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All Journal HAYATI Journal of Biosciences PROSIDING SEMINAR NASIONAL Jurnal Biologi Udayana METANA Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Kesehatan Masyarakat Indonesian Journal of Mathematics and Natural Sciences Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Sainteknol : Jurnal Sains dan Teknologi Journal of Biomedicine and Translational Research Indonesian Journal of Biotechnology The Indonesian Biomedical Journal BIOMA Journal of Biology Education Innovative Journal of Curriculum and Educational Technology Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Keperawatan Silampari Biosaintifika: Journal of Biology & Biology Education Journal of Maternal and Child Health Jurnal Litbang: Media Informasi Penelitian, Pengembangan dan IPTEK Journal of Innovative Science Education Jurnal Kesehatan Unnes Journal of Public Health Public Health Perspective Journal Jurnal Berkarya Pengabdian Masyarakat Jurnal Humanities Pengabdian Kepada Masyarakat Jurnal Abdidas Jurnal Dunia Gizi Journal of Pharmaceutical and Health Research Life Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Pengabdian Masyarakat Dharma Andalas Jurnal Pengabdian Kolaborasi dan Inovasi IPTEKS Jurnal Pengabdian Masyarakat Bangsa Prosiding Seminar Nasional Pascasarjana Proceeding of International Conference on Science, Education, and Technology Prosiding Seminar Nasional Biologi
Ari Yuniastuti
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Journal : Biosaintifika: Journal of Biology

 1 
STATUS ANTIOKSIDAN GLUTATION PADA PASIEN TUBERKULOSIS PARU DI BALAI KESEHATAN PARU (BKPM) MAKASSAR Yuniastuti, Ari; Yusuf, Irawan; Massi, Muh Nasrum; -, Budu
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2745

Abstract

AbstrakAntioksidan merupakan senyawa untuk pertahanan tubuh host terhadap radikal bebas. Pada pasien tuberkulosis paru (TB paru), rendahnya antioksidan menyebabkan kerusakan jaringan. Salah satu antioksidan tubuh adalah glutation. Rendahnya glutation berhubungan dengan gangguan sistem imun, sehingga menyebabkan keparahan penyakit pada pasien TB paru. Penelitian ini bertujuan mengetahui kadar glutation plasma penderita tuberkulosis paru. Desain penelitian ini adalah kohort prospektif. Sampel TB paru dipilih di Balai Besar Kesehatan Paru Masyarakat (BBKPM), Makassar yang sesuai dengan kriteria inklusi dan eksklusi dan sesuai kadatangan (consecutive sampling). Dua mililiter darah diambil dari setiap sampel, kemudian dilakukan pemeriksaan kadar glutation dengan ELISA. Hasil penelitian menunjukkan bahwa kadar glutation sampel pasien TB paru sebelum pemberian obat antituberkulosis (OAT) sebesar 0,483830,0344 mM dan setelah pemberian OAT sebesar 0,494650,0371 mM. Setelah pemberian OAT kadar glutation mengalami peningkatan sebesar 2,2% meskipun masih lebih rendah dari kadar glutation normal.AbstractAntioxidants prevent hosts against free radicals. In pulmonary tuberculosis (TB) patients, the low level of antioxidants may cause tissue damage in oxidative hosts. One of antioxidants is glutathione. The poor glutathione level correlates with immune system disorder. The current study aimed to determine glutathione plasma levels in pulmonary tuberculosis patients. The study design was prospective cohort. Pulmonary TB samples were obtained from patients in the Lung Health Centre (BBKPM) in Makassar, and they were selected based on the inclusive and exclusive criteria and on their admittance times (a consecutive sampling technique). Two mililiters of blood was taken from each sample and the glutathione level was examined using ELISA. The study indicated that the glutathione level of the pulmonary TB patients before administered with antitubeculosis drug was 0.483830.0344 mM and after antituberculosis drug was 0.494650.0371 mM. After administration of antituberculosis drug the glutathione level increased by 2.2%, although still lower than normal glutathione level.
Efektivitas Seng (Zn) sebagai Imunostimulan dalam Produksi Reactive Oxygen Intermediate pada Mencit Balb/C yang Diinfeksi Salmonella typhimurium Yuniastuti, Ari; H, Nugrahaningsih W; Zunikhah, Zunikhah
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1151

