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All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Jurnal Penelitian Tanaman Industri (Littri) Agrokompleks

SEDYO HARTONO

Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281

Author-ID : 3094341

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Propagation and Purification of Baculovirus oryctes Huger Susamto Somowiyarjo; YB Sumardiyono; Sedyo Hartono; Triharso Triharso; Jun Kobayashi
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

An isolate of Baculovirus oryctes, a possible biological control agent for coconut beetle (Oryctes rhinoceros Huger) from East Java was propagated and purified. The virus could be transmitted by feeding the imago with 10% sucrose containing virus from homogenate of infected beetles. Effectivity of virus to 9 healthy females by sexual copulation. Virus be succesfully purified by a method of Payne.

Optimasi Metode PCR untuk Deteksi Pectobacterium carotovorum, Penyebab Penyakit Busuk Lunak Anggrek Tri Joko; Nanda Kusumandari; Sedyo Hartono
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Soft rot is one of the most important diseases of orchids caused by Pectobacterium carotovorum. The conventional methods for the detection of pathogen is tedious and time consuming. In recent years, numerous molecular diagnostic approaches for the detection of P. carotovorum have been developed, including various PCR-based assays. Optimization of PCR technique to DNA amplification is essential for time and material efficiency, which will make detection to be rapid and more appropriate. The purposes of this study were to decide concentration of DNA and primer, and also the concentration of bacterial pure cultures and primer to amplify 16S rRNA gene fragment. Optimization of PCR was done by using various concentration of DNA, pure cultures of bacteria, and primer to amplify the 16S rRNA gene sequence. The results showed that the most optimum concentration to amplify 16S rRNA gene sequence at DNA and primer concentration were 63,4 ng/µl and 10 pmol, while pure cultures and primer concentrations were at 8×109 CF U/ml and 10 pmol respectively. Penyakit busuk lunak yang disebabkan oleh Pectobacterium carotovorum merupakan salah satu penyakit penting pada tanaman anggrek. Deteksi patogen secara cepat dan akurat dapat dilakukan secara molekular menggunakan teknik Polymerase chain reaction (PCR). Optimasi metode PCR perlu dilakukan untuk mengefisienkan waktu dan penggunaan bahan sehingga proses deteksi dapat dilakukan dengan cepat dan tepat. Penelitian ini bertujuan untuk menentukan konsentrasi DNA dengan primer maupun konsentrasi kultur murni bakteri dengan primer yang paling tepat untuk mendapatkan fragmen gen 16S rRNA. Optimasi PCR dilakukan menggunakan beberapa variasi pengenceran pada DNA, kultur murni bakteri, dan primer untuk mengamplifikasi gen 16S rRNA. Hasil penelitian menunjukkan bahwa konsentrasi yang paling optimal untuk mengamplifikasi gen 16S rRNA yaitu DNA dan primer masing-masing sebesar 63,4 ng/µl dan 10 pmol, sedangkan konsentrasi kultur murni dan primer sebesar 8×109 CFU/ml dan 10 pmol.

Pengaruh Tinopal terhadap Patogenisitas Nucleopolyhedrovirus pada Spodoptera litura Ma'unah Ambarwati; Arman Wijonarko; Sedyo Hartono
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11731

Susceptibility of the armyworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) to nucleopolyhedrovirus was evaluated using droplet feeding methods. S. litura was originally collected from the field in Bantul and has been reared continuously in the laboratory using artificial diet. The tested instars were exposed a series concentration of nucleopolyhedrovirus (2×103, 2×104, 2×105, 2×106, 2×107, 2×108, 2×109 PIB/ml) which were added with Tinopal 0,5% and 1%. The result indicated that the larval mortality of 3rd, 4th, and 5th instars Tinopal, significantly different with the addition of 1% Tinopal. This addition increased the effectiveness of NPV for 235, 25117, and 6.6 million fold. The observation of the larval midgut which was treated by Tinopal, showed that Tinopal physically disrupt the peritrophic membrane. Therefore, it can be suggested that the Tinopal facilitates the entry of NPV to the host insect.

Deteksi dan Diferensiasi Virus Kerdil Pisang dengan Teknik PCR-RFLP Rahma Ayu Priani; Susamto Somowiyarjo; Sedyo Hartono; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11736

