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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences

SRI HENDRASTUTI HIDAYAT

Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680

Author-ID : 385601

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Veterinary

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Penularan Potyvirus Penyebab Penyakit Mosaik pada Tanaman Nilam melalui Vektor Aphis gossypii Rita Noveriza; Gede Suastika; Sri Hendrastuti Hidayat; Utomo Kartosuwondo
Jurnal Fitopatologi Indonesia Vol. 8 No. 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Patchouli (Pogostemon cablin) is an economically important plant due to its capability to produce essential oil, i.e. patchouli oil. Pest and diseases may contribute to low production of patchouli oil in Indonesia. Aphids are commonly found associated with patchouli plants showing mosaic symptoms, indicated possible relationship between aphids and mosaic virus. Early diagnosis showed that mosaic disease on patchouli is caused by Potyvirus infection. A study was conducted to identify those aphid species and to examine relationship between the aphid species and mosaic virus. Two aphid species commonly found in patchouli plants in Indonesia are Aphis gossypii and Brachycaudus sp. Further study on the ability of A. gossypii to transmit Potyvirus revealed the possible non-persistent relationship, with 25 min optimum pre-acquisition starvation, 15 min acquisition period and 4 hours optimum inoculation feeding period, respectively.Key words: Aphis gossypii, Brachycaudus, nilam, Pogostemon cablin, Potyvirus

Ketahanan Biokimia Tanaman Cabai terhadap Begomovirus Penyebab Penyakit Daun Keriting Kuning Rokhana Faizah; Sriani Sujiprihati; Muhamad Syukur; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant biochemical resistance towards virus infection can be evaluated through measurement of salicylic acid accumulation and peroxidase concentration. A research was conducted to study biochemical resistance of chillipepper to infection of Pepper yellow leaf curl Begomovirus (PYLCV) the causal agent of pepper yellow leaf curl disease. Virus inoculation was done using insect vector, Bemisia tabaci. Salicylic acid accumulation and peroxidase concentration on 6 chillipepper genotypes (35C2, IPB C15, IPB C26, IPB C10, IPB C14, dan IPB C12) was measured 120 h after inoculation. The results showed that salicylic acid accumulation and peroxidase concentration were higher on plants after virus infection. Susceptible genotypes (35C2, IPB C15, IPB C26) tend to have higher salicylic acid accumulation and peroxidase concentration than those of resistant genotype (IPB C12). Positive correlation was observed between biochemical resistance (salicylic acid accumulation) and structural resistance (length of palisade meshophyll cell).Key words: Begomovirus, Pepper yellow leaf curl virus, peroxide enzyme, resistance mechanism, salicylic acid

Infeksi Papaya ringspot virus pada Tanaman Pepaya di Provinsi Nanggroe Aceh Darussalam Sri Hendrastuti Hidayat; Sari Nurulita; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Severe mosaic symptoms was observed on papaya plants in Meusa village, Kutablang sub district, Bireun district and Lambaro Teunom village, Lembah Seulawah sub district, Aceh Besar district. Systemic mosaic was found in leaves, stem, twig, and fruit. Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to detect the virus causing mosaic symptoms using universal primer for Potyvirus. Amplification of 320 bp DNA fragment was successfully obtained from leaves and fruits bearing severe mosaic symptoms. Nucleotide sequencing was proceeded using the DNA amplicon as the template. Further sequence analysis indicated that Potyvirus isolates from papaya in Aceh show the highest homology (92.7% to 94.7%) with Papaya ringspot virus (PRSV) from Philippines, Thailand, and Indonesia. This is the first report on PRSV infection on papaya in Nanggroe Aceh Darussalam province. Key words: Potyvirus, RT-PCR, sequencing

Tomato yellow leaf curl Kanchanaburi virus Penyebab Penyakit Mosaik Kuning pada Tanaman Terung di Jawa Tega Kintasari; Dwi Wiyati Nurul Septariani; Sri Sulandari; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow and yellow mosaic symptoms was observed on eggplant (Solanum melongena) around Bogor, Bandung, Pati, Rembang, and Bantul during a survey in early 2013. Polymerase chain reaction based detection was carried out using total DNA isolated from symptomatic leaf samples and a pair of Begomovirus-universal primers, SPG1/SPG2. The expected size (~900 bp) amplicon was detected from all five symptomatic samples, indicating the presence of Begomovirus infection. Each amplicon was sequenced followed by basic local alignment search tool analysis. It was revealed that the sequence had the highest homology (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) sequence. This is the first report on TYLCKaV infection on eggplant in Indonesia.