Abstract

This study aims to prove the effectiveness of zinc as a micronutrient to increaseimmunostimulatory Reactive Oxygen Intermediate (ROI) production in balb/c miceinfected by Salmonella typhimurium. Research was designed using randomized thePost Test Only Control Group Design. The sample of 24 male mice balb/c are groupedinto four groups, i.e. one control group and three treatment groups (treatment dose of 78ppm/day; 169 ppm/day and 260 ppm/day) intraperitoneally for 14 days with each groupconsisting of six rats. The independent variable is the provision of zinc with various doses,dependent variable is the ROI production of macrophages, which are checked by usingNitroblue Tetrazolium (NBT) Reduction Assay, and control variables are body weight,gender and age. All mice infected with Salmonella typhimurium 105 intraperitoneally onday 8th. At the end of treatment mice were killed and was examined for levels of ROI.Inspection results were analyzed by Kruskal Wallis test. Result showed that doses ofzinc of 78 ppm/day have immunostimulatory effective as the highest ROI productionof mice Balb/c infected with Salmonella typhimurium.Keywords: immunostimulant, Reactive Oxygen Intermediate, zinc (Zn)
EFEK PERENDAMAN INFUSA DAUN SALAM (Syzygium polyanthum) TERHADAP KUALITAS DAGING AYAM POSTMORTEM Agustina, Feri Dwi; Widiyaningrum, Priyantini; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2271

Abstract

Tujuan penelitian ini untuk mengevaluasi efek perendaman daging ayam broiler dalam konsentrasi infusa daun salam yang berbeda. Penelitian menggunakan desain faktorial dengan 3 taraf konsentrasi infusa (0%; 5%; dan 10%) serta dua waktu pengamatan yaitu 0 dan 8 jam setelah perlakuan perendaman. Parameter yang diukur meliputi rata-rata bilangan peroksida, pH, kadar air dan water holding capacity (WHC). Data dianalisis menggunakan analisis varian dan uji perbedaan akibat perlakuan dilakukan dengan uji beda nyata terkecil. Hasil penelitian menunjukkan bahwa pengukuran pH dan WHC sesaat setelah perlakuan memperlihatkan perbedaan nyata (P<0,05), sementara bilangan peroksida dan kadar air tidak berbeda nyata. Pada 8 jam setelah perendaman, terlihat bahwa nilai peroksida, pH, kadar air dan WHC menunjukkan perbedaan nyata. Penelitian ini menyimpulkan bahwa infusa daun salam mampu memperlambat laju bilangan peroksida, memperlambat penurunan pH dan memperlambat penurunan WHC.The aim of this study was to evaluate the effects of the immersion of chicken meat in the bayleaf extract and the time of measurement on the quality of the meat. This was a factorial experiment with three levels of bayleaf extract concentrations (0%, 5% and 10%), and two time of measurement (at 0 and 8 hours after the treatment). The parameters observed were the average peroxide value, the pH, the water content and the water holding capacity. The data were analyzed using ANOVA and followed by the least significant difference test for any significant difference. The results showed that the pH and the water holding capacity were significantly different (P<0.05), whereas the peroxide value and the water content were not significantly different. Nevertheless, at eight hours after the immersion it was seen that the peroxide value, the pH, the water content and the water holding capacity were significantly different. It was concluded that the extract of bayleaves might slower the rate of the peroxide value, could decrease the pH, and decrease the water holding capacity.
PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl Susanti, R; Fibriana, Fidia; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12177

