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SEDYO HARTONO
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Multiplex RT-PCR Assay for Crinivirus Detection Using RNA Prepared from Three Extraction Methods on Tomato Plant Esti Prasetya Ningrum; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.36558

Abstract

Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV) are members of the Crinivirus genus that induces yellowing symptoms in tomato plants. Detection of both viruses is generally carried out singly, thus it is necessary to develop a fast, accurate and efficient detection method to detect multiple viruses simultaneously in an effort to determine the suitable disease management strategies. This study was aimed to detect both viruses using the multiplex RT-PCR method and evaluate three methods of total RNA preparation used from tomato plants as RT-PCR templates. The methods evaluated were simple direct tube (SDT), simple dsRNA, and commercial kit (RNeasy Plant Mini Kit) as a comparison. The total source of RNA came from Crinivirus symptomatic tomato leaves from Kopeng, and Ketep (Central Java); Pakem (Yogyakarta); Malang (East Java); and Bogor (West Java). Single RT-PCR and multiplex RT-PCR using specific primers CPd I/CPd II and ToCV CF/ToCV CR with DNA band targets of 760 bp and 360 bp. The SDT and dsRNA methods have been successful in obtaining total RNA and viral RNA from tomato leaf samples. Total RNA RT-PCR with simple SDT and dsRNA methods followed by multiplex RT-PCR produces specific DNA band intensities that are comparable to Kit. RNA preparation with SDT and simple dsRNA methods is a simple, fast, easy and affordable method in providing templates for RT-PCR. Multiplex RT-PCR technique using two pairs of specific primers CPd I/CPd II and ToCV CF/ToCV CR is suitable for simultaneous testing of TICV and ToCV.
Molecular Characterization of Betasatellite Associated with Begomovirus on Ageratum conyzoides in Magelang, Central Java Argawi Kandito; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.46579

Abstract

Ageratum conyzoides is one of the common weeds in Indonesia. The presence of weed can decrease plant yield by resource competition and its role as alternative hosts for pests and diseases, especially begomoviruses transmitted by whitefly. The aim of this research is to detect and characterize the begomovirus-beta satellite in A. conyzoides. A. conyzoides showed severe yellowing symptoms were collected from Magelang, Central Java, Indonesia. Total DNA was extracted and analyzed using PCR method for begomovirus and beta satellite. Begomovirus detection was performed with universal primer Krusty-Homer, resulted a 500 bp DNA fragment. Betasatelit detection performed with specific primer β01/β02, resulted a 1300 bp DNA fragment, indicated presence of a beta satellite associated with the begomovirus. The sequence of begomovirus showed 95% similarity with Tomato Leaf Curl Java Virus (ToLCJaV). The sequence of beta satellites showed 85% similarity with Tomato Leaf Curl Betasatellite (ToLCB). Characterization of beta satellite showed three main characters of beta satellite, TAATATTAC stem-loop structure, Adenine-rich region, and 118 amino acids of putative ORF BC1. 
Molecular Detections and Resistance Response of Six Rice Varieties to Tungroviruses from South Sulawesi Saipul Abbas; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47355

Abstract

The suspected rice virus is found in the field, namely the tungrovirus which is transmitted by green leafhoppers (Nephotettix virescens). The study aimed to detect the tungrovirus molecularly and examine the resistance response of six rice varieties from the transmission of tungrovirus samples from South Sulawesi on a greenhouse scale. Based on the results of molecular detection with RTSV PCR of the double infected sample with DNA bands 1115 bp and RTBV of around 430 bp, Sidrap, and Maros samples were infected by 430 bp size RTBV, while Wajo sample was not detected by both viruses. The results of RTBV sequence analysis showed that the grouping of Sidrap was still one group with Maros and Pinrang samples and different from the group of samples from Malaysia, Thailand, and Philippines. While the grouping of RTSV shows that Pinrang samples are still one group with samples from Bali, Subang, and different from those of the Philippines, India, and Malaysia. The results of transmission in the greenhouse on six rice varieties (TN1, Ciherang, Mekongga, Tukad Unda, Inpari 36, Inpari 37) showed different plant resistance responses such as susceptible, moderately resistant, and resistant reactions based on the amount of disease intensity caused. Varieties that are classified as susceptible are TN1 and Ciherang varieties, moderately resistant, namely Mekongga and Tukad Unda varieties, and resistant varieties namely Inpari 36 and Inpari 37 varieties.
KOMBINASI TERMOTERAPI DAN KHEMOTERAPI DENGAN KULTUR APEKS DAN MERISTEM UNTUK ELIMINASI VIRUS MOSAIK PADA TEBU / The Combined Treatment of Thermotherapy and Chemotherapy with Apex and Meristem Culture for Mosaic Virus Elimination in Sugarcane Ika Roostika; SEDYO HARTONO; DARDA EFENDI
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.19-28

