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KARAKTER MORFOMETRIK DAN MORFOLOGI ABALON Haliotis squamata Reeve 1846 DI PESISIR SELATAN JAWA DAN BALI Syamsul Bachry; Dedy Duryadi Solihin; Rudhy Gustiano; Kadarwan Soewardi; Nurlisa A. Butet
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 11 No. 2 (2019): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (662.378 KB) | DOI: 10.29244/jitkt.v11i2.24672

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The standard of measurement of abalone is very important because it can help to identify accurately abalone shellfish based on shell morphology. This research was aimed to examine the truss morphometric and morphologycal characters of Haliotis squamata intraspecies in the southern coastal Java and Bali. The research was conducted from December 2014 to August 2016. Abalone was collected based on the purposive sampling method, it was then identified. The shellfish of abalone was measured and analyzed by using principal component analysis (PCA), canonical discriminant analysis and cluster analysis. The results showed that PCA was able to separate Haliotis squamata populations from Java and Bali using combination of PCIII and PCIV based on the factor coefficient values. The key characters that separated Haliotis squamata from the population of Java and Bali were the combination of characters BF (0.535) for PCIII and characters CH (0.522) for PCIV. Canonical discriminant analysis showed that Bali was the highest sharing component value (100%) of intra population and also the lowest sharing component of inter population (0%). The highest percentage of similarity was 99.91% that indicated the population of Binuangeun and Pangandaran, while the lowest was 99.31% for the population of Banyuwangi and Bali. The morphological characteristics of Haliotis squamata species in several locations showed specific characters such as color patterns, textures and shapes.
Kajian Penanda Genetik Gen Cytochrome B Pada Tarsius sp. =Study of Genetic Marker on Cytochrome B Gene of Tarsius sp. Rini Widayanti; Dedy Duryadi Solihin; Dondin Sajuthi; RR. Dyah Perwitasari
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1938.625 KB) | DOI: 10.22146/jsv.349

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Tarsius merupakan salah satu satwa endemik Indonesia yang keberadaannya mulai memprihatinkan. Konservasi sebagai salah satu cara untuk pelestarian satwa ini akan lebih terarah dan berhasil guna apabila karakteristik dan keragaman sumber genetiknya diketahui dengan pasti. Tujuan dari penelitian ini adalah mengkaji penanda genetik spesifik gen cyt b pada Tarsius sp. Pengurutan hasil PCR menggunakan primer H 15149 pada gen cyt b didapatkan urutan basa sebesar 276 pb (menyandi 92 asam amino. Fragmen cyt b hash! pengurutan disejajarkan berganda dengan primata lain dari data Genbank dengan bantuan perangkat lunak Genetyx-Win versi 3.0 dan Clustal W, kemudian dianalisis dengan menggunakan program MEGA versi 3.1. Dari hasil analisis diperoleh 14 situs asam amino yang berbeda. Tarsius dianae memiliki 12 situs asam amino (asam amino ke 2, 6, 9, 22, 23, 29, 39, 41, 42, 45, 55 dan 85), T. spectrum memiliki 7 situs asam amino (asam amino ke 2, 6, 9, 41, 45, 55 dan 85) dan T bancanus memiliki 2 situs asam amino ( ke 23 dan 45) yang dapat digunakan sebagai penanda genetik. Lima asam amino unik ditemukan pada T dianae, yaitu pada situs asam amino ke 6 (valina), ke 22 (alanina), ke 29 (alanina), ke 39 (serina) dan ke 42 (valina). Jarak genetik berdasar nukleotida cyt b yang dihitung menggunakan model 2 parameter Kimura ditemukan nilai paling kecil sebesar 0,7%, nilai paling besar 22,3% dan rata-rata 13,1%. Filogram menggunakan metode neighbor joining berdasar hasil urutan nukleotida dan asam amino cyt b tersebut dapat dijadikan pembeda masing-masing spesies Tarsius.
Keragaman Morfometrik dan Gen cytochrome b DNA Mitokondria Ompok hypopthalmus di DAS Batang Hari. Abdul Rahman Singkam; Dedy Duryadi Solihin; Ridwan Affandi
Biospecies Vol. 11 No. 2 (2018): Volume 11, No.2, July, 2018
Publisher : Universitas Jambi