Abstract

Phylogenetic or population genetic studies have been focused on finding genetic variations in mitochondrial DNA (mtDNA) because mutations in this region contained five times higher than other genetic materials. The aim of this research was to generate PCR-RFLP D-loop mtDNA profiles of some Indonesian domestic waterfowl. The samples were 35 local ducks, 5 muscovy duck, and 5 geese. These local ducks included on seven types of Javanese ducks in Central Java, i.e. Magelang duck, Peking duck, Pengging duck, Tegal Branjangan duck, Tegal Jarakan duck, Tegal Blorong duck, and Tegal Lemahan duck. PCR technique was used to amplify the D-loop genes, and RFLP analysis of the D-loop was performed with AluIand RsaI. The results show that muscovy duck has no restriction sites in D-loop gene. Moreover, goose has a restriction site of AluI in D-loop gene at 550 bp and 187 bp; and it has no restriction site of RsaI. The analysis of RsaI-RFLP in mtDNA D-loop region of ducks resulted in 1 restriction fragment pattern which can be applied in the identification of duck to differentiate it from goose and muscovy duck. PCR-RFLP analysis duck D-loop mtDNA using AluI and RsaI restriction enzyme resulted in 3 combinations of restriction fragment pattern shown in 3 haplotypes (A, B, and C). Genetic diversity of Indonesian domestic waterfowl population could be identified by using PCR-RFLP analysis on mtDNA D-loop region. So far, there are few results of research on Indonesian domestic waterfowl genetic characteristics based on PCR-RFLP mtDNA D-loop marker. Also, this research has a good contribution on genetic characterization of local duck. The characterization is an important aspect to maintain breed quality and manage the genetic resources of Indonesian germplasm and in facilitating the breeding program.
Biosorption of Heavy Metal Pollution by Enterobacter agglomerans Dewi, Nur Kusuma; Mubarok, Ibnul; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20471

Abstract

Biosorption is a new waste treatment technology that can eliminate toxic heavy metals. Biosorption can be considered as an environmentally friendly alternative technology to treat industrial liquid waste that is economically proper to use. One of them is biosorption that utilizes the microorganisms’ absorption ability, especially bacteria that can absorb heavy metals in waters, such as Enterobacter agglomerans. This research aimed to determine the ability of E. agglomerans in reducing heavy metals pollution in local river. The research employed the measurement of the effect of lead (Pb) to E. agglomerans growth using Optical Density (OD) at wavelength 600 nm. The colony numbers were calculated using a standard curve. While the ability of E. agglomerans to reduce heavy metals concentration in liquid media was measured using AAS with a wavelength of 240 nm. The results showed that lead affected the growth of E. agglomerans. The OD value has a negative relationship with the concentration level of Pb. The ODs were decreased from 2.867 to 1.242, using Pb level from 0 ppm to 20 ppm. Therefore, it proved that E. agglomerans could reduce heavy metals concentration in local river in Central Java Province. This research was the first report on E. agglomerans activity on heavy metal in contaminated water. This result can be used as a reference for industrial sites near the river to treat their wastewater before discharging it to the river body to preserve its water purity.
Biosorption of Heavy Metal Pollution by Enterobacter agglomerans Nur Kusuma Dewi; Ibnul Mubarok; Ari Yuniastuti
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20471

Abstract

Biosorption is a new waste treatment technology that can eliminate toxic heavy metals. Biosorption can be considered as an environmentally friendly alternative technology to treat industrial liquid waste that is economically proper to use. One of them is biosorption that utilizes the microorganisms’ absorption ability, especially bacteria that can absorb heavy metals in waters, such as Enterobacter agglomerans. This research aimed to determine the ability of E. agglomerans in reducing heavy metals pollution in local river. The research employed the measurement of the effect of lead (Pb) to E. agglomerans growth using Optical Density (OD) at wavelength 600 nm. The colony numbers were calculated using a standard curve. While the ability of E. agglomerans to reduce heavy metals concentration in liquid media was measured using AAS with a wavelength of 240 nm. The results showed that lead affected the growth of E. agglomerans. The OD value has a negative relationship with the concentration level of Pb. The ODs were decreased from 2.867 to 1.242, using Pb level from 0 ppm to 20 ppm. Therefore, it proved that E. agglomerans could reduce heavy metals concentration in local river in Central Java Province. This research was the first report on E. agglomerans activity on heavy metal in contaminated water. This result can be used as a reference for industrial sites near the river to treat their wastewater before discharging it to the river body to preserve its water purity.
EFEK PERENDAMAN INFUSA DAUN SALAM (Syzygium polyanthum) TERHADAP KUALITAS DAGING AYAM POSTMORTEM Feri Dwi Agustina; Priyantini Widiyaningrum; Ari Yuniastuti
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2271

