the five gravestones density, and for ten gravestones was 88.2%. The decrease of total phosphorus in water in ten gravestones density was 37.4%, whereas the increase in density of 15 gravestones and total phosphorus water percentage was 26.9%. On the density of ten gravestones, the body weight decreased until to 3.3%, while in the density of 15 gravestones decreased until to 2.5% on day 30. Results of water quality analysis showed that the DO values decreased from baseline to end of treatment (from 5.08 to 4.86 mg/L), the average water temperature from 26.5 to 27.4 °C, pH values 7‒8, and highest turbidity values was obtained in the density of five gravestone. Decrease in the percentage of total nitrogen in the water suggested nitrogen and phosphorus have been absorbted for barnacle growth. Keywords: total phosphorus, total nitrogen,waste,Anadonta woodiana Lea,cultivation
The addition of salt in the water media containing zeolite and active charcoal on closed system transportation of gourami fish fry Osphronemus goramy Lac. Kukuh Nirmala; Yani Hadiroseyani; Riza Purbo Widiasto
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1321.792 KB) | DOI: 10.19027/jai.11.190-201

Abstract

Transportation of fish fry with high density in closed system will reduce levels of O2, increasing CO2 and NH3, will also elevate the fish stress so that increase fish mortality. To reduce the effects of increased CO2 and NH3 can be applied by using zeolite and activated charcoal, while to reduce the fish stress is through the addition of salt. This study aims to determine the dose of salt added into the water containing zeolite and activated charcoal in a closed transportation system with a high fry density for 72 hours. The study was conducted two stages, namely the preliminary study and the primary study. The preliminary study involved the observation of the survival rate of fish fry during fasting, oxygen consumption rate of fish fry, the rate of total ammonia nitrogen (TAN) excretion of fish fry, and the adsorption capacity of TAN by zeolite and activated charcoal. In the primary study, fry transport simulations was carried out for 72 hours in the laboratory. Gourami fry (body length of 4 cm and body weight of 1.7 g) with the fry density of 50 fish/L were placed in the packing bag which has been filled with zeolite as much as 20 g/L and activated charcoal as much as 10 g/L. The study used a completely randomized design with five treatments and two replications: A: blank (without zeolite, activated charcoal, and salt), B: control (20 g/L zeolite+10 g/L activated charcoal), C: 20 g/L zeolite+10 g/L activated charcoal and 1 g/L salt, D: 20 g/L zeolite+10 g/L activated charcoal and 3 g/L of salt, and E: 20 g/ L zeolite+10 g/L activated charcoal and 5 g/L salt. The results of preliminary study showed that the survival rate of fish fry was 100% and active swimming for five days without food, the level of oxygen consumption as much as 1340.28 mgO2, produce NH3 as much as 22.64 mg/L, while zeolite and activated charcoal adsorbs >50% of TAN in time of 120 seconds. In the primary study, the survival rate of fish fry during the 72-hour transportation for treatments A, B, C, D, and E, were 0%, 57%, 59%, 65% and 74%, respectively. Treatment E with survival rate of 74% was obtained at NH3 concentration of 0.016±0.069 mg/L and CO2 as high as 89.89 mg/L while the DO of 4.26 mg/L. The highest growth and survival rate of fry for 17 days post-transportation was treatment E, accounting to 2.95% and 100%, respectively.Keywords: zeolite, active charcoal, closed system transportation, survival rate, gourami fish
Growth performance of pearl goldfish juvenile carassius auratus cultured in 3 ppt salinity with different exposure time of electric field Kukuh Nirmala; Rizky Armansyah; Agus Priyadi
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.447 KB) | DOI: 10.19027/jai.10.165-173

Abstract

ABSTRACTGrowth rate of pearl goldfish juvenile Carassius auratus relatively slow to reach market size which will take approximately three months. To accelerate its growth can be done by providing exposure of the fish to low-power electric fields (10 V) via 3 ppt salinity water, with the goal of providing the close isoosmotic conditions, and also to streamline the flow of electricity from the electrodes to the body of the fish. This study aims to calculate the survival and growth rate of pearl goldfish juvenile of S sizes (2‒4 cm of body length) which were maintained at 3 ppt salinity water and treated by different exposure time of electric field (zero, two, four, and six minutes before feeding) with 10 volt electric power. Fish were cultured at a density of 2 fish/L in the (20×30×20 cm3) aquaria in volume of 6 L of water. Test fish had an average body length of 4.11±0.05 cm and the average body weight of 2.89±0.05 g. Exposure time of electric field were zero, two, four, and six minutes before the fish are fed, performed every day as much as three times i.e. morning, afternoon, and evening. The research design used was completely randomized design with four treatments, namely 0, 2, 4, and 6 (time for exposure is zero/control, two, four, and six minute) with three replications. The results show test fishes exposed to 10 volt electrical field for zero, two, four, and six minutes, have no significant effect on survival rate (P>0.05). For growth performance, four minute exposure treatment gives the best results compared to controls (P<0.05), supported by an increase in the percentage of the ratio of gut length to body length of the fish and higher feed efficiency.Keywords: long exposure to the electric field, growth performance, pearl goldfishABSTRAKPertumbuhan benih ikan hias maskoki mutiara Carassius auratus relatif lambat, karena untuk mencapai ukuran jual memerlukan waktu sekitar tiga bulan. Untuk mempercepat pertumbuhannya dapat dilakukan dengan pemberian paparan medan listrik berdaya rendah (10 V) ke air media budidaya yang dinaikkan salinitasnya menjadi 3 ppt, dengan tujuan memberikan kondisi mendekati isoosmotik, dan juga untuk mengefektifkan arus listrik dari elektroda ke tubuh ikan. Penelitian ini bertujuan untuk menghitung tingkat kelangsungan hidup dan pertumbuhan benih ikan maskoki mutiara ukuran S (2‒4 cm) yang dipelihara pada media bersalinitas 3 ppt yang diberi perlakuan lama waktu pemaparan medan listrik (nol, dua, empat, dan enam menit sebelum ikan diberi pakan) dengan daya 10 volt. Ikan dipelihara dengan kepadatan 2 ekor/L dalam akuarium berukuran 20×30×20 cm3 dengan volume air 6 L. Ikan uji yang digunakan memiliki panjang rata-rata 4,11±0,05 cm dan bobot rata-rata 2,89±0,05 g/ekor. Pemberian paparan medan listrik dilakukan selama nol, dua, empat, dan enam menit sebelum ikan diberi pakan, dilakukan setiap hari sebanyak tiga kali yaitu pagi, siang, dan sore hari. Rancangan penelitian terdiri atas empat perlakuan, yaitu 0, 2, 4, dan 6 (lama paparan nol/kontrol, dua, empat, dan enam menit) yang diulang masing-masing tiga kali ulangan. Hasil pemaparan arus listrik 10 volt selama nol, dua, empat, dan enam menit, tidak memberikan pengaruh yang nyata pada kelangsungan hidup (p>0,05). Untuk kinerja pertumbuhan, perlakuan paparan empat menit memberikan hasil yang terbaik dibandingkan kontrol (p<0,05), didukung oleh peningkatan persentase nilai rasio panjang usus terhadap panjang tubuh ikan serta nilai efisiensi pakan yang lebih tinggi.Kata kunci: lama paparan medan listrik, kinerja pertumbuhan, ikan maskoki mutiara
Co-Authors , Hamsiah , Purnamawati , Robin . Alimuddin . Humairani . Rasmawan . Wiyoto Agus Priyadi Ahmad Habibie Alexander Burhani Marda Alimuddin Alimuddin Anang Hari Kristanto Ani Widiyati Ani Widiyati Ani Widiyati Ani Widiyati Aras, Annisa Khairani Arfan Afandi Arif Faisal Siburian Aris Sando Hamzah Arlina Ratnasari Atma Jaya Salman Muin Bambang Kusmayadi Gunawan Berlianti . Bianingrum Bianingrum Cecep Kusmana Chandra Yudistira D. Djokosetiyanto D. Djokosetyanto Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEDI JUSADI Dewi Puspaningsih Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Islama Dodi Hermawan E. M. Adiwilaga E. Yuniar Eddy Supriyono Enang Haris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Erlania Erlania Erna Yuniarsih Ernik Yuliana Farah Diana farida farida Farida Farida Febrina Amalia Fernando Jongguran Simanjuntak Hanif Azhara, Muhammad Haris luthfi Harton Arfah Hastiadi Hasan I Nyoman Radiarta Idil Ardi Ilyas, Anita Prihatini Iman Rusmana Imron Imron Imron Imron Irzal Effendi Izhar Amirul Haq Jannesa Nasmi John Harianto Hutapea John Harianto Hutapea Joni Haryadi Julie Ekasari Kukuh Adiyana Kukuh Adiyana Kurnia Faturochman Kurnia Faturrohman Lilis Nurjanah M. Faisol Riza Ghozali M. Zairin Junior Mia Setiawati Mia Setiawati Mochamad Nurdin Moh. Burhanuddin Mahmud Muh Azril Muhamad Yamin Muhammad Amien H Muhammad Firmawan Muhammad Zairin Jr. Novi Susianti Nur Bambang Priyo Utomo Nur Fauziyah Nurhidayat Nurhidayat Nurul Taufiqu Rochman Nurul Taufiqu Taufiqu Rochman Prama, Ega Aditya Priyo Handoyo Wicaksono Qorie Astria R. Wulandari Rahmadi Azis Retnosari Retnosari Riani Rahmawati RIDWAN AFFANDI Ridwan Affandi Ridwan Affandi Ridwan Affandi Rina Hesti Utami Riri Ezraneti Ris Dewi Novita Riza Purbo Widiasto Rizky Armansyah Robin Robin Ruspindo Syahputra Saputra, Henry Kasmanhadi Setyo Pertiwi Sheny Permatasari Siska Tridesianti Sri Nuryati Sri Wahyuni Firman Sudarto Sudarto Sugeng Budiharsono Suhaiba Djai Suharyanto Suharyanto Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sulistia Wardani Syarif Budiman T. Budiardi Tatag Budiardi Tri Heru Prihadi Tri Heru Prihadi Tri Widiyanto Triheru Prihadi Tyas Setioaji Vika Yuniar Wahyu Budi Kuntari Wahyu Wahyu Widiatmaka . Widiatmaka widiatmaka Wijianto Wijianto Wijianto Wijianto Wildan Nurusallam Wildan Nurusallam Wildan Nurusallam Wildan Nurussalam Wildan Nurussalam, Wildan Wisriati Lasima Wiwin Ambarwulan Y. Hadiroseyani Yogi Himawan yogi Himawan Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yuli Siti Fatma Yuli Siti Fatma Yuliana Asri Yuliana Asri Yuni Puji Hastuti