Abstract

There are several ways to eliminate virus, suach as the application of thermoterapi and chemotherapy technique, and also the apex and meristem culture. One way to control this disease is the use of virus-free seedlings. The objective of this study was to find out the effect of combined treatment between thermotherapy or chemotherapy with apex or meristem culture to mosaic virus elimination of sugarcane. There were four steps in this research: (1) Virus detection of mother plant, (2) Application of thermotherapy at 50oC and chemotherapy by using Ribavirin 25 µg/l combined with apex culture, (3) Application of thermotherapy and chemotherapy combined with meristem culture, and (4) Evaluation of virus elimination. The plant materials used were PS862 from Cirebon (PS862-Crb), PS881 from Surabaya (PS881-Sby) and PSJK922 (PSJK922-Bgr) from Bogor. Virus detection was conducted by TEM and RT-PCR analysis. The temperature for thermotherapy was 500C and the antiviral agent was Ribavirin (0 and 25 μg/l). The result showed that thermotherapy or chemotherapy combined with apex culture could not eliminate virus infection. The combined treatment of chemotherapy and meristem culture could eliminate SCSMV in variety PS862-Crb based on RT-PCR assay, however TEM analysis still detected the viral particle. It was suggessted to udertake virus indexing of large number of samples to see the rate of virus elimination.Keywords: Saccharum officinarum L., Ribavirin, Potyvirus, TEM, RT- PCR
Survey and Detection of Pectobacterium atrosepticum in Major Potato-Growing Areas in Central Java Province, Indonesia Ismiyatuningsih Ismiyatuningsih; Tri Joko; Sedyo Hartono
Jurnal Ilmu Pertanian Vol 1, No 1 (2016): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.11654

Abstract

Potato (Solanum tuberosum) is a seasonal shrub-tuber crop originated from sub-tropical area. Soft-rot is one of the most important diseases of potato. It can be caused by Pectobactorium atrosepticum, a pathogen within a status of quarantine plant pest A1 type I in Indonesia. The objective of this study was to know the incidence of potato soft rot disease and to detect P. atrosepticum in major potato-growing areas in Central Java Province by applying the serology method using DAS-ELISA technique. Survey of soft rot disease was carried out in some regencies in Central Java Province, i.e. Magelang, Banjarnegara, Wonosobo and Karanganyar. The field survey of potato plant in all the regencies indicated symptoms of stem rot which was black in color (blackleg) and foul-smelling, with disease incidence of about 10–90%. The laboratory testing showed that by applying DAS-ELISA method, P. atrosepticum was detected in samples collected from Pandean and Bagongan villages, district of Ngablak,Regency of Magelang, Central Java Province.
Identification of Important Fungal Diseases of Strawberry in Purbalingga Regency, Central Java, Indonesia Didit Setiyawan; Sedyo Hartono; Ani Widiastuti
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.145-156

Abstract

Salah satu kendala utama dalam budi daya tanaman stroberi di Indonesia adalah banyaknya penyakit tanaman. Penelitian ini merupakan lanjutan dari laporan tentang penyakit penting pada buah stroberi (Fragaria ananassa) di Kabupaten Purbalingga, Jawa Tengah untuk mengidentifikasi penyakit oleh cendawan pada bagian tanaman selain buah. Sampel berupa daun atau tanaman bergejala, diidentifikasi secara morfologi atau molekuler apabila identifikasi morfologi tidak memungkinkan. Konfirmasi gejala penyakit yang disebabkan oleh cendawan dilakukan dengan mengikuti postulat Koch, yaitu menginokulasikan cendawan patogen hasil isolasi pada tanaman stroberi sehat. Hasil penelitian menunjukkan bahwa penyakit penting yang disebabkan oleh cendawan pada tanaman stroberi adalah penyakit hawar daun yang disebabkan oleh Phomopsis sp. dan Pestalotia sp.; penyakit gosong daun yang disebabkan oleh Curvularia sp. dan Diplocarpon sp.; penyakit bercak daun yang disebabkan oleh Cercospora sp., Phoma sp., dan Gnomonia sp.; serta penyakit layu yang disebabkan oleh Verticillium sp. dan Fusarium sp. Selain laporan tentang penyakit penting tanaman stroberi, ini juga merupakan laporan pertama tentang penyakit bercak Gnomonia pada tanaman stroberi di Indonesia.
Recombinant antibody production by cloning of Pepper yellow leaf curl Indonesia virus (PepYLCIV) coat protein gene Yashanti Berlinda Paradisa; Sri Sulandari; Sedyo Hartono; Susamto Somowiyarjo; Mery Windarningsih; Dini Wahyu Kartika Sari; Christina Retna Handayani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.1221-13

