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All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Jurnal Penelitian Tanaman Industri (Littri) Agrokompleks
SEDYO HARTONO
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

 1 
DETECTION AND TRANSMISSION OF RICE STUNT VIRUS ON CIHERANG AND SITU BAGENDIT VARIETIES Helina, Selvi; Sulandari, Sri; Hartono, Sedyo; Trisyono, Andi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 2 (2018): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.425 KB) | DOI: 10.23960/j.hptt.218169-176

Abstract

Detection and Transmission of  rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.
DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD Astuti, Suryani Titi; Sulandari, Sri; Hartono, Sedyo; Somowiyarjo, Susamto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12156-62

Abstract

Detection and identification of yellow mosaic stunt disease on Petunia sp. using nested PCR method. Yellow mosaic stuntdisease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. Thedisease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of thedisease may be carried over to imported seeds and necessary to identify as a basic information for developing controlstrategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents wasconducted using electron microscope with quick dipping method and the molecular detection was done using nested PCRwith TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculationshowed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and veinbanding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sizedapproximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis,the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, aplant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the firstreport of ReMV in Indonesia infecting Petunia as ornamental plant.
Recombinant antibody production by cloning of Pepper yellow leaf curl Indonesia virus (PepYLCIV) coat protein gene Yashanti Berlinda Paradisa; Sri Sulandari; Sedyo Hartono; Susamto Somowiyarjo; Mery Windarningsih; Dini Wahyu Kartika Sari; Christina Retna Handayani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.1221-13

Abstract

Pepper yellow leaf curl Indonesia virus (PepYLCIV) is an important pathogen on chili cultivation and is transmitted through the seed. Serological tests are sensitive, accurate, efficient and it has been widely used for the detection of seed-transmitted plant viruses. This study aimed to produce PepYLCIV recombinant protein as a material to produce recombinant antibodies PepYLCIV. DNA was extracted from infected chili leaves collected from Congkrang, Muntilan, Central Java verified using primer PepYLCIV-BamHI and PepYLCIV-EcoRI and produced an amplicon at 840 bp. The amplified fragments were cloned into the pET32a then transformed to Escherichia coli BL21. The percentage of nucleotide sequence identity and sequence of amino acid, PepYLCIV CK-6 isolates had the highest similarity of nucleotide and amino acid sequences to of chili isolates from Bandung. The expressed recombinant protein was obtained with IPTG concentration 0,5 mM and harvested at 6 hours after IPTG induction. SDS PAGE analysis of the recombinant plasmid Begomovirus CK-6 showed that the coat protein size was about 29 kDa. Immunization was carried out on rabbits by injecting 150 µg of recombinant protein 4 times with an interval of 1 week to produce crude antiserum and pure antiserum capable of detecting PepYLCIV in chili and Ageratum conyzoides using I-ELISA and DIBA tests.
Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika Andi Khaeruni Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Budi Setiadi Daryono Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika Gusnawaty HS GUSNAWATY HS, GUSNAWATY Hasdiana Hasdiana Helina, Selvi Heri Widarta I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Botek Muhammad Muhsin MUHAMMAD TAUFIK Muhammad Taufik Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nur Isnaini Ulfa Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng Rahma Ayu Priani Resti Fajarfika Retno Mastuti Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siti Anima Hisein Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono