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Mustofa Mustofa
Departement of Pharmacology, Faculty of Medicine, University Gadjah Mada, Yogyakarta
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Journal : Indonesian Journal of Biotechnology

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An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes ., Alimuddin; Asmara, Widya; Widada, Jaka; ., Mustofa; Nurjasmi, Reni
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words : pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizosphere
Co-Authors Abdul Manaf Ali, Abdul Manaf Ade Setiawan Agus Sugiarto Agussalim Ahmad Hamim Sadewa Ahmad Khoirul Fata Akhwani Alatas, Haidar Alexis Valentin, Alexis Alfi Yasmina Alfiyah, Viny Ali Djamhuri Ali Ghufron Mukti Andayani, Tri M. Andi Hairil Alimuddin Andi Namirah Muh. Syuaib AS, Andi Andrian Saputra Ange Desire Yapi, Ange Desire Arief Budiyanto Astuti, Rachmawati Darma Awal Syahrani Sirajuddin Basri Basri Bawazier, Lucky Aziza Chairil Anwar Danny Syamsu W Danto, Danto Desi Suci Handayani Dessy Susanti, Inggit Dewi Sulistiyarini Dhina Fitriastuti Djaswadi Dasuki Djoko Wahyono Dwi Siswanta Edy Turjono Endah Puji Septisetyani, Endah Puji Endang Darmawan Erna Kristin Eva Rahmawati, Eva Evi Mulyani Faiz, Afiyatul Mahfudloh Faqih, Muhammad Izzatul Fredie Irijanto Gunawan Gunawan Hafizah, Noor Hanifah Yusuf Heriyanto Heriyanto Hidetaka Kawakita Idris, M. Manshur Ika Puspita Sari Indah Purwantini Indwiani Astuti Iqmal Tahir JAKA WIDADA Jastria Pusmarani Jenny Hidayat Jumina Jumina Keisuke Ohto Kennedy, Kennedy Khoirul Fata, Khoirul Kristian Selleng Leo Soemardji Listini Listini, Listini Lorettha Wijaya Mahardika Agus Wijayanti Marhadi Saputro Masrid Pikoli Mintarsih, Betty Mintarsih, Betty Misba Huddin Mohamad, Roni Muhammad Idham Darussalam Mardjan Mulyani Surendra Muthi Ikawati Muvita Rina Wati Nazudin, Nazudin Nita Suleman Novita Carolia Parwanti, Sri Pekerti UA, Pekerti Purwandari, Elce Putra, Eriek Aristya Pradana Putri, Depi Rahmani, Mawardi Rahmani, Mawardi Ratna Asmah Susidarti Ratna Asmah Susidarti Ratna Dewi Kusumaningtyas Refson, Refson Reni Nurjasmi, Reni Retno Wahyuningrum Rias Gesang Kinanti Roni Mohammad Ruslin Hadanu Rustan Hatib S Mursiti Sabirin Matsjeh Sarwo Edy Setyawan A, Setyawan Shinta Ayu Lestari Sidiq, Sidiq Siti Romlah Sja'bani, Mochammad Soedjono Aswin Sri Handayani Sri Haryanti Sri Juari Santosa Sukari, Mohd. Aspollah Sukari, Mohd. Aspollah Sumarta Sunarti Sunarti Sunaryati Supriatini Supriatini, Supriatini Surjadi, Lily Marliany Susiana Candrawati Susy Yunita Prabawati Triana Kusumaningsih Wahyono Wahyono Widya Asmara Wiwiek Fatchurohmah, Wiwiek Wiwik Cahyaningrum, Wiwik Yanti Dwi Astuti, Yanti Dwi Yayi Suryo Prabandari Yayuk Dwi Rahayu, Yayuk Dwi Zulaela Zulaela, Zulaela