Abstract

Pepper yellow leaf curl Indonesia virus (PepYLCIV) is an important pathogen on chili cultivation and is transmitted through the seed. Serological tests are sensitive, accurate, efficient and it has been widely used for the detection of seed-transmitted plant viruses. This study aimed to produce PepYLCIV recombinant protein as a material to produce recombinant antibodies PepYLCIV. DNA was extracted from infected chili leaves collected from Congkrang, Muntilan, Central Java verified using primer PepYLCIV-BamHI and PepYLCIV-EcoRI and produced an amplicon at 840 bp. The amplified fragments were cloned into the pET32a then transformed to Escherichia coli BL21. The percentage of nucleotide sequence identity and sequence of amino acid, PepYLCIV CK-6 isolates had the highest similarity of nucleotide and amino acid sequences to of chili isolates from Bandung. The expressed recombinant protein was obtained with IPTG concentration 0,5 mM and harvested at 6 hours after IPTG induction. SDS PAGE analysis of the recombinant plasmid Begomovirus CK-6 showed that the coat protein size was about 29 kDa. Immunization was carried out on rabbits by injecting 150 µg of recombinant protein 4 times with an interval of 1 week to produce crude antiserum and pure antiserum capable of detecting PepYLCIV in chili and Ageratum conyzoides using I-ELISA and DIBA tests.
Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes Muhammad Taufik; Gusnawaty HS; Syair Syair; Rahayu Mallarangeng; Andi Khaeruni; Muhammad Botek; Sedyo Hartono; Noor Aidawati; Purnama Hidayat
Jurnal Fitopatologi Indonesia Vol 19 No 3 (2023)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.3.89-98

Abstract

Penyakit daun keriting kuning pada pertanaman cabai di Sulawesi Tenggara telah dilaporkan sejak tahun 2018, yaitu di Kabupaten Kolaka Timur dan Kota Kendari. Gejala penyakit di lapangan semakin meluas seiring dengan perluasan penanaman cabai. Penelitian bertujuan menghitung kembali insidensi penyakit daun keriting kuning pada pertanaman cabai di Sulawesi Tenggara, mengidentifikasi serangga yang berasosiasi dengan tanaman cabai, dan mengidentifikasi penyebab penyakitnya. Pengamatan insidensi penyakit dilakukan di pertanaman cabai yang berada di Kabupaten Bombana, Konawe Selatan, Konawe, Kendari, Kolaka Timur, Kolaka, dan Kolaka Utara. Identifikasi kutukebul dilakukan berdasarkan karakter morfologi. Deteksi dan identifikasi begomovirus menggunakan metode polymerase chain reaction, yang dilanjutkan dengan analisis sikuensing. Rata-rata insidensi penyakit daun keriting kuning di tujuh kabupaten ialah 36%–90%. Spesies kutukebul yang ditemukan adalah Aleurotrachelus trachoides. Fragmen DNA spesifik begomovirus berukuran 580 pb berhasil diamplifikasi dari sampel tanaman cabai asal tujuh kabupaten di Sulawesi Tenggara. Analisis sikuen mengonfirmasi infeksi Pepper yellow leaf curl Indonesia virus pada pertanaman cabai di Kabupaten Kolaka, Kolaka Utara, Bombana, Konawe, dan Konawe Selatan.
POTENTIAL EFFECT OF RHIZOBACTERIA Streptomyces sp. AND Bacillus sp. TO GEMINIVIRUS INFECTION AND RED CHILI (Capsicum annum L.) QUALITY PRODUCTION Azizah Ridha Ulilalbab; Sri Sulandari; Triwidodo Arwiyanto; Sedyo Hartono
Jurnal Agroteknologi Vol 14, No 1 (2023): Agustus 2023
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v14i1.24689