Banana bunchy top disease (BBTD) can be caused by the infection of two different viruses, Banana bunchy top virus (BBTV) or Abaca bunchy top virus (ABTV). Both viruses can be transmitted persistently by aphid Pentalonia nigronervosa Coq. The research was conducted to detect and to differentiate the virus bypolymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) techniques. Infected plants were collected from Yogyakarta (Sleman, Yogyakarta city, Bantul, Gunung Kidul, and KulonProgo). Nucleon Phytopure DNA Extraction Kit method was used to extract the total DNA of infected plants. Universal primers of Common DNA region (S-CRF and S-CRR) and specific primers DNA-R (C1-CRF and CI-CRR) were used for PCR amplification. PCR products were analyzed by RFLP technique using the restriction enzyme of DraI. The results reconfirm previous reports that bunchy top disease of banana in Yogyakarta is caused by BBTV. The ABTV was not detected in this present study. Based on the RFLP analysis it was concluded that BBTV collected in this study could be divided into three groups. Group 1 consisted of BBTV isolate from Sleman and Yogyakarta city with two fragments DNA of 400 and 388 bp. Group 2 consisted of isolate BBTV from Kulon Progo and Gunung Kidul with three fragments DNA of 400, 388, and 323 bp. Group 3 consisted of isolate from Bantul with two fragments DNA of 723 and 376 bp. Further study on the complete characteristics of these groups is still needed.

Deteksi Keragaman Virus Tungro dari Beberapa Daerah Endemis di Indonesia dengan Teknik PCR-RFLP R. Heru Praptana; Y. B. Sumardiyono; Sedyo Hartono; I Nyoman Widiarta; Muhammad Muhsin
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11763

Tungro is one of rice disease caused by two different viruses (rice tungro virus=RTV) i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) that are transmitted only by green leafhopper. Tungro had become a serious problem in several rice productions centre in Indonesia. Various components of management effort have been applied but they were inefficient in preventing the tungro disease development. Resistance variety is the most efficient component to tungro disease management. Complexity interactions of tungro disease components are mayor constraint in tungro disease management. Detection of molecular variability in rice tungro virus from several endemic areas in Indonesia were conducted by using PCR-RFLP technique. Existence of RTBV and RTSV in the infected plants collected from several endemic areas were successfully detected by PCR. The RFLP analysis with restriction enzymes BstYI and HindIII showed that there were significant difference among the RTSV originated from Java, Bali and Sulawesi.

Characterization of A Baculovirus of Spodoptera litura (Lepidoptera : Noctuidae) Isolated from Yogyakarta Arman Wijonarko; Sedyo Hartono; Tri Harjaka; Nuri Yusmarlita
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11813

A Baculovirus has been isolated from cadaver of larvae of Spodoptera litura, a Noctuidae of agricultural pest, importance due to its wide-range hosts and the damage to their respective host. Phase contrast light microscopy observation from infected larvae showed that the fat body, hemocyte cells, and cells surrounding the trachea or tracheolus were the most tissue invaded by polyhedra. Transmission electron microscopy analysis of the occlusion body purified from diseased larva showed that the baculovirus envelope containing multiple nucleocapsid. Digestion of viral DNA with three restriction enzymes showed that the genome pattern of baculovirus isolated from Bantul were close to SpliNPV isolated from Japan and those of Spodoptera littoralis and quite distinct from those isolated from Southeast Asia region. Bioassay test performed on first to fifth instar larvae showed that the virus effectively control the young larvae, but showed some level of resistance against older larvae of Spodoptera litura.

Observasi dan Identifikasi Virus yang Menginfeksi Bawang Merah di Jawa Tuty Arisuryanti; Budi Setiadi Daryono; Sedyo Hartono; Anak Agung Gde Raka Swastika
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11883

This study was conducted to observe and identify viruses from infected shallots in several shallot planting center. The observation was done in eight areas of three provinces (Yogyakarta, Central Java, and East Java). Leaves from shallot plants and shallot germination showing virus symptoms were examined. The leaves were then investigated to identify viruses infecting shallots using Enzyme-Linked Immunosorbent Assay (ELISA). The result revealed that the type of virus symptoms infecting the shallots was a mozaic symptom with yellow strips. The ELISA analysis showed that Tawangmangu Biru shallot cultivar plants sampled from Blumbang, Tawangmangu (Central Java) and Philiphine Bima shallot cultivar seeds collected from Srigading, Sanden, Bantul (Yogyakarta) were positively infected by Onion Yellow Dwarf Virus (OYDV). The result also revealed that Biru, Kuning Tablet, Lokal Tawangmangu, and Bima Curut shallot cultivars had the potency to be virus resistant plants and could be considered as candidates for breeding program.

Pemurnian dan Deteksi Serologi Patchouli Mottle Virus pada Tanaman Nilam Sedyo Hartono; Siti Subandiyah; Aminatun Munawarti; Retno Mastuti; Serafinah Indriani
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12044

Patchouli mottle virus (PatMoV) is the most severe disease pathogen and causes substantial losses in many patchouli-producing regions in Indonesia. Serological detection tool for the disease was developed in this research. Virus isolation was conducted on Chenopodium amaraticolor resulted on the homogenous local lesions 6 days after inoculation. Virus purification was obtained from 200g inoculated leaves resulted on 2 ml virus solution with the concentration of 1 mg/ml. Polyclonal antibodies were produced on rabbits. Harvested antiserum was used for further virus detection by Indirect-Enzyme linked Immunosorbent assay (I-ELISA) and dot-immunobanding assay (DIBA) techniques. The antibodies were positively reacted with purified viruses, infected field collection of patchouli, and inoculated C. amaranticolor. On the other hand un-inoculated C. amaranticolor samples and healthy patchouli generated from tissue cultures gave negative reaction with the antibodies. This is the first report of cheap practical antibody production for PatMoV detection in Indonesia.

