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Journal : Jurnal Medika Veterinaria

1. Immunohistochemical Study of the Immunoreactive Follicle Stimulating Hormone (ir-FSH) Cells Distribution in Pituitary Gland of Rat(Rattus norvegicus) Patrick Flaggellata; Sri Wahyuni; Muhammad Jalaluddin; Hamny Hamny; Gholib Gholib; T. Armansyah, TR; Muslim Akmal
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4092

Abstract

The objective of this study was to identified the development of the immunoreactive follicle stimulating hormone (ir-FSH) cells in pituitary glands of rats (Rattus norvegicus)in different age using immunohistochemical (IHC) methods. The pituitary organ that used in this study was collected from eight white female rats aged 2, 4, 6, and 12 months. Pituitary glands were processed histologically and stained with IHC methods to detect the ir-FSH cells. The result showed that the ir-FSH cells were observed in pars distalis (PD), pars tuberalis (PT), and pars intermedia (PI) of adenohypophysis with different number of cells. Furthermore, ir-FSH cells were found abundantly (+++) in the PT and a few (+) in PD of rats aged two months. In rats aged 4 months those cells were distributed in slight number (+) in PT and abundant (+++) in PD. In addition, the cells were also found in a great quantities in rats whose ages were 6 months, (+++) in both PT and PD, but these cells decreased (++) in rats aged 12 months in PD and absent (-) in PT. In conclusion, the devalopment and distribution of ir-FSH cells were found in pars tuberalis and pars distalis adenohypophysis in the pituitary glands of rats aged 2 up to 12 months with different number and distribution patterns.
3. An Immunohistochemical Study of Alpha Estrogen Receptor (ERα) Development in Ovary and Uterus of Rat (Rattus norvegicus) Sri Wahyuni; Chintya Desfariza; Hamny Hamny; Muslim Akmal; Gholib Gholib; T. Armansyah
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4100

Abstract

This research aimed to observe and identify the development of estrogen receptor alpha (ERα) in the ovary and uterus of rats (Rattus norvegicus) at various age levels using immunohistochemistry (IHC). The ovary and uterus were collected from eight female rats aged 2, 4, 6, and 12 months and processed histologically and stained with IHC staining. This research showed, the intensity of ERα expression was found in high abundance (+++) in stroma and the corpus luteum and was weakly found (+) in the theca cells, blood vessels, oocytes and germinal epithelium of rats aged 2 months. Additionally, ERα expression in rats aged 4 months had similarities with rats aged 2 months, but the intensity of ERα had increased (++) in ovarian theca cells, blood vessels, and oocytes. In ovaries of rats aged 6 and 12 months, ERα expression had decreased (++) in the stroma and the corpus luteum and had a low expression (+) in the blood vessel and oocytes. The uterus of rats aged 2 months had low expression of ERα (+) in the stroma and luminal epithelium, moderate (++) in the myometrium, but was not found in the uterine glands. Furthermore, the expression in the uterus of rats at the age of 4 months had increased in the stroma and luminal epithelium (++) and uterine glands (+).  At the age of 6 months, it found excessively (+++) in the stroma, myometrium, and luminal epithelium, moderate expression (++) in the uterine glands.  The expression of ERα in uterus aged 12 months was almost equal to the uterus of rats aged 6 months, but the expression in the myometrium and epithelial luminal had decreased (++). It can be concluded that the development and distribution pattern of ERα in the ovaries and uterus of rats are different at each age levels, it may be related to the growth and development of reproductive organs and also closely related to the estrus cycles of rats.
The Effect of Jaloh (Salix tetrasperma Roxb) Extract on Fat level and Water Content in muscle of Broiler Chicken which Heat Stres Condition Yana Zein; Sugito Sugito; Amiruddin Amiruddin; Roslizawaty Roslizawaty; Gholib Gholib; M. Isa
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.18971

Abstract

Exposure to heat conditions is one of the causes of heat stress for broilers. This research was conducted to determine the effect of administering jaloh extract (Salix tetrasperma Roxb) in drinking water to broiler chickens with heat stress condition on fat levels and muscle water content (intramuscular). This research was conducted using a completely randomized design with a sample of 15 broiler chickens strain MB 90 aged 21 days which placed in individual cages and a temperature of 34 ± 1ºC was set for  4 hours/day for 7 consecutive days. The treatment groups were as follows: P0 as control group without jaloh extract; P1 was given jaloh extract at a dose of 50 mg/l; P2 was given jaloh extract at a dose of 100 mg/l in drinking water. Each treatment group consisted of 5 replications. The data were analyzed using ANOVA test, and then continued with the Duncan test. The results showed that jaloh extract at a dose of 100 mg/l in drinking water could increase the fat content of meat up to 16.63% and could reduce the water content to 44.52%. It could be concluded that the administration of jaloh extract at a dose of 100 mg/l in drinking water could increase fat levels and reduce intramuscular muscle water content in broiler chickens with heat stress condition
3. The Effect of Audio Recording for Playback Experiment to the Alteration of Orangutan’s Territorial Behavior Rahmat Nazif; Gholib Gholib; Erdiansyah Rahmi; Arman Sayuti; Triva Murtina Lubis; Ummu Balqis
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4253