Cendawan Endofit Nonpatogen Asal Tanaman Cabai dan Potensinya sebagai Agens Pemacu Pertumbuhan Evan P. Ramdan; Widodo Widodo; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 5 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic fungi have been reported to have mutual symbiosis with their host. This research aimed to obtain nonpathogenic isolates of endophytic fungi from roots, stems, and branches of chili pepper. Endophytic fungi were isolated on 10% malt extract agar (MEA) after serial surface sterilization of plant parts. The effect of endophytic fungi on the development of chili pepper seeds were tested by growing seeds on media containing endophytic fungi isolate. As many as 138 isolates of endophytic fungi were successfully isolated from chili pepper plants from Garut and Bogor. Based on colony morphology the isolates can be differentiated into 9 morphotypes. Among the total isolates, 13.04% are nonpathogenic. Some of them (7,25%) do not effect seedling significantly compared to control and some isolates (5.79%) caused better seedling growth than control. Endophytic fungi having the potential to promote plant growth was identified as Aspergillus sp., Fusarium sp., Penicillium sp., sterile hyphae 1, 2, and 3.

Penggunaan Galur Lemah Chili veinal mottle virus untuk Proteksi Silang Asniwita Asniwita; Sri Hendrastuti Hidayat; Gede Suastika; Slamet Susanto; Sriani Sujiprihati
Jurnal Fitopatologi Indonesia Vol 9 No 5 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Inoculation of mild virus strain prior to severe virus strain to protect plant against viral disease is the principle of cross protection. Five mild strains of Chili veinal mottle virus (ChiVMV), i.e. -KAR, -SPR, -SKT, -CSR, and -PGL were used as cross protection agent to protect chili pepper plants against severe strain infection of ChiVMV-CKB. The mild strains were inoculated mechanically prior inoculation of severe strain and the efficiency of cross protection was evaluated by observing symptom development and measuring crop yield. Inoculation of mild strains 7 days prior inoculation of severe stain was not able to protect the plant from infection of severe strain ChiVMV-CKB. Protective effect was observed when mild strains were inoculated at 14, 21, and 28 days prior inoculation of severe strain. Symptom development was suppressed or delayed, and crop yield was not significantly different with healthy plants. It was suggested that to obtain the best protection against severe strain, the mild strain should be applied as early as possible before the occurrence of severe strain infection.

Sensitivitas Metode Serologi dan Polymerase Chain Reaction untuk Mendeteksi Bean Common Mosaic Potyvirus pada Kacang Panjang Sherli Anggraini; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Mosaic disease in yard long bean is caused by Bean common mosaic potyvirus (BCMV) and has been reported to affect yield. Common method to detect infection of BCMV involves serological assay and polymerase chain reaction (PCR). The aims of this research is to assess the sensitivity of three methods, i.e. Indirect Enzym-Linked Immunosorbent Assay (I-ELISA), Dot Immunobinding Assay (DIBA), and reverse transcription (RT)-PCR as detection method for BCMV infection in yard long bean. Sensitivity level of the methods was evaluated by diluting plant extract and antisera for I-ELISA and DIBA, and cDNA as template in RT-PCR. Virus isolate from Cirebon was maintained in yard long bean in screenhouse and used for the assessment. Absorbance value of ELISA showed that dilution end point for I-ELISA was reached at 10-3 and 10-2 of plant extract and antisera dilution, respectively. Positive infection was still detected using DIBA when the plant extract was diluted up to 10-5 based on development of color intensity on nitrocellulose membrane. Specific viral DNA fragment was still amplified when cDNA was diluted up to 10-4, indicated higher sensitivity level of RT-PCR method.