Epidemi Penyakit Daun Keriting Kuning Cabai Y. B. Sumardiyono; Sedyo Hartono; Sri Sulandari
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12269

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Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Fariha Wilisiani; Susamto Somowiyarjo; Sedyo Hartono
Jurnal Perlindungan Tanaman Indonesia Vol 18, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.15602

One of important problems of melon farming in Yogyakarta is a leaf curl disease that show specific symptom of Begomovirus infection. The little information about the nature of virus causal agent was constraint for the disease management. The purposes of this research were to molecularly identify the leaf curl causing virus in melon in Yogyakarta and to find the genetic relationship of this virus with other Begomovirus members which cause leaf curl disease. This research was conducted through several phases, which are: curly leaves collection on the field, virus DNA isolation, Begomovirus identification using universal primer Krusty Homer, Begomovirus DNA-A and DNA-B detection using primer Gemini full BamH1 forward and reverse for full genome DNA-A, and primer BF518 and BR1641 for DNA-B, genome sequencing and genetic relationship analysis of the sequence with other Begomovirus causing leaf curl. The result of field studies which were conducted in Sewon found some melon plant with symptom of Begomovirus infection. The molecular identification result using PCR showed that leaf curl causing virus in melon is Begomovirus, having DNA-A and DNA-B. Genetic relationship analysis of this virus with other Begomovirus causing leaf curl shows that this virus is closely related with Pepper yellow leaf curl Indonesia virus (AB267834) based on nucleotide and amino acid sequencing as coat protein of Begomovirus. The result of shows that the study is the first report of PepYLCIDV infection, a bipartite genome virus on melon, and its natural leaf curl symptom in Indonesia. Salah satu kendala budidaya melon (Cucumis melo L.) di Yogyakarta yaitu adanya penyakit daun keriting dengan gejala khas infeksi Begomovirus. Belum tersedianya informasi mengenai jenis dan ciri patogen virus penyebab penyakit tersebut merupakan salah satu kendala penting dalam menentukan strategi pengelolaan virus tersebut. Tujuan penelitian ini adalah mengidentifikasi secara molekuler virus penyebab daun keriting pada melon di Yogyakarta dan mengetahui hubungan kekerabatan virus tersebut dengan virus anggota Begomovirus lain penyebab daun keriting yang telah dipublikasi di database genebank. Penelitian dilakukan dengan beberapa tahapan, yaitu deskripsi gejala daun keriting di lapangan, isolasi DNA virus, identifikasi Begomovirus dengan primer universal Krusty Homer, deteksi DNA-A dan DNA-B Begomovirus dengan primer Gemini full BamH1 forward dan reverse untuk full genome DNA-A, serta primer BF518 dan BR1641 untuk DNA-B, sequencing genom, dan analisis hubungan kekerabatan sequence tersebut dengan Begomovirus lain penyebab daun keriting. Hasil pengamatan lapangan di Sewon Bantul diperoleh tanaman melon dengan gejala khas infeksi Begomovirus. Hasil identifikasi secara molekuler dengan PCR menunjukkan bahwa virus penyebab daun keriting pada melon adalah Begomovirus, memiliki DNA-A dan DNA-B. Analisis hubungan kekerabatan virus penyebab daun keriting pada melon dengan Begomovirus lain penyebab daun keriting menunjukkan bahwa virus tersebut berkerabat dekat dengan Pepper yellow leaf curl Indonesia virus (AB267834) berdasarkan sekuen nukleotida dan asam amino sebagian coat protein Begomovirus. Hasil penelitian ini menunjukkan bahwa penelitian ini merupakan laporan pertama infeksi PepYLCIDV dengan bipartite genome pada melon dengan gejala daun keriting secara alamiah di Indonesia.

Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika Andi Khaeruni Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Budi Setiadi Daryono Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika Gusnawaty HS GUSNAWATY HS, GUSNAWATY Hasdiana Hasdiana Helina, Selvi Heri Widarta I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Botek Muhammad Muhsin MUHAMMAD TAUFIK Muhammad Taufik Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nur Isnaini Ulfa Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng Rahma Ayu Priani Resti Fajarfika Retno Mastuti Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siti Anima Hisein Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono

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Journal : Jurnal Perlindungan Tanaman Indonesia