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All Journal International Journal of Evaluation and Research in Education (IJERE) Reaktor Abdimas Jurnal Inovasi Pendidikan Kimia Jurnal Bahan Alam Terbarukan Jurnal Penelitian Pendidikan Lembaran Ilmu Kependidikan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Sainteknol : Jurnal Sains dan Teknologi Bulletin of Chemical Reaction Engineering & Catalysis Jurnal Kimia Riset J-PEK (JURNAL PEMBELAJARAN KIMIA) Chemistry in Education Indonesian Journal of Chemical Science Indonesian Journal of Physics and Nuclear Applications Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Innovative Science Education International Journal of Active Learning Jurnal Biomedika Media Farmasi Indonesia Prosiding Seminar Nasional Pascasarjana Proceeding of International Conference on Science, Education, and Technology Bulletin of Chemical Reaction Engineering & Catalysis
Nanik Wijayati
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Biosaintifika: Journal of Biology

 1 
Transformasi ?-Pinena dengan Bakteri Pseudomonas aeruginosa ATCC 25923 Wijayati, Nanik; Astutiningsih, Christina; Mulyati, Suci
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2931

Abstract

Indonesia adalah Negara utama yang memproduksi minyak atsiri di dunia. Minyak terpentin adalah minyak atsiri yang dihasilkan dari destilasi getah pinus Pinus merkusi J ungh. Et. De. Vr. Tujuan penelitian ini adalah untuk meningkatkan nilai minyak terpentin dengan mengubah kandungan utamanya, ?-pinena menjadi senyawa baru menggunakan P. Aeruginosa dalam metode mikrobiologi. Minyak terpentin diambil dari Perhutani Laboratorium Jawa Tengah, dibuat dengan seri konsentrasi 0,5%, 1%, 2%, dan 4%. Minyak terpentin diinokulasi dalam suspensi P. areuginosa selama 48 jam pada suhu kamar (25-28oC). Hasilnya diekstraksi menggunakan dietil eter. Filtrat Terpentin dianalisis menggunakan GCdan IR. Hasil analisis GC menunjukkan puncak baru di konsentrasi 0,5%, 1%, dan 2%, tetapi dalam konsentrasi 4% tidak menunjukkan puncak baru. Hasil IR menunjukkan hidroksil (OH-) dan C-O alkohol. Berdasarkan penelitian ini, dapat disimpulkan bahwa minyak terpentin dapat ditransformasi untuk menjadi senyawa yang mengandung gugus-OH melalui metode mikrobiologi dengan menggunakan bakteri P. aeruginosa.Indonesia is the main producer of essential oil in the world. Turpentine oil is an essential oil which is obtained from pine resin distillation of Pinus merkusi Jungh. et. De.Vr. The aim of this experiment was to increase the value of turpentine oil by changing its main content, i.e. ?-pinene, into a new compound using P. aeruginosa in microbiological method. Turpentine oil was collected from Perhutani Central Java Laboratory, and was made into 0.5%; 1%; 2%; and 4% concentrations and it was inoculated in P. areuginosa suspension for 48 hours in room temperature (25C-280C). The result was extracted using diethylether. The filtrate of turpentine was analyzed using GC and IR. The GC analysis result showed a new peak in 0.5%; 1%; and 2% concentrations, but in the 4% concentration didnt show a new peak. The IR result showed alcohol with hydroxyl (-OH) and CO groups. This experiment concluded that turpentine oil may be transformed using P. aeruginosa in a microbiological method to become a substance containing OH group.
Transformasi α-Pinena dengan Bakteri Pseudomonas aeruginosa ATCC 25923 Nanik Wijayati; Christina Astutiningsih; Suci Mulyati
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2931

Abstract

Indonesia adalah Negara utama yang memproduksi minyak atsiri di dunia. Minyak terpentin adalah minyak atsiri yang dihasilkan dari destilasi getah pinus Pinus merkusi J ungh. Et. De. Vr. Tujuan penelitian ini adalah untuk meningkatkan nilai minyak terpentin dengan mengubah kandungan utamanya, α-pinena menjadi senyawa baru menggunakan P. Aeruginosa dalam metode mikrobiologi. Minyak terpentin diambil dari Perhutani Laboratorium Jawa Tengah, dibuat dengan seri konsentrasi 0,5%, 1%, 2%, dan 4%. Minyak terpentin diinokulasi dalam suspensi P. areuginosa selama 48 jam pada suhu kamar (25-28oC). Hasilnya diekstraksi menggunakan dietil eter. Filtrat Terpentin dianalisis menggunakan GCdan IR. Hasil analisis GC menunjukkan puncak baru di konsentrasi 0,5%, 1%, dan 2%, tetapi dalam konsentrasi 4% tidak menunjukkan puncak baru. Hasil IR menunjukkan hidroksil (OH-) dan C-O alkohol. Berdasarkan penelitian ini, dapat disimpulkan bahwa minyak terpentin dapat ditransformasi untuk menjadi senyawa yang mengandung gugus-OH melalui metode mikrobiologi dengan menggunakan bakteri P. aeruginosa. Indonesia is the main producer of essential oil in the world. Turpentine oil is an essential oil which is obtained from pine resin distillation of Pinus merkusi Jungh. et. De.Vr. The aim of this experiment was to increase the value of turpentine oil by changing its main content, i.e. α-pinene, into a new compound using P. aeruginosa in microbiological method. Turpentine oil was collected from Perhutani Central Java Laboratory, and was made into 0.5%; 1%; 2%; and 4% concentrations and it was inoculated in P. areuginosa suspension for 48 hours in room temperature (25°C-280C). The result was extracted using diethylether. The filtrate of turpentine was analyzed using GC and IR. The GC analysis result showed a new peak in 0.5%; 1%; and 2% concentrations, but in the 4% concentration didn’t show a new peak. The IR result showed alcohol with hydroxyl (-OH) and –C–O groups. This experiment concluded that turpentine oil may be transformed using P. aeruginosa in a microbiological method to become a substance containing –OH group.
Co-Authors - Jumaeri -, Solikhah A.A. Ketut Agung Cahyawan W Afifaturrochmah, Lutfi Agnes Juniarti Chastelyna, Agnes Juniarti Ali Djamhuri Amalina, Nur Dina Anggriani, Frida Anggy Rinela Sulistya Rini, Anggy Rinela Sulistya Anita Harum Sari Antonius Tri Widodo Arief Rohman Arina Marissa, Arina Arofah, Barorotul Ulfah Asbanu, Yoseanno Widi Anugrah Carnawi, Carnawi Christina Astutiningsih Christina Astutiningsih Didik Prasetyoko Drastisianti, Apriliana Dyah Rini Indriyanti Edy Cahyono Eko Budi Susatyo Ella Kusumastuti, Ella Ellianawati, Ellianawati Endang Susilaningsih Enny Ratnaningsih Enny Ratnaningsih Erika Wijayanti Ersanghono K. Ersanghono K., Ersanghono Ersanghono Kusuma Ersanghono Kusumo, Ersanghono F Widhi Mahatmanti Fanny Firman Syah, Fanny Firman Fathur Rokhman Fera Gustri Ariani, Fera Gustri Firstyarikha Habibah, Firstyarikha G, Agnesya Putri Harjito - Harjito Harjito Harjono Harjono Harno Dwi Pranowo Harno Dwi Pranowo Hertiana, Dita Setya Hertiana, Dita Setya Husna Amalana, Husna Hutasoit, Parpulungan Hutasoit, Parpulungan Ika Kusumawati Jumina Jumina Jumina Jumina Kasmadi Imam Supardi Kasmui -, Kasmui Kharomah, Kharomah Kristianingsih, Desiagi Dwi Kurniasari, Laviona Tiara Kurniawati, Erlinda Eka Kusoro Siadi Kusoro Siadi Laily Isni Farikhati Langgeng Setyo Wibowo Lestari, Etika Ayu Lestari, Nanda Ayu Lina Herlina Lina Herlina Lisa Amalia Suryana M. Agus Prayitno, M. Agus M. Aryono Adhi Machmudah - Margareta Dian Purnamasari, Margareta Dian Marsudi Marsudi Mar’attus Solihah Masturi Masturi Melykhatun, Rizka Ayu Mohammad Agus Prayitno Mohammad Agus Prayitno Mohammad Agus Prayitno Muhammad Afif Muharom, Sidik Muharom, Sidik Murbangun Nuswowati Nasution, Annio Indah Lestari Nico Aditya Wijaya Nindya Ayu Lestari, Nindya Ayu Ningrum, Puji Nugroho, Imam Husnan Nuni Widiarti Nur Hidayah Nur Kusuma Dewi Nurul Arifin Oktaviana, Sella Pangestika, Ria Rahayu Pratiwi, Khana Fitri Pratono, Ardiyana Prayitno, Mohammad Agus Purwodiningsih, Ristia Putra, Rizki Suhendar Putri, Syarafina Dirinda Rachmadiyono, Dwi Rahayu, Endah Fitriani Ratna Kumala Dewi Resti, Ade Ria Rahayu Pangestika, Ria Rahayu Ria Wulansarie, Ria Riva Ismawati, Riva Riva lsmawati, Riva Robi’ah Adawiyah, Robi’ah rohayati rohayati Rohmawati, Baiti Romawati, Carolina Romawati, Carolina Rosiyanah, Siti Ruliyanti, Tria S Mursiti Samuel Budi Wardana Kusuma, Samuel Budi Wardana Samuel Budi Wardhana Samuel Budi Wardhana Kusuma Saptorini - Saptorini Saptorini, Saptorini Septiyaningsih, Tri Shakin Ervita Oktaviyani Siti Anisa Rohmah Siti Handayani Siti Sundari Miswadi Solikhah Solikhah Sri Haryani Sri Haryani Sri Ngabekti Sri Supanti Sri Susilogati Sri Susilogati Sumarti SRI WARDANI Suci Mulyati Suci Mulyati Sudarmin Sudarmin Sudarmin Sudarmin Supartono - Suprapto Suprapto Suprapto, Suprapto Susan Primadevi Susanto, Aris Tri Susanto, Aris Tri Sutikno Madnasri Sya'idah, Fatin Atikah Nata Titik Kushandayani Tri Handayani Triawan, Singgih Ade Triawan, Singgih Ade Triyono Triyono Triyono Triyono Tunggul Pawenang, Eram Ulya, Isnaeni Nurul Veronita, Fanna Veronita, Fanna W.D.P Rengga Wahyuningtyas, Wulan Watoni, Edi Satria Wawan Wawan Widarti, Nuni Widya Hary Cahyati Wijianto, Wijianto Woro Sumarni Yayuk Tutiek Supriyanti Yeni Anita Sari yoga agung prabowo Yosepphiene Debbie Damayani Yuliyati, Tri Budi Yunita, Wulansari Zaenuri Zaenuri