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This research aims to analyse the variation on morphometric and cytochrome b (cyt b) DNA mitocondria gene of Ompok hypopthalmusin Batang Hari river. Cyt b DNA mitocondria gene was amplified by polymerase chain reactionand targeted1104bp nucleotids. The results show there is no significant differentiation on morphometric structure of the samples. This result is consistent for both of morphometric (p=0.96) and index (p=0.99). Amplification of cyt b DNA mitocondria gene generates927 bp nucleotids with cytosin as the majour component and guanin as the lowest. Only one nucleotide(0.11%) is vary among all the sequences i.e.883thof the partial cyt b gene. The 883th nucleotidein O. hypopthalmus Simpang and Sungai Bengkal is guanin, while inMandiangin dan Pelayangan is adenin. This subtitution occured on the first codon and changed the 295th putative amino acid ofcyt b gene. The putative amino acid in O. hypopthalmus Simpang-Sungai Bengkal is alanina (GCC), while inMandiangin-Pelayangan is treonina (ACC).
Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang I Gusti Agung Arta Putra; Dondin Sajuthi; Dedy Duryadi Solihin; Raden Roro Dyah Perwitasari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .
Tingkah Laku Menetas Piyik Burung Weris (Gallirallus philipensis) dan Burung Dewasa dalam Penangkaran (HATCHING BEHAVIOR AND BEHAVIOR IN CAPTIVITY OF GALLIRALLUS PHILIPPENSIS) Lucia Johana Lambey; Ronny Rachman Noor; Wasmen Manalu; Dedy Duryadi
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The study of behavior has a very important role in wildlife management process both for cultivationand for preservation in nature. The purpose of this study was to observe and document behaviors and dailyactivities of weris. Observation of hatching process and behavior of juvenile weris used direct observationmethod, while the behavior of adult weris in captivity used scanning sampling method. The data wereanalyzed descriptively. Weris had six stages of hatching processes starting from the breakdown of the eggshell until the chick moved out from the shell. The placement of the eggs in hatching machine was byputting the dull part on upside. Weris is a diurnal bird that active in the morning until late afternoon.Young and adult weris birds had different activities, i.e., adult birds did bathing and flying activities,while the young birds just rest and sleep. Observation on behavior of adult weris (n = 10) showed that thehighest activity for 12 hours was moving (314,6 minutes), then followed by resting (283,1 minutes), eating(51,8 minutes), drinking (29 minutes), bathing (22,9 minutes), and the lowest was preening (18,6 minutes).Inherited behavior pattern, such as bathing, did not change, eventhough the environment changed.
Variasi Kolesterol Plasma Individual Monyet Ekor Panjang (Macaca fascicularis) sebagai Respons terhadap Diet Aterogenik IPB-1 [INDIVIDUAL PLASMA CHOLESTEROL VARIATION OF CYNOMOLGUS MACAQUE (MACACA FASCICULARIS) IN RESPONSE TO IPB-1 ATHEROGENIC DIET] Achmad Taher; Dedy Duryadi Solihin; Sulistiyani Sulistiyani; Dondin Sajuthi; Dewi Apri Astuti
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The effectiveness of atherogenic diet in raising plasma cholesterol level of cynomolgus macaques(Macaca fascicularis) can be predicted for population, but not for individuals.This study aimed to evaluatethe individual plasma cholesterol of cynomolgus macaques in response to IPB-1 atherogenic diet. Theanimals under study were 22 adult malemonkeys from the animal facility of Primate Research CenterBogor Agricultural University (PSSP IPB). All animals were intervened with the IPB-1 atherogenic diet forthree months and individual plasma cholesterol wasevaluated in a monthly basis.The results showedthat the monkeys’ plasma cholesterol had increased significantly (P <0.05) after one month of interventionperiod and the increases were very significantly different (P <0.01) after three months. Individually, increasesin plasma cholesterol varied among animals. Based on these variations, 2 animals were categorised ashypo-response, 18 animals as hyper-response and 2 animals as extreme.This study showed the variationof individual plasma cholesterol of cynomolgus macaques in response tothe IPB-1 atherogenic diet so thatthe animals might be selected based on the their responsiveness.
Keragaman Fenotipik dan Pendugaan Jarak Genetik pada Ayam Lokal dan Ayam Broiler Menggunakan Analisis Morfologi (PHENOTYPIC VARIATION AND ESTIMATION GENETIC DISTANCE BETWEEN LOCAL CHICKEN AND BROILER CHICKEN USING MORPHOLOGICAL ANALYSIS) Harini Nurcahya Mariandayani; Dedy Duryadi Solihin; Sri Sulandari; Cece Sumantri
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This aim of the research was to study the morphological characteristic and estimating genetic distancebetween local chicken and broiler chicken with discriminant and canonical analysis. This research washeld in Faculty of Animal Husbandry, Bogor Agricultural University, using 25 sentul chickens,  25  kampongchickens, 25 kedu chickens , 25  pelung chickens and 25 broiler chickens. The variable as the length ofshank, beak length, back length, chest depth and chest width were measured in this study. The collecteddata were analyzed by using SAS  and SPSS package program. Kampung chickens were mixed with sentulchickens (17.60 %) and kedu chickens (17,70 %). Kedu, kampong,  and  sentul chickens have a relativelyclose genetic distance   compared the genetic distance to pelung chickens with the kampung, sentul, andkedu chickens. Fenogram tree show that there were three separate groups of chickens at the age of eightweeks i.e. : (1) pelung chickens (2), kedu, kampong, and sentul chickens, (3) broiler chickens.  Fenogram treealso shows two separate groups : (1) pelung chickens (2) kedu, kampong, and Sentul chickens (at the age of28 weeks chicken).  The crossbreed between kedu and sentul chickens, also have a relatively close geneticdistance. The phenotypic size of  chickens giving a strong influence on the distinction variable of chickengroups were body length and chest circumference.
Karakteristik Morfologi, Perbedaan Jenis Kelamin, dan Pendugaan Umur Burung Weris (Gallirallus philippensis) di Minahasa, Sulawesi Utara (MORPHOLOGICAL CHARACTERISTICS, SEX DIFFERENCES, AND AGE ESTIMATION OF WERIS (GALLIRALLUS PHILIPPENSIS) FROM MINAHASA Lucia Johana Lambey; Ronny Rachman Noor; Wasmen Manalu; Dedy Duryadi
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study aim  to observeb Gallirallus philippensis  similarities between different sites in Minahasa,to detect sexual dimorphism, and to estimate the bird age based on morphological characteristics.  A totalof 35 birds  were collected  from four locations i.e. Tondano, Papontolan, Ranoyapo, and Wusa in MinahasaNorth Sulawesi.   The result showed that there were no differences in the length  of beak, wing, and shankbetween  birds from  the different locations.  Moreover, based on the Principle Component Analysis (PCA)the bird’s shank and tail’s length were the main component.  Based on cluster analysis, the birds from fourlocations had a close similarities.  The male bird’s body weight, beak length, and beak width were largercomponent to the female’s.  The bird’s age could be best predicted by their iris colour,  beak colour, feather colourof the neck and the growth of the wing feathers, respectively.
Acanthophyllia deshayesiana (Michelin, 1850) Coral Species Is Not Synonym With Cynarina lacrymalis (Milne Edwards & Haime, 1848) Robba Fahrisy Darus; Neviaty Putri Zamani; Suharsono Suharsono; Dedy Duryadi Solihin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 3 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.922 KB) | DOI: 10.14710/ik.ijms.21.3.107-116