Abstract

The chili yellow leaf curl disease is one of the biggest obctacle in chili’s production in Indonesia. This diseases only transmitted by whitefly (Bemisia tabaci). One of the disease controlling method is by using Plant Growth Promoting Rhizobacteria (PGPR) to reduce pesticide application for the health and environment. The aim of this research is to find out the effectiveness of Streptomyces sp. and Bacillus sp. in supressing yellow leaf curl disease, plant growth and chili harvest. This research has conducted at the experimental farmer’s field in Harjobinangun, Pakem, Sleman, Special Region of Yogyakarta. Using varieties of chili Twist 42, Streptomyces isolates which from Bacteriology Laboratory collection’s with 10-8 CFU/ml and Bacillus isolates which is from Mycrobiology Laboratory collection with  10-8 CFU/ml. The result of this research showed that the treatment of combination between Bacillus sp. and Streptomyces sp. could decrease and supress disease incidence and disease intensity if it compared with others treatment. Parameters of plant growth included plant high, stem diameter, root length, root volume and number of productive branch showed that single of Streptomyces sp. and Bacillus sp. without combination were better than combination and untreated.  The single application Streptomyces sp. or Bacillus sp. could increase amount of total number and weight of fruits.
Virus Gemini Laporan pertama infeksi virus gemini pada tanaman tomat di Sulawesi Tenggara Muhammad Taufik; Gusnawaty HS; Asmar Hasan; La Ode Santiaji Bande; Siti Anima Hisein; Hasdiana Hasdiana; Nur Isnaini Ulfa; Sedyo Hartono
Agrokompleks Vol 24 No 1 (2024): Agrokompleks Edisi Januari
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v24i1.730

Abstract

Begomovirus termasuk genus dari famili Geminiviridae juga dikenal dengan nama Geminivirus. Geminivirus adalah penyebab penyakit pada beberapa komoditas sayuran termasuk tomat. Infeksi Geminivirus dapat menyebabkan kerugian berkisar 85% bahkan gagal panen, khususnya infeksi terjadi pada tanaman muda. Penularan Geminivirus di pertanaman dimediasi oleh serangga vektor kutukebul Bemisia tabaci (Hemiptera:Aleyrodidae). Tujuan penelitian adalah mengidentifikasi infeksi Geminivirus dan serangga vektor pada pertanaman tomat. Lokasi pengamatan di Desa Wolasi dan Lamomea, Kecamatan Konda, Kabupaten Konawe Selatan. Sampel daun tomat yang bergejala Geminivirus dimasukkan ke dalam kantong plastik sampel ziplock yang telah diisi CaCl2, kemudian dimasukkan di dalam kotak pendingin. Sampel tomat yang bergejala Geminivirus dideteksi dengan teknik polymerase chain reaction (PCR) di Laboratorium Virologi, Faperta Universitas Gadjah Mada. Primer yang digunakan adalah primer universal Geminivirus pAV494 dan pAC1048. Gejala Geminivirus yang ditemukan pada tanaman tomat adalah mosaik ringan atau menguning, daun mengecil, malformasi daun, daun agak menggulung ke atas, dan tulang daun mengalami penebalan. Serangga vektor kutukebul (Bemisia tabaci) dan kelompok telur hampir selalu ditemukan di pertanaman tomat. Teknik PCR berhasil mengamplifikasi DNA Geminivirus yang berukuran 500bp. Kejadian penyakit Geminivirus pada tanaman tomat di Desa Wolasi dan Lamomea adalah 40% dan 34%, secara berturut turut. Penelitian ini telah mengonfirmasi keberadaan infeksi Geminivirus yang pertama kali pada pertanaman tomat di Sulawesi Tenggara.
Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika Andi Khaeruni Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Budi Setiadi Daryono Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika Gusnawaty HS GUSNAWATY HS, GUSNAWATY Hasdiana Hasdiana Helina, Selvi Heri Widarta I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Botek Muhammad Muhsin MUHAMMAD TAUFIK Muhammad Taufik Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nur Isnaini Ulfa Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng Rahma Ayu Priani Resti Fajarfika Retno Mastuti Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siti Anima Hisein Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono