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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Primatologi Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak dan Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters Jurnal Kedokteran Hewan
Srihadi Agungpriyono
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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PF-17 The Development of Crude Testicular Cells in In Vitro Culture Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Spermatogenesis is a continuous process in which spermatogonial stem cells (SSC) develop into specific germ cells before terminally differentiating to form spermatozoa.  The process is supported by Sertoli cells, which are in close contact with germ cells in the seminiferous tubules. Sertoli cells provide essential hormonal signals, nutrients, and physical support to germ cells for successful spermatogenesis.The crude testicular cells (CTC) contains many cell types, like Sertoli cell, Leydig cell, spermatogonial stem cell (SSC), spermatocyte and other testicular somatic cells (Shah et all. 2016). Testicular cells are believed to secrete various growth factors that induced the spermatogenesis process.  The spermatogonial stem cells are unique population of cells in the male testis, which dual function.  First self-renewing their population to maintain the number of stem cells, secondary function is differentiating into spermatids in testis (Wang et al.  2015).Spermatogenic cells differentiation  needed the similar microenvironment in vivo spermatogenesis.  The essential nutrients was collected from healty culture and the culture contained mixed population of cells both the somatic cells and spermatogenic cells.  To identification the spermatogenic cells using Periodic Acid Schifft (PAS) staining (Chang et al. 2011). The present study examined the development of crude testicular cells using PAS staining.
Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells I Ketut Mudite Adnyane; Savitri Novelina; Dwi Kesuma Sari; Tutik Wresdiyati; Srihadi Agungpriyono
Media Veteriner Vol. 8 No. 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
Morphology Of The Gut Endocrine Cells In The Gastrointestinal Tract Of The Lesser Mouse Deer (Tragulus javanicus) Srihadi Agungpriyono
Media Veteriner Vol. 4 No. 1 (1997): Media Veteriner
Publisher : Media Veteriner

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Abstract

The ultrastructure of endocrine cells in the gastrointestinal tract of the lesser mouse deer (Tragulus javanicus), the smallest ruminant, was studied using electron microscopy. The cells possess components such as rough endoplasmic reticulum, golgi complexes and mitochondria and characterized prominently by the presence of cytoplasmic secretary granules. The secretary granules were polymorphous, rounded, oval or spindle shaped and varied greatly in size and electron density from one cell type to another. The granules were generally concentrated in the intranuclear region of the cells. Two types of endocrine cells could be observed. Open type cells were oval, triangular or spindle in shape, showed apical lurninal contact by means of microvilli. Closed type cells were generally round or triangular in shape. The endocrine cells were located in the basal portions, close to either capillaries or submucosal nerve fibers. The morphology of the endocrine cells was discussed in relation to their possible functions.
The morphology and the distribution of gut endocrine cells in the gastrointestinal tract of Indonesian native chicken were studied using Grimelius staining method. The endocrine cells were polymorph, round oval, triangular or pyramidal in shapes and scattered among the cells in the mucosal epitheli-um and glands of all portions of the gastrointestinal tract from stomach to rectum. These cells were characterized by the presence of basally located cytoplasmic granules that react positively with th Aryani Sismin Sastyaningtijas; Savitri Novelina; Srihadi Agungpriyono
Media Veteriner Vol. 6 No. 4 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The morphology and the distribution of gut endocrine cells in the gastrointestinal tract of Indonesian native chicken were studied using Grimelius staining method. The endocrine cells were polymorph, round oval, triangular or pyramidal in shapes and scattered among the cells in the mucosal epitheli-um and glands of all portions of the gastrointestinal tract from stomach to rectum. These cells were characterized by the presence of basally located cytoplasmic granules that react positively with the silver granules of the staining method. Two types of endocrine cells were observed in this stu-dy included open type and closed type. In the open type cells cytoplasmic elongation reached the intestinal or glandular lumen. Closed type cells possessed no such elongation but there was cytoplasmic processes run in the basal membrane. Open cells were largely distributed in the intestine while closed type cells with basally cytoplasmic processes were numerous in the gizzard. In general the endocrine cells were numerous in the jejunum of the small intestine. In the distal portion of large intestine we found clusters of endo-crine cells in the glands. The morphology and distribution pattern observed was discussed in relation with their possible functional implications.
Three dimensional architecture of the sub epithelial connective tissue in the forestomach of the lesser mouse deer was studied by scanning electron microscopy after macerated with 10% NaOH. In general, the architecture of the connective tissue in the rumen and reticulum showed similar pattern, which was honeycomb like pattern. This pattern was observed in all portions of the forestomach. Primary wall bordered each cell of the honeycomb. Inside the cells there were some secondary or tertiary wall Savitri Novelina; Srihadi Agungpriyono; Yoshio Yamamoto; Chairun Nisa; Nabuo Kitamura; Junzo Yamada
Media Veteriner Vol. 6 No. 4 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

Three dimensional architecture of the sub epithelial connective tissue in the forestomach of the lesser mouse deer was studied by scanning electron microscopy after macerated with 10% NaOH. In general, the architecture of the connective tissue in the rumen and reticulum showed similar pattern, which was honeycomb like pattern. This pattern was observed in all portions of the forestomach. Primary wall bordered each cell of the honeycomb. Inside the cells there were some secondary or tertiary wall that connect to the primary ones. The primary wall in the rumen were leaflike shaped with narrow and irregular surface while those of the reticulum were low columnar shaped with convex surface. The honeycomb in the ruminal papillae showed similar pattern from the apical to the basal portion. On the contrary, cone-like primary wall without secondary or tertiary ones dominated the apical portion of the reticulum papillae. The sub epithelial connective tissue consisted of collagen fibers which were arranged and formed a network. The collagen fibers in the rumen were more densely distributed as compared to those of the reticulum. The differences observed may indicate a difference in function between the rumen and the reticulum in the digestive function of the lesser mouse deer.
AH-02 Cutaneous Muscle of Javan Porcupines (Hystrix javanica) Supratikno Supratikno; Halim Bakti Harjo; Danang Dwi Cahyadi; Srihadi Agungpriyono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Cutaneous Muscle of Javan Porcupines (Hystrix  javanica)
AH-25 Sperm Morphology of the Javan Muntjak, Muntiacus muntjak muntjak Sri Wahyuni; Gholib Gholib; Wahono Esthi Prasetyaningtyas; I Ketut Mudite Adnyane; Srihadi Agungpriyono; Hamny Hamny; Muhammad Jalaluddin; Mustafa Sabri; Muslim Akmal; Muhammad Agil; Tuty Laswardi Yusuf
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Sperm Morphology of the Javan Muntjak,  Muntiacus muntjak muntjak
AH-38 Lectin Histochemistry of the Parotid and Mandibular Glands of Barking Deer, Muntiacus muntjak I Ketut Mudite Adnyane; Md Zuki Abu Bakar; Noordin Mohamed Mustapha; Srihadi Agungpriyono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Lectin Histochemistry of the Parotid and Mandibular Glands of  Barking Deer, Muntiacus muntjak
In recent years, there has been an increasing an abuse of slaughtered death chicken for human consumption, so it is important to find a practice ways to identify it. Experiments were conducted to determine whether the quality of meat taken from slaughtered death chicken can be detected through quality attributes of meat (Warner-Bratzler (WB) shear value, CIE L* a* b* color, and histological changes.  Thirty pieces of breast (M. pectoralis) and thigh (M. biceps femoris) meat were obtained from co Razali .; Denny W. Lukman; Srihadi Agungpriyono; Mirnawati Sudarwanto
Forum Pasca Sarjana Vol. 30 No. 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

In recent years, there has been an increasing an abuse of slaughtered death chicken for human consumption, so it is important to find a practice ways to identify it. Experiments were conducted to determine whether the quality of meat taken from slaughtered death chicken can be detected through quality attributes of meat (Warner-Bratzler (WB) shear value, CIE L* a* b* color, and histological changes.  Thirty pieces of breast (M. pectoralis) and thigh (M. biceps femoris) meat were obtained from commercial slaughtering house.  The samples were classified into three groups namely halal slaughtered chicken (AHS), slaughtered death chicken (AMS), and slaughtered stressed chicken (ALS) and all samples processed for microscopic observations, for WB value and color after 1, 5 and 9 h postmortem (PM).  This study showed that percentage of degenerated and necrotic muscle fibres of breast and thigh meat of AMS and ALS were significantly higher (P<0.05) than AHS.  The WB values of all samples were nearly similar (P>0.05).  The L* value of breast and thigh meat of AMS and ALS were lower whereas a* value of breast and thigh meat of AMS were significantly (P<0.05) higher than those of AHS and ALS.  The muscle fiber interstitial space had significant positive correlation with degenerated muscle fiber (P<0.001, r=0.52), necrotic muscle fiber (P<0.001, r=0.57) of breast meat, and also with degenerated muscle fiber (P<0.001, r=0.68) and necrotic muscle fiber (P<0.001, r=0.56) of thigh meat.  The biologic parameters can be used to distinguish between the slaughtered death chicken and halal slaughtered chicken.   Key words: meat quality, muscle fiber, slaughtered death chicken, L* a* b* value
Microscopic Analysis of The Lung in Male Rat (Rattus Norvegicus) Exposed to Cigarettes Smoke Adrien Jems Akiles Unitly; Nastiti Kusumorini; Srihadi Agungpriyono; Aryani Sismin Satyaningtijas; Arief Boediono
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 10, No 2 (2018): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