Respons Lima Varietas Kacang Panjang terhadap Bean common mosaic virus Harwan Susetio; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 4 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Incidence of yellow mosaic disease on yard long bean occurred widely in some areas in West and Central Java in 2008–2009. The causal agent of this disease is Bean common mosaic virus (BCMV) which can be transmitted through seed and aphids. Research was conducted to determine response of five varieties of yard long bean (Vigna sinensis), i.e. Parade, New Jaliteng, Long Silk, Super Sainan, and Pilar to BCMV infection. Yard long bean plants was inoculated by BCMV mechanically, followed by observation of disease incidence and severity, incubation period, flowering time, and yield. All five varieties of yard long bean showed susceptible response to BCMV infection. Incubation period was 6–16 days, disease incidence was 90–100%, and disease severity was 49.1–69.7%. BCMV infection caused severe symptoms of leaf malformation and plant stunting. Flowering time was delayed 2–5 days on infected plants and yield was decreased up to 46.6%. Breeding program to develop resistance varieties to BCMV is necessary.

Keparahan Penyakit Daun Keriting Kuning dan Pertumbuhan Populasi Kutukebul pada Beberapa Genotipe Cabai Nissa Fawwaz Adilah; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 6 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Incidence of yellow leaf curl disease on chili pepper is very common in Indonesia and becomes major production constrain. The causal agent, Pepper yellow leaf curl virus (PYLCV), is only transmitted by insect vector, i.e. whitefly (Bemisia tabaci). Research was conducted to determine response of 6 chili pepper genotypes [Meteor, Rimbun, Tornado, F1(12X14), IPBC12, and 35C2] against infection of PYLCV and population growth of whitefly on each genotypes. Transmission of PYLCV to chili pepper genotypes was done using whitefly. Whitefly was maintained on each chili pepper genotypes to evaluate its population growth. Genotype IPBC12 was resistant to PYLCV having disease severity 0–9.2% and showed mild symptom. Genotypes Rimbun, Meteor, Tornado, F1(12X14), and 35C2 were susceptible having disease severity >20% and showed severe symptom. Resistance of IPBC12 might be correlated with the growth of whitefly on the genotype. The amount of whitefly eggs and its successful rate to become adult was relatively lower in IPBC12 than those in other genotypes. Genotype IPBC12 might be considered as resistance source in developing resistant variety of chili pepper to PYLCV.

Deteksi Virus Utama Bawang Merah dan Bawang Putih dari Daerah Jawa Barat dan Jawa Tengah Kadwati Kadwati; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral disease has been reported to cause significant effect on production of shallot (A. cepa) and garlic (A. sativum). The study was conducted to detect main viruses from leaves and bulbs of shallot and garlic using specific antibodies by ELISA method. Leaf and bulb samples was collected from West Java (Bandung, Bogor and Cirebon), Central Java (Brebes), and Yogyakarta (Bantul). Single as well as mix infection of GCLV, SLV, and Potyvirus was successfully detected using specific antibodies. The average percentage of virus infection in the crop ranged from 11.2–14.3% on shallot, and 14.3% on garlic; whereas in the bulb ranged from 11.2–13.3% on shallot, and 9.18% on garlic.

Co-Authors . SUDARSONO Abdul Muin Adnan ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT AUNU RAUF Ayu Kartini Parawansa Azmi Khoirin Nada Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Erika Rosminim Purba Evan P. Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Gede Suastika GEDE SUASTIKA Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto Giyanto Hamdayanty Hamdayanty Hari Priwiratama Harwan Susetio Heri Harti Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro ISHAK MANTI Ishak Manti Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muhamad Syukur Muhammad Herman Muhammad Taufik MUHAMMAD TAUFIK Naimatul Farida Neni Gunaeni Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purnama Hidayat Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat Rahmi Yunianti Rahmi Yunianti Redy Gaswanto Redy Gaswanto, Redy Reymas M.R. Ruimassa Rina Rachmawati RITA NOVERIZA Rita Noveriza Rita Noveriza Rita Noveriza Rokhana Faizah Roy Ibrahim Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sakinah Inayatur Rizqiyah Sari Nurulita Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Sherli Anggraini Sientje Mandang Sumaraw Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Suryo Wiyono Susanti Mugi Lestari Susanti Mugi Lestari Taufik, Muh Tega Kintasari Titiek Yulianti TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati Ummu S. Rustiani Ummu Salamah Rustiani UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo Utomo Kartosuwondo Vinsen Willi Wardhana Wayan Winasa Widodo Widodo . Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Yudia Nurhaelena Zahratul Millah

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