Abstract

Tujuan penelitian ini untuk mengevaluasi efek perendaman daging ayam broiler dalam konsentrasi infusa daun salam yang berbeda. Penelitian menggunakan desain faktorial dengan 3 taraf konsentrasi infusa (0%; 5%; dan 10%) serta dua waktu pengamatan yaitu 0 dan 8 jam setelah perlakuan perendaman. Parameter yang diukur meliputi rata-rata bilangan peroksida, pH, kadar air dan water holding capacity (WHC). Data dianalisis menggunakan analisis varian dan uji perbedaan akibat perlakuan dilakukan dengan uji beda nyata terkecil. Hasil penelitian menunjukkan bahwa pengukuran pH dan WHC sesaat setelah perlakuan memperlihatkan perbedaan nyata (P0,05), sementara bilangan peroksida dan kadar air tidak berbeda nyata. Pada 8 jam setelah perendaman, terlihat bahwa nilai peroksida, pH, kadar air dan WHC menunjukkan perbedaan nyata. Penelitian ini menyimpulkan bahwa infusa daun salam mampu memperlambat laju bilangan peroksida, memperlambat penurunan pH dan memperlambat penurunan WHC.The aim of this study was to evaluate the effects of the immersion of chicken meat in the bayleaf extract and the time of measurement on the quality of the meat. This was a factorial experiment with three levels of bayleaf extract concentrations (0%, 5% and 10%), and two time of measurement (at 0 and 8 hours after the treatment). The parameters observed were the average peroxide value, the pH, the water content and the water holding capacity. The data were analyzed using ANOVA and followed by the least significant difference test for any significant difference. The results showed that the pH and the water holding capacity were significantly different (P0.05), whereas the peroxide value and the water content were not significantly different. Nevertheless, at eight hours after the immersion it was seen that the peroxide value, the pH, the water content and the water holding capacity were significantly different. It was concluded that the extract of bayleaves might slower the rate of the peroxide value, could decrease the pH, and decrease the water holding capacity.
PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl R Susanti; Fidia Fibriana; Ari Yuniastuti
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12177

Abstract

Phylogenetic or population genetic studies have been focused on finding genetic variations in mitochondrial DNA (mtDNA) because mutations in this region contained five times higher than other genetic materials. The aim of this research was to generate PCR-RFLP D-loop mtDNA profiles of some Indonesian domestic waterfowl. The samples were 35 local ducks, 5 muscovy duck, and 5 geese. These local ducks included on seven types of Javanese ducks in Central Java, i.e. Magelang duck, Peking duck, Pengging duck, Tegal Branjangan duck, Tegal Jarakan duck, Tegal Blorong duck, and Tegal Lemahan duck. PCR technique was used to amplify the D-loop genes, and RFLP analysis of the D-loop was performed with AluIand RsaI. The results show that muscovy duck has no restriction sites in D-loop gene. Moreover, goose has a restriction site of AluI in D-loop gene at 550 bp and 187 bp; and it has no restriction site of RsaI. The analysis of RsaI-RFLP in mtDNA D-loop region of ducks resulted in 1 restriction fragment pattern which can be applied in the identification of duck to differentiate it from goose and muscovy duck. PCR-RFLP analysis duck D-loop mtDNA using AluI and RsaI restriction enzyme resulted in 3 combinations of restriction fragment pattern shown in 3 haplotypes (A, B, and C). Genetic diversity of Indonesian domestic waterfowl population could be identified by using PCR-RFLP analysis on mtDNA D-loop region. So far, there are few results of research on Indonesian domestic waterfowl genetic characteristics based on PCR-RFLP mtDNA D-loop marker. Also, this research has a good contribution on genetic characterization of local duck. The characterization is an important aspect to maintain breed quality and manage the genetic resources of Indonesian germplasm and in facilitating the breeding program. 
Efektivitas Seng (Zn) sebagai Imunostimulan dalam Produksi Reactive Oxygen Intermediate pada Mencit Balb/C yang Diinfeksi Salmonella typhimurium Ari Yuniastuti; Nugrahaningsih W H; Zunikhah Zunikhah
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1151

