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All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Jurnal Penelitian Tanaman Industri (Littri) Agrokompleks

SEDYO HARTONO

Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281

Author-ID : 3094341

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Preferensi Wereng Batang Cokelat terhadap Varietas Padi dan Ketahanan Varietas Padi terhadap Virus Kerdil Hampa Suprihanto, Suprihanto; Somowiyarjo, Susamto; Hartono, Sedyo; Trisyono, Y. Andi
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Rice ragged stunt virus (RRSV) is transmitted by brown planthopper in the persistent manner. This disease in recent years has become a serious problem in Indonesia and some other countries, such as China, Vietnam, Philippines, and Thailand. Disease control is usually conducted by the vector control using insecticides, so often causes an environmental pollution. An alternative control method is using the environmentally friendly of resistant varieties. The purpose of this study was to determine the level of preference of brown planthopper (BPH) to some varieties and rice germplasms, the resistance of several varieties against rice ragged stunt virus (RRSV) disease and its effectiveness as an inoculum source of virus (RRSV). A total of 15 varieties of rice were used in preference test of BPH and resistance test to RRSV. The test for resistance varieties to RRSV was conducted by transmission of 2nd instar of BPH for 3 days of acquisition feeding period, 10 days incubation period and 24 hours inoculation period with population density of 3 BPH/plant. Disease index was calculated and used to determine the level of plant resistance. Varieties that showed resistant, moderately resistant and susceptible responses were selected and were used as a source of inoculum to be transmitted on to TN1 variety susceptible check variety to know the effectiveness of varieties as source of virus inoculum. The results showed that of the 15 varieties tested, Situ Bagendit, Utri Merah, Mentik Wangi, Mahsuri, and Inpari 1 each was less favored by BPH to settle and to multiply. Mentik Wangi, Tetep, Utri Merah, and Swarnalata each showed resistant response to RRSV. Transmission test to susceptible variety (TN1) showed that the variety of Situ Bagendit, Inpari 13, Mentik Wangi, and Tetep each has a fairly low effectiveness as a source of inoculum as indicated by the lower percentage of infection and disease index on the transmited test plants.

Keragaman Virulensi dan Konstruksi Molekuler Virus Tungro pada Padi dari Daerah Endemis Praptana, R. Heru; Sumardiyono, Y. B.; Hartono, Sedyo; Trisyono, Y. Andi; Widiarta, I Nyoman
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Tungro is an important disease of rice, constraining to the rice production in Indonesia. Tungro is caused by the infection of two different viruses namely tungro bacilliform virus (RTBV) and tungro spherical virus (RTSV). Both viruses are only transmitted by green leafhoppers, especially Nephotettix virescens in a semipersistent manner. The variation of tungro viruses from different areas had been reported, and there is a specific relationship between resistance variety and tungro virus isolate. It is important therefore, to study the virulences and the genetic diversities of tungro viruses derived from the endemic areas in Indonesia. This study was aimed to identify the virulence and the molecular diversity of tungro viruses from endemic areas in Indonesia. Susceptible variety TN1 was used in the study. Surveys and collection of the infected plants and green leafhoppers were conducted in some tungro endemic areas, including: West Java, Central Java, Yogyakarta, Central Sulawesi, West Sulawesi, South Sulawesi, Bali and West Nusa Tenggara. Artificial virus transmission using test tube method was used in the virulence test. Green leafhoppers caught from the field were used as vector transmitters. The virulence of tungro viruses was determined based on diseases indexes (DI). Results showed that the virulence of tungro viruses varied among region in the endemic areas in Indonesia. The Central Java virus isolate was the most virulence; however, not all isolates from endemic areas in the island of Java were more virulent than those from outside of Java. The presence of RTBV and RTSV was detected in the infected TN1 plants. The existences of molecular diversities of tungro viruses from the endemic areas were observed. The relationship between combination of DNA bands of RTBV and RTSV with the virulence in endemic areas outside of Java was more complex than it was in West Java and Central Java. The molecular diversities of tungro viruses were not correlated with the geographic difference of the endemic areas, nor with the virulences.

Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika Andi Khaeruni Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Budi Setiadi Daryono Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika Gusnawaty HS GUSNAWATY HS, GUSNAWATY Hasdiana Hasdiana Helina, Selvi Heri Widarta I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Botek Muhammad Muhsin MUHAMMAD TAUFIK Muhammad Taufik Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nur Isnaini Ulfa Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng Rahma Ayu Priani Resti Fajarfika Retno Mastuti Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siti Anima Hisein Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono

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