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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Hortikultura Indonesia Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA International Journal of Applied Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Journal of Tropical Crop Science Warta Penelitian Perhubungan Menara Perkebunan JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Tanaman Tembakau, Serat & Minyak Industri Perbal : Jurnal Pertanian Berkelanjutan
Efendi, Darda
Agronomy And Horticulture Department, Faculty Of Agriculture, Bogor Agricultural University Jl. Meranti, IPB Darmaga Campus, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Transportation

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Journal : Jurnal AgroBiogen

 1 
Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung Iswari S Dewi; Anggi Nindita; Bambang S. Purwoko; Darda Efendi
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p89-96

Abstract

Propagation through tissue culture of plantspecies with rich secondary metabolites such as Jatrophacurcas L. is difficult to obtain. However, once established, itcan be used as one of the alternatives to supply uniformpropagules. The effects of auxin and cytokinin on theregulation of de novo woody plants shoot development havebeen studied through shoot induction, differentiation anddevelopment. The objective of this research was to identifyexplant and suitable culture media for in vitro shoot inductionthrough indirect organogenesis. Factorial experimentwas arranged in a completely randomized design, replicated20 times. The first factor was explants, i.e. cotyledons andhypocotyls. The second factor was MS media containingcombination of plant growth regulator IAA (0, 0.05, and 0.1mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of theexperiment showed that the fastest callus initiation wasachieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explantswere cultured. Shoots with leaves can be induced from bothcotyledons and hypocotyls. However, hypocotyls gave moreshoots and leaves than cotyledons when cultured in MS +IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotylsand cotyledons were successfully rooted in MS mediumwithout any growth regulator.
Embriogenesis Somatik Tidak Langsung pada Tanaman Sagu (Metroxylon sagu Rottb.) Menggunakan Sistem Kultur Suspensi, Perendaman Sesaat, dan Media Padat Imron Riyadi; Darda Efendi; Bambang S. Purwoko; Djoko Santoso
Jurnal AgroBiogen Vol 12, No 1 (2016): Juni
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v12n1.2016.p37-44

Abstract

Metode kultur in vitro yang tepat akan meningkatkan efektivitas dan efisiensi pada proses penggandaan kalus dan induksiembriogenesis somatik. Penelitian ini bertujuan mengevaluasi efektivitas tiga metode kultur jaringan, yaitu sistem kultursuspensi, sistem perendaman sesaat (SPS) atau temporary immersion system (TIS), dan media padat, untuk proliferasi kalusdan pembentukan embrio somatik secara tidak langsung pada tanaman sagu “Alitir” yang berasal dari Merauke, Papua. Bahantanaman atau eksplan awal yang digunakan adalah kalus remah hasil induksi dari kultur meristem pucuk tunas anakan sagu.Kalus tersebut dikulturkan pada media Murashige dan Skoog (MS) modifikasi dengan penambahan 2,4-D 5,0–15,0 mg/l dikombinasikandengan kinetin 0,1 mg/l menggunakan ketiga metode kultur sehingga terdapat dua belas kombinasi perlakuan.Hasil penelitian menunjukkan bobot segar kalus tertinggi sebesar 12,0 g/bejana dicapai pada metode kultur suspensi denganpenambahan 2,4-D 15,0 mg/l dikombinasikan dengan kinetin 0,1 g/l. Perolehan jumlah embrio somatik tertinggi dicapai padametode kultur suspensi dengan penambahan 2,4-D 5,0 mg/l dikombinasikan dengan kinetin 0,1 g/l sebesar 384,7 buah/bejana.Daya hidup kultur sagu terbaik dan tertinggi (100%) diperoleh pada metode kultur suspensi pada semua perlakuankonsentrasi 2,4-D. Selama proses induksi embrio somatik, terjadi perubahan warna kalus dari sebagian besar kekuninganmenjadi krem dan putih-kekuningan.
Pengaruh Retardan Paklobutrazol terhadap Pertumbuhan dan Pemulihan Dua Aksesi Ubi Kayu Surya Diantina; Darda Efendi; Ika Mariska
Jurnal AgroBiogen Vol 11, No 3 (2015): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n3.2015.p95-102