Abstract

This study aims to prove the effectiveness of zinc as a micronutrient to increaseimmunostimulatory Reactive Oxygen Intermediate (ROI) production in balb/c miceinfected by Salmonella typhimurium. Research was designed using randomized thePost Test Only Control Group Design. The sample of 24 male mice balb/c are groupedinto four groups, i.e. one control group and three treatment groups (treatment dose of 78ppm/day; 169 ppm/day and 260 ppm/day) intraperitoneally for 14 days with each groupconsisting of six rats. The independent variable is the provision of zinc with various doses,dependent variable is the ROI production of macrophages, which are checked by usingNitroblue Tetrazolium (NBT) Reduction Assay, and control variables are body weight,gender and age. All mice infected with Salmonella typhimurium 105 intraperitoneally onday 8th. At the end of treatment mice were killed and was examined for levels of ROI.Inspection results were analyzed by Kruskal Wallis test. Result showed that doses ofzinc of 78 ppm/day have immunostimulatory effective as the highest ROI productionof mice Balb/c infected with Salmonella typhimurium.Keywords: immunostimulant, Reactive Oxygen Intermediate, zinc (Zn)
STATUS ANTIOKSIDAN GLUTATION PADA PASIEN TUBERKULOSIS PARU DI BALAI KESEHATAN PARU (BKPM) MAKASSAR Ari Yuniastuti; Irawan Yusuf; Muh Nasrum Massi; Budu -
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2745

Abstract

AbstrakAntioksidan merupakan senyawa untuk pertahanan tubuh host terhadap radikal bebas. Pada pasien tuberkulosis paru (TB paru), rendahnya antioksidan menyebabkan kerusakan jaringan. Salah satu antioksidan tubuh adalah glutation. Rendahnya glutation berhubungan dengan gangguan sistem imun, sehingga menyebabkan keparahan penyakit pada pasien TB paru. Penelitian ini bertujuan mengetahui kadar glutation plasma penderita tuberkulosis paru. Desain penelitian ini adalah kohort prospektif. Sampel TB paru dipilih di Balai Besar Kesehatan Paru Masyarakat (BBKPM), Makassar yang sesuai dengan kriteria inklusi dan eksklusi dan sesuai kadatangan (consecutive sampling). Dua mililiter darah diambil dari setiap sampel, kemudian dilakukan pemeriksaan kadar glutation dengan ELISA. Hasil penelitian menunjukkan bahwa kadar glutation sampel pasien TB paru sebelum pemberian obat antituberkulosis (OAT) sebesar 0,48383±0,0344 mM dan setelah pemberian OAT sebesar 0,49465±0,0371 mM. Setelah pemberian OAT kadar glutation mengalami peningkatan sebesar 2,2% meskipun masih lebih rendah dari kadar glutation normal. AbstractAntioxidants prevent hosts against free radicals. In pulmonary tuberculosis (TB) patients, the low level of antioxidants may cause tissue damage in oxidative hosts. One of antioxidants is glutathione. The poor glutathione level correlates with immune system disorder. The current study aimed to determine glutathione plasma levels in pulmonary tuberculosis patients. The study design was prospective cohort. Pulmonary TB samples were obtained from patients in the Lung Health Centre (BBKPM) in Makassar, and they were selected based on the inclusive and exclusive criteria and on their admittance times (a consecutive sampling technique). Two mililiters of blood was taken from each sample and the glutathione level was examined using ELISA. The study indicated that the glutathione level of the pulmonary TB patients before administered with antitubeculosis drug was 0.48383±0.0344 mM and after antituberculosis drug was 0.49465±0.0371 mM. After administration of antituberculosis drug the glutathione level increased by 2.2%, although still lower than normal glutathione level.
Co-Authors Abdurrahman Ali, Nur Aini Abudu, Komaria Ode AD Safarina Adevia Maulidya Chikmah, Adevia Maulidya Adhisti Lutfia Hanan Adhisti Lutfia Hanan Adiba, Daniar Arof Adiba, Daniar Arof Aennie Widiastuti, Vinka Afniyati, Nur Afniyati, Nur Agnes Dwi Safarina Agnes Dwi Safarina Aidah, Nur Aisa Nikmah Rahmatih Amalia, Devi Andreas Priyono Budi Prasetyo Arif Kurniadi Ariyanto, Andhika Arum Kartikadewi Arum Kartikadewi Arum Kartikadewi Asih Kuswardinah Asyumdah, Asyumdah Budi Laksono Budi, Dini Wurnaning Budu - Candrawan, Roni Prasetyo Darmayanti, Mey Linda Devi Dwi Jayanti Dewi Mustikaningtyas Dina Nur Anggraini Ningrum Dinda Fuji Islamiyanti Dyah Rini Indriyanti Eko Farida Elissa, Intan Ayu Ell Khaq, Arrina Ely Rudyatmi, Ely Eunike Raffy Rustiana Evi Widowati F. Putut Martin HB Farida, Eko Fathnin, Fildza Huwaina Feri Dwi Agustina Feri Dwi Agustina Fidia Fibriana Fitri Arum Sasi Fitri Arum Sasi, Fitri Arum Halimah, Khori Handayani, Kasmini Hidayah, Arifatul Hidayah, Salis Nur I Wayan Suardana Ibnul Mubarok Ibnul Mubarok Indah Hartati Innayah, Nurul Irawan Yusuf Irawan Yusuf Irwan Irwan Isnaeni, Lili Astuti Isnaeni, Lili Astuti Isti Mulyawati Istianah Istianah Istiqomah Dwi Andari, Istiqomah Dwi Istiqomah, Annisa Aulia Jiwanjaya, Yoga Juwita, Fitra Kamala Nuriyah Kamilatussainah Kamilatussainah Kamisih, Kamisih Karomika, Ati Kasimi, Oktia Woro Khikmatul Maula, Lia Kirana Rannoe Tirta Kirana Rannoe Tirta Krispinus Kedati Pukan lathifah, Ummu Lindasari, Pamela Lisdiana Lisdiana Lucita, Fifty K. Lyndasari, Pamela Mahalul Azam Martien Herna Susanti Mayang Siwi Dewayanti, Mayang Siwi Merzistya, Aufiena Nur Ayu Mohamad Endy Y Yulianto Muchamad Dafip Muchamad Dafip Muh Nasrum Massi Muh. Nasrum Massi Nabila Azzahra Luthfiah Nabila Azzahra Luthfiah Nadhifah, Shiliya Lana Nadhifah, Shiliya Lana Nbaia, Sara M Mohamed Negarani, Eryca Nur Bela Negarani, Eryca Nur Bela Niken Subekti Ning Setiati Ning Setiati Nirmala Al Fatiah Nirmala Al Fatiah Noor Aini Habibah Noor Hidayah Nugrahanigsih WH Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih W H Nugrahaningsih W H Nugrahaningsih WH Nugrahaningsih WH Nugrahaningsih WH, Nugrahaningsih Nur Kusuma Dewi Nur Rahayu Utami NURUL HIDAYAH Oktia Woro Oktia Woro Kasmini H Oktia Woro Kasmini Handayani Pramesti Dewi Pramusinta, Linda Prasetyowati, Tri Ari Priyantini Widiyaningrum R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R. Susanti Rahayu, Rr. Sri Ratna Rahmatih, Aisa Nikmah Rahmatika Saputri R Ratna Rahayu, Sri Ratna Rahayu, Sri Resti Fitriati Retno Sri Iswari Retno Sri Iswari Rofika, Ana Rohmah, Amalia Nor Rr. Sri Ratna Rahayu Sa’adah, Siti Rofiatus Saiful Ridlo Satriani Satriani Sekar Arum, Vriska Roro Setyoko Setyoko Sholikah, Anik Sholikah Shovrotul Khoiriyah, Ely Siti Alimah Siti Alimah Siti Baitul Mukarromah Siti Isnaeni SITI MAHMUDAH Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ratna Rahayu, Rr Sugianto Sulasfiana Alfi Raida, Sulasfiana Alfi Sulistiani, Duwi Sulistyawati, Anita Syarifah, Aidah Nur Talitha Widiatningrum Totok Sumaryanto Florentinus, Totok Sumaryanto Tyas Agung Pribadi Umam, Syaiful Ishlahul Utami, Hanna Dewi Utami, Hanna Dewi Vilda Ana Veria Setyawati Vilda Ana Veria Setyawati Virtanti, Uji Wahyuningtyas, Arini Tri Widya Hary Cahyati Wiwi Isnaeni Woro K H, Oktia Yanti, Erny Yuni Yanuarita Tursinawati Yanuarita Tursinawati Yanuarita Tursinawati Yanuarita Tursinawati Yuni Wijayanti yuniarti, christina ary Yusdike Adystia Zunikhah Zunikhah Zunikhah Zunikhah