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Abstract

This research aimed to study on the microscopic changes of lungs in male rat that exposured to cigarette smoke. A factorial CRD with periode of treatment and sample collection was applied in this study. An exposure of cigarette smoke was carried out at 10 cigarettes/rat/day for 2.5 hours in an smoking chamber. Group N is untreated animals. Group 20d is exposed animals with cigarette smoke for 20 days consectitively and released from the cigarette smoke exposure for 20 days. Group 40d is exposed animals for 40 days and released from the cigarette smoke for 40 days. Group 60d is exposed animals for 60 days similar as above. Data collection was carried out twice : after exposure and after redeasing the cigarette smoke exposure. The parameters of observation microscopic analysis of lungs included weight wet of lung and change of lung histopathologic.The results show decreased weight wet of lung, increased number of blackish particulate deposits in the cytoplasm of alveoli at 40d and 60d considered as tar deposit, an increased activity of inflammatory cells of male rats after exposing which were unable to recover after releasing of cigarette smoke exposure.  
Co-Authors A.S. Satyaningtijas Adi Winarto Adrien Jems Akiles Unitly Alastair A Macdonald Amrozi - Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Angelina N. Tethool Anisa Rahma Arief Boediono Aries Boediono Aryani Sismin Sastyaningtijas Asri Pudjirahaju Auzi Asfarian Chairun Nisa Chairun Nisa Chairun Nisa' Chairun Nisa’ Cutnya’ Shaliran Nazlie (Alm) Damiana Rita Ekastuti Danang Dwi Cahyadi Danang Dwi Cahyadi Danang Dwi Cahyadi DENI NOVIANA Denny W. Lukman Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Dondin Sajuthi Dwi Endrawati Dwi Kesuma Sari Dwinna Aliza Erdiansyah Rahmi Erni Sulistiawati Etty Riani Evalina E Fadli A. Gani Gholib Gholib Gunanti . Hafizuddin Hafizuddin Halim Bakti Harjo Hamny Sofyan Helny Rosita Supriadi Helny Rosita Supriadi HERA MAHESHWARI Heru Setijanto Heru Setijanto Huda Salahudin Darusman Huda Salahudin Darusman I Ketut Mudite Adnyana ICHSAN EFFENDIE Idawati Nasution Idawati Nasution Iman Supriatna Ita Djuwita Ita Djuwita Junzo Yamada Ketut Adnyane Mudite Kusdiantoro Mohamad Lidya Elizabeth M. Manik M Agus Setiadi M. Haviz M. Zairin Junior Masaji Washio Md Zuki Abu Bakar Mirnawati Sudarwanto Mokhamad Fahrudin Mokhamad Fakhrul Ulum MOZES R. TOELIHERE MOZES TOELIHERE MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Risman Wahid Mulyadi Adam MULYOTO PANGESTU Muslim Akmal Nabuo Kitamura Najamudin - Nastiti Kusumorini Nastiti Kusumorini Nazlie (Alm), Cutnya’ Shaliran Ni Wayan Kurniani Karja Noordin Mohamed Mustapha Nurhidayat Odilia Rovara R. Iis Arifiantini Razali . RIDWAN AFFANDI Riki Siswandi Ronny Rachman Noor Rusdin - Savitri Novelina Sri Wahyuni Sri Wahyuni Sriyanto - Supratikno Supratikno . Supratikno Supratikno Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Tongku Nizwan Siregar TUTI L YUSUF Tutik Wresdiyati - Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ursula Paulawati Maker Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahyuni, Sri Wartika Rosa Farida Wartika Rosa Farida Yoshio Yamamoto Yuliani Suparmin