Abstract

Normal growth medium is not effective for in vitro conservation due to the risk of somaclonal variation that may increase dueto short interval between subculture. Two experiments involving growth retardant paclobutrazol (PBZ) were conducted toreduce explants growth and extend subculture interval. In order to develop medium-term conservation of cassava, therecovery of plantlets after in vitro storage was also observed. Accession 433 and 450 were used in two independentexperiments. Completely Randomized Design was used with three replications. PBZ at 0, 3.4, 6.8, and 10.2 μM weresupplemented onto MS medium + arginin 100 ppm. Observation was done on shoot length, number of nodes and leaves, andnumber of white and senescence leaves. The results showed that after nine months without subculture, both cassavaaccessions showed different results in in vitro growth and their recovery. PBZ 3.4 μM performed as the best treatment inaccession 433 and 450 to reduce in vitro growth and their recovery after storage.
Co-Authors . Angela . Rudiyanto Ade Wachjar Ade Wachjar Alfia Annur Aini Azizi and Gustaaf Adolf Wattimena Andria Agusta Andria Agusta ANDRIA AGUSTA Angela, . Anneke Pesik Asti Kusriyanti Azizi, Alfia Annur Aini Azizi, Alfia Annur Aini Bambang S PURWOKO Bambang S. Purwoko Bambang S. Purwoko C Hanny Wijaya Cece Suhara dan Joko Ridho Witono Dede Robiatul Adawiyah Deden Derajat Matra Deden Sukmadjaja Dewi Sukma Dianto, Fajar Didy Sopandie DINARTY, DINY Diny Dinarti Djoko Santoso Djoko Santoso Don R LaBonte Dorly Dorly Dwi Utami Nur Usmani Dyra Haryanti E. Gunawan Edi Santosa Entit Hermawan Erwin Al-Hafiizh Evan Maulana Fajar Dianto Fajarudin, A Fitri Fatma Wardani Fitri Fatma Wardani Furqoni, Hafith Gunawan, E. Gustaaf A Wattimena Hanifah Muthmainnah heliyana hermawati Ika Mariska Ika Roostika IKA ROOSTIKA Ika Roostika Ika Roostika Ika Roostika Ika Roostika Tambunan Imanullah Dacholfany Imron Riyadi Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iswari S Dewi Joko Ridho Witono Jollanda Effendy Jollanda Effendy Kasutjianingati . Katerin Ninariyani Ketty Suketi Kusriyanti, Asti Laela Sari laela Sari, laela Lisnandar, Dea Silvia Lolliani MASKROMO, ISMAIL Maulana, Mohamad Akhbar Maya Melati Mayasari Yamin Mohamad Akhbar Maulana Mutiara Utami Naimatul Farida Ninariyani, Katerin Nindita, Anggi NOVARIANTO, HENGKY Nurul Khumaida Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Ogie Satriadi Putra, Mirza R Rahmat Budiarto Rahmi Fajri RARA PUSPITA DEWI LIMA WATI RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Reflinur Reflinur Resa Sri Rahayu Resa Sri Rahayu Riry Prihatini ROEDHY POERWANTO Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto S Noorrohmah Satriadi, Ogie Sedyo Harsono SEDYO HARTONO Slamet Susanto Sobir Sobir Soekisman Tjitrosemito Sony Hartono Wijaya SRI HENDRASTUTI HIDAYAT Sri Yuliani Sri Yuliani Sri Yuliani Sudarsono Sukma, Dewi Sulassih, . Surya Diantina Susetio, Muhammad Tambunan, Ika Roostika Tambunan, Ika Roostika Tanari, Yulinda Taruna Shafa Arzam TENDA, ELSJE T. Tiara, Dede TRI ASMIRA DAMAYANTI Tri Istianingsih Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Triokoesoemaningtyas Triokoesoemaningtyas Vandra Kurniawan Wachjar, Ade Wahyu Fikrinda Wida W. Khumaero Willy Bayuardi Suwarno Winarso D. Widodo Yamin, Mayasari Yande Artha Gautama Yosi Zendra Joni Yosi Zendra Joni Yundari, Yundari