Abstract

The aim of this study was to find an alternative method to prevent the conflict between the orangutan and human with the utilization of audio recording as biofence. The location of this study was in Leuser National Park on Bukit Lawang, Langkat, North Sumatra using 16 orangutans as the subject of this study. The method used in this study was a simulation and playback experiment which were using long call, spontaneous call, predator voice, and siren sound in the audio recording of non-alpha orangutans. The respond was observed using binocular and recorded using camera recorder. The data obtained were analyzed using Krustal Wallist test. The result showed that 87% of orangutan showed an interest in the attractor, on the other hand, 13% showed disinterest to the attractor. The facial expression shown by orangutan was fear, submission, aggression, and worry. The statistical analyzing result showed that long call and the spontaneous call was very significant (P0.01), while the predator and siren voice were insignificant (P0.05). The result can be concluded that long call and spontaneous calls showing the effective result to the alteration of territorial behavior on orangutan and have a potential as a biofence that is used as an alternative method to prevent the conflict between orangutan and human.
7. Total Level of Hemoglobin and Hematocrit Value of Broiler Chickens after Given Moringa Leaves Fermented Flour (Moringa oleifera) in Feed M. Hasan; Octa Sicillia Rampai; Arman Sayuti; Razali Daud; Abdul Harris; T. Armansyah TR; Gholib Gholib
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.4299

Abstract

This study aimed to determine the effect of using Moringa leaves fermented flour in feed stock toward the increasing level of hemoglobin and hematocrit value on broiler chickens. This study used a completely randomized design that consists of 4 treatments and 5 replications. The study group was divided into group P0 which was given with 100% of commercial feed, group P1 was given 95% of commercial feed and Moringa leaves fermented flour 5%, group P2 was given 92.5% of commercial feed and Moringa leaves fermented flour 7.5%, and group P3 was given 90% of commercial feed and Moringa leaves fermented flour 10%. Fermentation using EM4 (Effective Microorganism-4) were able to decrease the crude fiber of Moringa leaves flour. The treatment was conducted for 15 days, then hemoglobin levels and hematocrit values were counted. Data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. This research showed that giving Moringa leaves fermented flour for 15 days did not have a significally effect (P0.05). The given Moringa leaves fermented flour in  feed for 15 days with 5%, 7.5% and 10% concentration was not show any increase in the levels of hemoglobin and hematocrit values.
16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract Rizki Aulia; Sugito Sugito; M. Hasan; T. Fadrial Karmil; Gholib Gholib; Rinidar Rinidar
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.4667

Abstract

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella
Co-Authors . Darmawi . Erina . Ismail . Nurliana Abdul Harris Abdullah Hamzah Afifuddin Afifuddin Al Azhar Amiruddin A Amiruddin Amiruddin Andre Pasetha Antje Engelhardt Antje Engelhardt Arman Sayuti Arman Sayuti Azhar A Bambang Purwantara Budianto Panjaitan Budianto Panjaitan Chintya Desfariza Cut Dahlia Iskandar Cut Erika Ramadhana Cut Nila Thasmi Cut Nila Thasmi Dasrul Dasrul Dasrul Dasrul Dian Masyitha Dwinna Aliza DYAH PERWITASARI -FARAJALLAH Eka Meutia Sari Erdiansya Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Fadli A. Gani Fadli A. Gani Farida Athaillah Fashihah Rahmah Noya Farimansysh Fisma Amri H Alief Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hamny Sofyan Hanifa Yusuf HANIFAH YUSUF Hasanuddin Hasanuddin Herrialfian Herrialfian Husnurrizal . Ika Inda Bani Iman Supriatna Joharsyah J Juli Melia Juli Melia Ketut Adnyane Mudite Lilis Suryani M Adam M Hamdan M Hasan M Syafruddin M. Hasan M. Hasan M. Isa Mahdi Abrar Mahdi Abrar Michael Heistermann Muhammad Adlim MUHAMMAD AGIL Muhammad Bahi Muhammad Hambal Muhammad Hambal Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin Mulkan Mulkan Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam Muslim Akmal Muslim Akmal Muslim Akmal Nabilah Putroe Agung Nanda Yulian Syah Nellita Mutia Novika Ayuni Rambe Nuzul Asmilia Octa Sicillia Rampai Okta Hilda Kadar Patrick Flaggellata Putri Handayani Putri Handayani Rahma Melinda Rahmat Nazif Rasmaidar Rasmaidar Razali Daud Razali Daud Rinidar R Rinidar Rinidar Riski Ananda Rizki Aulia Rosa Rika Wahyuni Roslizawaty R Roslizawaty Roslizawaty Rumi Sahara Zamzami Saifan Nur Sema Coumandary Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Sugito Sugito Suriadi S Syafruddin S Syafruddin Syafruddin Syifa Salsabila T. Armansyah T. Armansyah T. Armansyah TR T. Armansyah, TR T. Fadrial Karmil Taufiq P. Nugraha Taufiq Purna Nugraha Teuku Armansyah Teuku Armansyah Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Triva Murtina Lubis Triva Murtina Lubis Tuty Laswardi Yusuf Ummu Balqis Usma Aulia Utari Desya Wahono Esthi Prasetyaningtyas Wahyuni, Sri Yana Zein Yezi Gita Rahayu Zainuddin Z ZK Abdurahman Baizal Zuhrawati Zuhrawati Zulkarnain Zulkarnain