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Acanthophyllia deshayesiana has a different habitat with Cynarina lacrymalis in the nature, but they have same character on living forms, diameter, and height of corallite. Both of these species are considered synonym, thus it needs verification study to describe whether it is synonym species or not based on morphological data. Eleven descriptive characters and seven morphometric characters were used to verify the synonym species of these coral. Descriptive data were performed by scoring method, while morphometric data were obtained from morphometric. Morphometric data were analyzed by Correspondence Analysis of Principal Coordinates (CAP) and Agglomerative Hierarchical Cluster (AHC), while descriptive data were analyzed by UPGMA (Unweight Pair Group Method with Arithmetic Mean). The result showed that both of these coral cannot differentiate based on morphometric measurement. It can differentiate significantly based on descriptive characters, so both of these coral are not synonym. Keywords: synonym, morphometric, descriptive, Cynarina lacrymalis, Acanthophyllia deshayesiana
Genetic Profile Assessment of Giant Clam Genus Tridacna as a Basis for Resource Management at Wakatobi National Park Waters Muhammad Nur Findra; Isdradjad Setyobudiandi; Nurlisa Alias Butet; Dedy Duryadi Solihin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.089 KB) | DOI: 10.14710/ik.ijms.22.2.67-74

Abstract

Giant clam population has been decreased in a few years. Resource management requires information from various aspects, such as ecological, population, and other aspects. This study was aimed at assessing the genetic profile of Tridacna giant clam in Wakatobi National Park waters using Cytochrome oxidase subunit I (COI) genetic marker. Sample collection was conducted around the three main islands, i.e., Wangi-wangi, Kaledupa, and Tomia. Genetic analysis using COI gene may contribute in identifying giant clams up to the species level and showed the relationship among species. The research found 41 specific nucleotide sites for the clams. T. crocea, T. squamosa and T. maxima had 2, 15 and 24 sites, respectively. COI gene as a biological marker was able to separate groups of giant clam by species. Nucleotide variation of T. crocea from Wakatobi was the highest among other locations, so it could be used as a genetic source for translocation and domestication. Keywords: cytochrome oxidase subunit I, specific nucleotide, Tridacna, Wakatobi National Park
Co-Authors Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Mardiastuti Ani Suryani Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Any Aryani Ardi Kapahang Arief Boediono Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Dedi . Soedharma Dedi Soedharma DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia DIAH ISKANDRIATI Diah Iskandriati DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa EVY AYU ARIDA Evy Ayu Arida Fahma Wijayanti FAHRI FAHRUDIN Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra Hadi S Alikodra HADI SUKADI ALIKODRA HAJRIAL ASWIDINNOOR Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Joko Pamungkas Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo Lilik Budi Prasetyo Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Butet, Nurlisa Prasetyo Prasetyo Priyono, Siti N. Retno Damayanti Soejoedono RICHARD F GRANT Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi RIDWAN AFFANDI Rini Widayanti Robba Fahrisy Darus ROEDHY POERWANTO Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito Suharsono Suharsono SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Surjono Surjokusumo Syaiful Anwar Syamsul Bachry Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yusnarti Yus