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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Hortikultura Indonesia (JHI) Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA International Journal of Applied Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Journal of Tropical Crop Science Warta Penelitian Perhubungan Menara Perkebunan JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Tanaman Tembakau, Serat & Minyak Industri Perbal : Jurnal Pertanian Berkelanjutan
Efendi, Darda
Agronomy And Horticulture Department, Faculty Of Agriculture, Bogor Agricultural University Jl. Meranti, IPB Darmaga Campus, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Transportation

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Journal : Jurnal Bioteknologi

 1 
DETEKSI VARIASI SOMAKLONAL PLANLET Jatropha curcas Linn. HASIL REGENERASI EMBRIO SOMATIK DENGAN MARKA MOLEKULAR ISSR . Rudiyanto; Darda Efendi; Erwin Al-Hafiizh; Tri Muji Ermayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.871 KB) | DOI: 10.29122/jbbi.v8i1.4092

Abstract

Physic nut (Jatropha curcas Linn.) has the potential as a source of sustainable biofuels. Somatic embryo proliferation of J. curcas may cause somaclonal variations. This research aimed to investigate somaclonal variations of J. curcas somatic embryo derived-plantlet using ISSR markers. Somatic embryos of J. curcas at the globular phase were cultured on liquid MS medium supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg L-1 of 2,4-D. Parameter observed were embryos weight, embryos volume, colour, and size of embryos. After proliferation, the embryos were cultured on a germination medium until the cotyledonary phase. The results showed that proliferation of J. curcas somatic embryos was optimal, with the highest weight and volume,  at MS medium added with 1 mg L-1 2,4-D.
EVALUASI PERTUMBUHAN, KANDUNGAN KLOROFIL DAN KAROTENOID TORBANGUN (Coleus amboinicus Lour.) POLIPLOID MELALUI KULTUR IN VITRO Evan Maulana; Darda Efendi; Laela Sari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.6 KB) | DOI: 10.29122/jbbi.v8i2.4834

Abstract

Torbangun (Coleus amboinicus Lour.) is an important medical plant contains an active ingredient. The leaves contain phytochemical which influence to increase of milk production. torbangun was classified as a sterile seed thus this plant cannot be propagated by seed. The polyploid induction is one such approach that may introduce phenotypic characteristic lead to increase genetic diversity. This study aimed to evaluate the growth of torbangun polyploid explants through in vitro which cultured on Murashige and Skoog (MS) medium without PGR. The experiment used a completely randomized design with four replicates. Each replicate consisted of four explants. The parameters observed were shoot height, number of axillary shoots, leaves and roots recorded every week until the 8 weeks of culture. The results showed that torbangun polyploid (tetraploid) had higher growth (shoot height and leaves number) significantly compared to diploid (control) explants, while there were no significantly difference in the number of axillary shoots roots. The polyploid induction of torbangun enhanced the chlorophyll and carotenoids content. Torbangun (Coleus amboinicus Lour) merupakan tanaman obat penting yang mengandung bahan aktif. Daunnya mengandung fitokimia yang berpengaruh terhadap peningkatan produksi susu. Torbangun tergolong benih mandul sehingga tanaman ini tidak dapat diperbanyak dengan biji. Induksi poliploid merupakan salah satu pendekatan yang dapat memperkenalkan sifat fenotipik yang dapat meningkatkan keragaman genetik. Penelitian ini bertujuan untuk mengevaluasi pertumbuhan eksplan torbangun poliploid secara in vitro yang dikultur pada media Murashige dan Skoog (MS) tanpa ZPT. Percobaan menggunakan rancangan acak lengkap dengan empat ulangan. Setiap ulangan terdiri dari empat eksplan. Parameter yang diamati adalah tinggi pucuk, jumlah tunas ketiak, daun dan akar dicatat setiap minggu sampai 8 minggu kultur. Hasil penelitian menunjukkan bahwa poliploid (tetraploid) torbangun memiliki perbedaan pertumbuhan (tinggi tunas dan jumlah daun) yang lebih tinggi dibandingkan dengan eksplan diploid (kontrol), sedangkan jumlah tunas ketiak akar tidak berbeda nyata. Induksi poliploid torbangun meningkatkan kandungan klorofil dan karotenoid.
Co-Authors . Angela Ade Wachjar Ade Wachjar Alfia Annur Aini Azizi and Gustaaf Adolf Wattimena Andria Agusta ANDRIA AGUSTA Andria Agusta Angela, . Anneke Pesik Asti Kusriyanti Azizi, Alfia Annur Aini Azizi, Alfia Annur Aini Bambang S PURWOKO Bambang S. Purwoko Bambang S. Purwoko C Hanny Wijaya Cece Suhara dan Joko Ridho Witono Dede Robiatul Adawiyah Deden Derajat Matra Deden Sukmadjaja Dewi Sukma Dianto, Fajar Didy Sopandie DINARTY, DINY Diny Dinarti Djoko Santoso Djoko Santoso Don R LaBonte Dorly Dorly Dwi Utami Nur Usmani Dyra Haryanti E. Gunawan Edi Santosa Entit Hermawan Erwin Al-Hafiizh Evan Maulana Fajar Dianto Fajarudin, A Fitri Fatma Wardani Fitri Fatma Wardani Furqoni, Hafith Gunawan, E. Gustaaf A Wattimena Hanifah Muthmainnah heliyana hermawati Ika Mariska Ika Roostika IKA ROOSTIKA Ika Roostika Ika Roostika Ika Roostika Ika Roostika Tambunan Imanullah Dacholfany Imron Riyadi Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iswari S Dewi Joko Ridho Witono Jollanda Effendy Jollanda Effendy Kasutjianingati . Katerin Ninariyani Ketty Suketi Kusriyanti, Asti Laela Sari laela Sari, laela Lisnandar, Dea Silvia Lolliani MASKROMO, ISMAIL Maulana, Mohamad Akhbar Maya Melati Mayasari Yamin Mohamad Akhbar Maulana Mutiara Utami Naimatul Farida Ninariyani, Katerin Nindita, Anggi NOVARIANTO, HENGKY Nurul Khumaida Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Ogie Satriadi Putra, Mirza R Rahmat Budiarto Rahmi Fajri RARA PUSPITA DEWI LIMA WATI RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Reflinur Reflinur Resa Sri Rahayu Resa Sri Rahayu Riry Prihatini ROEDHY POERWANTO Rudiyanto Rudiyanto S Noorrohmah Satriadi, Ogie Sedyo Harsono SEDYO HARTONO Slamet Susanto Sobir Sobir Soekisman Tjitrosemito Sony Hartono Wijaya SRI HENDRASTUTI HIDAYAT Sri Yuliani Sri Yuliani Sri Yuliani Sudarsono Sukma, Dewi Sulassih, . Surya Diantina Susetio, Muhammad Tambunan, Ika Roostika Tambunan, Ika Roostika Tanari, Yulinda Taruna Shafa Arzam TENDA, ELSJE T. Tiara, Dede TRI ASMIRA DAMAYANTI Tri Istianingsih Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Triokoesoemaningtyas Triokoesoemaningtyas Vandra Kurniawan Wachjar, Ade Wahyu Fikrinda Wida W. Khumaero Willy Bayuardi Suwarno Winarso D. Widodo Yamin, Mayasari Yande Artha Gautama Yofita Sandra, S.Pd., M.Pd., Zico Farlin, Dr. Budiwirman, M.Pd., Yosi Zendra Joni Yosi Zendra Joni Yundari, Yundari