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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Jurnal Silvikultur Tropika (JST) Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Bioplante Bumi Lestari Agrikultura Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Keteknikan Pertanian Jurnal Penelitian Hutan Tanaman Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Hexagro Jurnal Bioindustri (Journal of Bioindustry) Jurnal Penelitian Tanaman Industri (Littri) Jurnal Tanah dan Iklim Jurnal Risalah Kebijakan Pertanian dan Lingkungan Proceedings Series on Physical & Formal Sciences

Suryo Wiyono

Department Of Plant Protection, IPB University

Author-ID : 419707

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Social Sciences Veterinary

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Aktivitas Kitinase dan Peroksidase dari Ekstrak Kasar Protein Asal Kalus dan Berbagai Jaringan Tanaman Trichosanthes cucumerina var. anguina Dewi Sukma; Roedhy Poerwanto; , Sudarsono; Nurul Khumaida; I Made Artika; Suryo Wiyono
Indonesian Journal of Agronomy Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Chitinase and peroxydase are important bioactive proteins or are specific enzymes that are related to plant resistance to pathogens. The aims of the research were to analyze the chitinase and peroxidase activities of crude protein extract from calli, stem, leaves and roots of T. cucumerinavar. anguina. In the first experiment, chitinase and peroxydase activities were analyzed from in vitro calli, leaves and roots obtained from 2-month-old of field grown plants. The media for calli induction were Murashige and Skoog medium with addition of 1 µM NAA + 1 µM BA, 2 µM NAA + 2 µM BA, 3 µM NAA + 3 µM BA, or 4 µM NAA + 4 µM BA. In the second experiment, the chitinase and peroxydase activities from crude protein extract of roots, stems and leaves were analyzed. The extracts were from 3-week-old seedling (less than a month), 1-month and 2-month-old plants. The first and the second experiment results showed that crude protein extracts of plant roots from the field grown plants had the highest chitinase and peroxidase activities. Stem of field grown plants had the similar level of chitinase activities with the plant roots. Chitinase activities of in vitro calli were not significantly different from those of plant roots so that it could be used as an alternative for plant roots in studying chitinase from T. cucumerinavar. anguina. Chitinase activities in crude protein extracts of roots appeared constant whereas peroxidase tend to increase with plant age. Keywords: calli, enzyme activities, leaves, roots, stem

Pemanfaatan Khamir Antagonis untuk Memperpanjang Umur Simpan dan Mengendalikan Penyakit Antraknosa Buah Pepaya Mutiara Dwi Lestari; Ketty Suketi; Winarso Drajad Widodo; Suryo Wiyono
Indonesian Journal of Agronomy Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Papaya is one of the climacteric fruits that has a short shelf life and potential for postharvest anthracnose disease disorders caused by the pathogen Colletotrichum gloeosporioides. The purpose of this study was to investigate the effectiveness of several antagonistic yeast species to extend shelf life and controlling anthracnose disease in postharvest of Callina papaya. This research was conducted at the Papaya field in Kanaga Village, Lebak Regency, Banten from November 2018 to July 2019, and Postharvest Laboratory AGH, IPB University from March to July 2019. The experiment was arranged in a randomized complete block design with the treatment of five types of yeasts, i.e., Cryptococcus albidus Yp, Aureobasidium pullulans Dmg 30 DEP, Rhodotorula minuta Dmg 16 BEP, Candida tropicalis Lm 13 BE, and Pseudozyma hubeiensis Dmg 18 BEP, a fungicide treatment with active ingredient azoxystrobin as a standard, and without treatment as a control. The results showed that the use of Candida tropicalis, Aureobasidium pullulans, and Cryptococcus albidus extended the shelf life of Callina papaya fruit to 12.6, 12.4, dan 12.2 days, respectively, longer than the control that was only 7 days, and effectively prevented anthracnose disease with suppression rates of 58.33%, 54.17%, and 50.00%, respectively, during storage. The use of yeast antagonists did not affect the physical and chemical quality of Callina papaya. Keywords: climacteric fruit, Colletotrichum gloeosporioides, postharvest

Penambahan Berbagai Jenis Pupuk Organik dan Pupuk Hayati terhadap Produktivitas dan Mutu Benih Kedelai (Glycine max.L.) . Rifka; Memen Surahman; Suryo Wiyono
Buletin Agrohorti Vol. 7 No. 3 (2019): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Penelitian ini bertujuan mendapatkan jenis pupuk organik untuk meningkatkan produktivitas dan mutubenih tanaman kedelai (Glycine max L.). Penelitian dilaksanakan di Kebun Percobaan Leuwikopo, InstitutPertanian Bogor, Dramaga, Bogor pada bulan Desember 2017-April 2018. Jenis tanah Kebun PercobaanLeuwikopo yaitu latosol. Rancangan perlakuan yang digunakan adalah faktorial satu faktor dalam rancanganlingkungan kelompok lengkap teracak dengan empat ulangan. Faktor yang diamati adalah jenis pupuk yangterdiri atas enam taraf yaitu perlakuan P0 (tanpa pupuk anorganik dan pupuk organik), P1 (Pupukanorganik), P2 (Abu sekam bakar), P3 (Bio organik), P4 (Pupuk organik cair) dan P5 (Plant growthPromoting Rhizobacteria (PGPR)). Pengujian mutu benih hasil produksi dilaksanakan di LaboratoriumPengujian dan Penyimpanan Benih pada bulan April 2018. Pengujian tersebut terdiri atas pengujian dayaberkecambah, indeks vigor benih, potensi tumbuh maksimum, berat kering kecambah normal dan bobot 100butir benih. Hasil penelitian menunjukan bahwa perlakuan pupuk organik tidak berpengaruh terhadapkomponen pertumbuhan vegetatif seperti tinggi tanaman, jumlah cabang per tanaman, dan jumlah daunterkecuali daya tumbuh dan jumlah bintil akar. Perlakuan pupuk memberikan pengaruh nyata terhadapproduksi kedelai dan bobot 100 butir benih tetapi tidak berpengaruh nyata terhadap jumlah polong pertanaman, jumlah polong isi, bobot polong isi, bobot biji tanpa polong, jumlah butir per tanaman, jumlahcabang produktif, dan bobot akar. Perlakuan pupuk tidak memberikan pengaruh nyata pada komponenpengujian mutu benih yakni variabel daya berkecambah, indeks vigor, potensi tumbuh maksimum dan bobotkering kecambah normal. Penambahan pupuk organik yang menghasilkan produksi terbaik yaitu perlakuanPGPR dengan dosis 10 g kg-1 benih. Semua perlakuan pupuk organik tidak berpengaruh terhadap mutufisiologis benih.

Formulasi Tepung Biofungisida Berbahan Aktif Ganda Pseudomonas Fluorescens PG 01 dan Bacillus Polymixa BG 25 . Widodo; Suryo Wiyono
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 3 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (761.256 KB)

The objective of this study is to determine effective carrier materials and additives which is able to keep the bioperformance, including antibiosis activity to Phytophthora capsici and Colletotrichum acutatum and plant growth promoting effect, of two antagonistic bacteria Psedomonas fluorescens PG 01 and Bacillus polymixa BG 25 in biofungicide powder formulations. Talc and bentonite formulations were effective after 3 months of storage, while tapioca were only effective to B. polymixa up to 3 months of storage. Additive materials that can enhance the antibiosis activity of the bacteria, keeping up the growth and no toxicity effect to chili seedlings were crab shell powder 0.25% and MnSO4 1 to 2%. After eight months storage with 20% moisture content, the bacteria population survived in powder formulation developed in this study was still suitable for seed treatment and/or after transplanting through soil drenching with water. In this period of storage, population of the two bacteria was 106 cfu/g formulation.

Keanekaragaman Mikrob Fungsional Rizosfer Nanas dengan Berbagai Tingkat Produktivitas Aditya Dyah Utami; Suryo Wiyono; Rahayu Widyastuti; Priyo Cahyono
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 4 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.4.584

Functional microbes of rhizosphere play important roles in nutrient transformation and controlling disease as well as in supporting plant growth and development. However, there is no study on the role of functional microbes on pineapple productivity. The purpose of this study was to investigate the abundance and diversity of soil functional microbes at different growth phases at two levels of productivity and their correlations to disease incidence. The research process included sampling of pineapple rhizospheric soil from vegetative and generative phases pineapples at low and high plant productivity sites, observations of disease incidence, and isolations of functional microbes. Functional groups of bacteria were Azotobacter, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, antibiotics-producing bacteria, IAA-producing bacteria, and chitinolytic bacteria. The soil sampling method was simple randomized sampling at 6 locations with an area of each location ± 5 ha with a depth of 20 cm. Rhizosphere were taken in plants grown in high productivity area (>60tons/ha) and low productivity area (<60 tons/ha) in vegetative and generative phases. The results showed that potassium-solubilizing bacteria, chitinolytic bacteria, and IAA-producing bacteria were more abundant during the generative phase compared to those during vegetative phase. While Azotobacter, phosphate-solubilizing bacteria, and antibiotic-producing bacteria were more predominant during vegetative phase at various crop productivy. Total density of microbes was higher in soil with high crop productivity than that in soil with low crop productivity. The abundance of chitinolytic bacteria and IAA-producing bacteria had negative correlation with disease caused by Erwinia chrysanthemi and Phytophthora cinnamomi. Keywords: chitinolytic bacteria, growth phase, IAA, pineapple disease

Dampak Penggunaan Fungisida Sintetik pada Kelimpahan Cendawan Endofit Tanaman Padi Rika Estria Gurusinga; Lilik Retnowati; Suryo Wiyono; Efi Toding Tondok
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.432

Application of fungicides to control rice diseases potentially have adverse effect on non target organisms including endophytic fungi. Unfortunately, information of fungicide effect on endophytic fungi on rice is still limited. The purpose of this research was to study the diversity and abundance of endophytic fungi on rice plant because of application of synthetic fungicides. There were two treatments in this study: non systemic fungicide (mankozeb) and systemic fungicide (difenoconazole) which were compared to control (without fungicide). Isolation of endophytic fungi and application of fungicides were conducted every week started at aged of 3 to 10 weeks after planting. Variables observed were total segment of rice colonized by endophytic fungi, colonization frequency of endophytic fungi, continued by identification and analysis of the diversity of endophytic fungi. This study showed that there was an increase in colonization of endophytic fungi with an increase of plant ages. The diversity of endophytic fungi was higher in stems than in leaves with the value of H' is 0.00-1.35 and 0.00-0.98 respectively. The most common types of endophytic fungi appear in each isolation of rice segments were Acremonium, Curvularia, Fusarium, Helminthosporium, Nigrospora, Penicillium, and Mucor. Both fungicides do not significantly affect the percentage of colonization and diversity of endophytic fungi. At the beginning of planting (4-6 MST) there was a decrease in the percentage of endophytic fungal colonization in the stem. From the percentage of colonization of each genus of fungi, Nigrospora and Fusarium decreased significantly with difenoconazole on the stem during the vegetative phase. Keywords: colonization, community, difenoconazole, diversity, mancozeb

Kajian Penerapan Teknologi Pengendalian Penyakit pada Pembibitan Sengon di Bogor Arif Ravi Wibowo; Suryo Wiyono; Anna Fariyanti
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.269

Sengon (Falcataria moluccana) cultivations still have to face threats from plant diseases. Diseases can be found in nurseries and plantation. Biological agents can be used to control various types of plant diseases and have various advantages. The use of biological agents in forestry plantations must consider the aspects of effectiveness and efficiency. This study aims to examine the effect of various treatment of control technology packages on the incidence of major diseases, and to determine the financial feasibility of various disease control technology packages for sengon in nurseries. The research was conducted through interviews to explore cultivation techniques and production costs. In addition, experiments were carried out on the application of various disease control strategies using commercial formulations of biological agents. The commercial formulations of biological agents used were Rhizomax, Primagrain, Trichowish, Kayabio, Bio-Hara Plus, and fungicide Dithane M-45 80 WP. There were two treatment packages of new technology that have an effect on increasing the growth of sengon at the beginning of growth stage, namely P1 (BioHara Plus and KayaBio) and P2 (Rhizomax and Primagrain) treatments. All treatments of the new technology package had no significant effect on increasing the survival rate and suppressing the incidence and severity of disease in sengon seedlings. All new technology package treatments can generate profits even though the value is smaller when compared to existing technology treatments. All the new technology package treatments have an R/C ratio of more than 1 so that they are feasible to be applied by farmers. Keywords: biological control, feasibility, plant diseases, nurseries, Falcataria moluccana

Penyebab Penyakit Layu pada Tanaman Semangka di Karawang, Jawa Barat Kartini Budiastuti; Efi Toding Tondok; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 8 No 4 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Wilt disease of watermelon is an important disease in Indonesia, but determination of the causal agent has not been reported yet. Postulat Koch based approach was conducted to determine the pathogen causing wilt of watermelon from Karawang, West Java. Fungi associated with wilt symptoms were isolated from stem and root, then the fungal colonies were identified based on their morphological characters. Pathogenicity tests were performed using soil media and wet paper. Host range study involved other Cucurbitaceae plants, i.e. cucumber, melon and paria. Three species of Fusarium were successfully isolated from plants showing wilt symptom, i.e. F. oxysporum, F. solani, and F. semitectum. The fungus F. oxysporum consistently caused the most severe wilt symptom on watermelon, but developed no symptom on other tested cucurbits. The two fungi, F. solani and F. semitectum, caused necrotic on roots and stem of watermelon followed by wilting of the plant. The symptom was different than those of the initial symptoms from the field. Therefore it concluded that wilt symptom of watermelon is caused by Fusarium oxysporum f.sp. niveum. Key words: Cucurbitaceae, Fusarium oxysporum f.sp. niveum, species identification, wilt

Penyakit Keriting Daun Pepaya yang Disebabkan oleh Cladosporium cladosporioides Widodo .; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Leaf curling and chlorotic spot on young leaves of papaya type California was observed in Bogor, Kebumen, and Bekasi. Conidium of Cladosporium cladosporioides was found associated with diseased tissue based on microscopic examination. To our knowledge, this is the first report of C. cladosporioides causing papaya leaf curling in Indonesia. Key words: chlorotic, leaf curl disease, papaya disease, scab

Laporan Pertama tentang Penyakit Busuk Arang pada Kacang Tanah Suryo Wiyono; Widodo .
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Peanut charcoal rot disease was first observed in Bogor,West Java in June 2010, and Serang, Banten in January 2012. Infected plants in the field showed root and stem rot, discoloration of stems and/or branches. Severe infection may cause wilting of foliage then plant dried out. Based on microscopic observation Macrophomina phaseolina was constantly associated with diseased plants from those two areas. Hot temperature and low rainfall might favour disease development. This is the first report of charcoal rot disease of peanut in West Java and Banten. Key words: charcoal rot, Macrophomina phaseolina, peanut

Co-Authors . Giyanto . Rifka . Widodo . Widodo . Widodo Abdjad Asih Nawangsih ABDJAD ASIH NAWANGSIH Abdul Munif Agus Purwito Ahmad Fauzi Ridwan Ahmad Sutopo ALI NURMANSYAH Ali Wafa Aloysius Rusae Anas Dinurrohman Susila Andika Septiana Suryaningsih Andika Septiana Suryaningsih Andriani, Desta Anna Fariyanti Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R ARI SUSILOWATI Arif Ravi Wibowo Aris Tri Wahyudi Astika Widhi Pratiwi Atit Kanti Atit Kanti Awang Maharijaya Baskoro Sugeng Wibowo Bonny Poernomo Wahyu DADANG DADANG Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewi Sukma DWI ANDREAS SANTOSA Dyah Hariyanti Purnomo Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Elis Nina Herliyana Eryna Elfasari Rangkuti Evan P. Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Fany Juliarti Panjaitan Febbiyanti, Tri Rapani Firman Hidayat Fitri Kemala Sandra Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Heny Setiyowati Heri Harti Heri Harti Hermanu Triwidodo I MADE ARTIKA I Made Sudiana I Made Sudiana I Nyoman Sumerta Ida Parida Idris Idris IKA DAMAYANTI Iskandar Z. Siregar Islah Hayati Istiaji, Bonjok Ita Aprilia Ivone Oley Sumarauw Juang Gema Kartika Kartini Budiastuti Ketty Suketi Ketty Suketi Khoirotul Afifah Kikin H Mutaqin Kristianto Nugroho Kristianto Nugroho Kudang Boro Seminar Kusuma Darma Kusuma Darma Lilik Retnowati Lina Herlina Lina Herlina Listihani, Listihani Masrukhin Masrukhin Mei Lita Fitriani Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Surahman Mutiara Dwi Lestari Napiudin Nazly Aswani Neo Endra Lelana nFN Khaerati Nika Ropiatningsuari Nur Alfi Saryanah Nurul Khumaida Phor Bho Ayuwati Priyo Cahyono Puji Lestari RAHAYU WIDYASTUTI Reflinur Reflinur Reflinur Reflinur Remaja Sitepu Rerenstradika T. Terryana Riana Jumawati Rika Estria Gurusinga Rizki Abi Amrullah ROEDHY POERWANTO Roza Yunita Rustam, Rustam Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Silviana Arsyad Sitti Eha Faihah Sitti Fadhilah Slamet Susanto Sobir Sobir Sobir Sobir Sri Hartati Sri Hartati Sri Hartati SRI HENDRASTUTI HIDAYAT stephanie stephanie Sudarsono SUDIRMAN YAHYA Suryahadi Suryahadi SUSILOWATI1 SUSILOWATI1 Suwarno . Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Thamrin Khamidi Titiek Siti Yuliani Toga Pangihotan Napitupulu Tondok, Efi Toding TRI ASMIRA DAMAYANTI Tri Rapani Febbiyanti, Tri Rapani Ummu S. Rustiani Ummu Salamah Rustiani Utami, Aditya Dyah UTUT WIDYASTUTI Vinsen Willi Wardhana Wartono Wartono Wawan Setiawan Wawan Setiawan Weni Wilia Widodo Widodo . Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widya Sari Winarso D. Widodo Winda Ika Susanti Yayu Siti Nurhasanah Yuli Fitriati Yuliawati YULIN LESTARI Yundari, Yundari Yuyun Andriyani

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