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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences
SRI HENDRASTUTI HIDAYAT
Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Veterinary

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Journal : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

 1 
IDENTIFIKASI MOLEKULER BEAN COMMON MOSAIC VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK KUNING KACANG PANJANG Melinda .; Tri Asmira Damayanti; Sri Hendrastuti Hidayat
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.316 KB) | DOI: 10.23960/j.hptt.215132-140

Abstract

Molecular identification of bean common mosaic virus associated with yellow mosaic disease on yard long bean. Bean common mosaic virus (BCMV) has been reported as one of the causal agents of yellow mosaic disease on yard long bean in West Java and Central Java. Infected plants showed mosaic, yellowing, and mixture of yellow mosaic. The research was conducted to identify the diversity of BCMV associated with yellow mosaic disease based on coat protein (CP) gene sequences. Symptomatic leaf samples were collected from yard long bean growing areas in several districts in West Java (Bogor, Cirebon, Subang, and Indramayu), and several districts in Central Java (Tegal, Klaten, Solo, Yogjakarta, Sleman, and Magelang). Molecular detection using RT-PCR method was carried out by using specific primer to BCMV which will amplify the CP gene. DNA fragment, + 860 bp in size, was successfully amplified from 8 out of 13 leaf samples, i.e samples from three villages in Bogor District (Cangkurawok, Bubulak, Bojong), and five samples from District of Cirebon, Subang, Solo, Sleman, and Tegal. Sequence analysis of those DNA fragment showed that 4 isolates (Bogor-Cangkurawok, Subang, Solo and Sleman) had the highest homology to BCMV-BlC from Taiwan, whereas 2 isolates (Cirebon and Tegal) had the highest homology to BCMVNL1 from England. Further, phyllogenetic analysis revealed that those of 4 isolates were closely related to BCMV-BlC from Taiwan based on nucleotide as well as amino acid sequences; while those other 2 isolates were closely related to BCMV-NL1 from England based on nucleotide sequences but closely related to BCMV-BlC Y from China based on amino acid sequences. Phyllogenetic analysis showed that those of 6 BCMV isolates separated in two different clusters; 4 isolates (Bogor- Cangkurawok, Subang, Solo, and Sleman) in cluster 1 together with BCMV-BlC from Taiwan, while other 2 isolates (Cirebon and Tegal) in cluster 2 together with BCMV-NL1.
VIRULENSI EMPAT ISOLAT CHILLI VEINAL MOTTLE POTYVIRUS PADA TANAMAN CABAI (CAPSICUM ANNUUM L.) Ifa Manzila; Sri Hendrastuti Hidayat; Ika Mariska; Sriani Sujiprihati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.005 KB) | DOI: 10.23960/j.hptt.211122-129

Abstract

Infection of Chilli veinal mottle potyvirus (ChiVMV) has been reported from chilli pepper growing area in Indonesia. Field observation showed wide variation of symptom development starting from mild mottle to severe mottle and vein banding. A research was conducted to study the difference of isolates of ChiVMV collected from West Java (Cikabayan), Central Java (Karadenan), South Kalimantan (Nusa Indah), and West Sumatera (Tanah Datar) based on their response on 10 genotypes of chilli peppers i.e. Jatilaba, Helem, VC246, Keriting Bogor, PBC485, Titisuper, Beauty Bell, Gelora, IPBC Tanjung, and Keriting Sumatera. Each isolates of ChiVMV was mechanically inoculated to each chilli pepper genotype, and observation was conducted every day to record incubation period, symptoms type, and disease incidence. Cikabayan and Nusa Indah isolates were able to infect all chilli pepper genotypes with the shortest incubation period of 3 days in “Tistsuper” and “Beauty Bell” genotypes; whereas Karadenan and Tanah Datar isolates only infected 4 and 6 chilli pepper genotypes, respectively. Although the 4 isolates of ChiVMV showed differences in their host range, but most of them developed similar symptoms ranging from mottle, vein banding, leaf cupping, and malformation. Based on the host response, ChiVMV isolates from Cikabayan and Nusa Indah were considered more virulent than the isolates from Karadenan and Tanah Datar.
Identifikasi Penyebab Penyakit Daun Keriting Kuning pada Tanaman Mentimun Dwiwiyati Nurul Septariani; Sri Hendrastuti Hidayat; Endang Nurhayati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.026 KB) | DOI: 10.23960/j.hptt.11480-86

Abstract

ABSTRACTIdentification of the causal agent of yellow leaf curl disease on cucumbers. Yellow leaf curl disease has been reported to cause serious diseases and yield losses on tobacco, chilli pepper, and tomato plants in Java. Similar symptoms were observed recently on cucumber plants from several growing areas in West Java (Bogor), Central Java (Tegal and Sukoharjo), and Yogyakarta (Sleman). Symptom variations including mosaic, chlorotic spotting, leaf curling, blistering, vein banding, reduction and distortion of leaf and fruit were observed. Serological detection using Enzyme Linked Immunosorbent Assay (ELISA) showed infection of several viruses. Antibodies specific to Squash mosaic comovirus (SqMV), Zucchini yellow mosaic potyvirus (ZyMV), dan Cucumber mosaic cucumovirus (CMV) were reacted positively with field samples. No serological reactions were observed with antibodies to Tobacco ringspot potyvirus (TRSV) and Watermelon mosaic potyvirus (WMV). Molecular detection approach based on Polymerase Chain Reaction was undergone using universal primers for Geminivirus, pAL1v1978 and pAR1c715. DNA fragment 1600 bp in size, was successfully amplified from leaf samples originated from Tegal, Sleman, Bogor, and Sukoharjo. Further identification by nucleotide sequencing indicated that virus isolates causing yellow leaf curl disease on cucumber have highest homology (95.7% to 98.6%) with Tomato leaf curl New Delhi virus-[Cucumber:Indonesia] (AB613825) from Klaten, Central Java, Indonesia.
Identification of mealybugs on Piper nigrum as vector of Piper yellow mottle virus (Badnavirus: Caulimoviridae) Miftakhurohmah Miftakhurohmah; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; Bonny Poernomo Wahyu Soekarno; Dono Wahyuno
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222144-153

Abstract

Piper yellow mottle virus (PYMoV) is the dominant virus that causes mottle disease in black pepper (Piper nigrum). Two species of mealybugs, Ferrisia virgata and Planococcus minor have been reported as vectors of PYMoV. A different species of mealybug that has never been reported before was found in black pepper. Molecular approaches including total DNA isolation of mealybug collected from the field, mealybugs identification by DNA barcode, detection of PYMoV in single mealybugs, were conducted as an approach to identify the potential of mealybugs as PYMoV vector in the field. Mealybugs were collected from black pepper plants in Cimanggu (Bogor, West Java) and Sukamulya (Sukabumi, West Java). Characters of adult females were observed for morphological identification. Molecular-based identification of the mealybugs and PYMoV involved the following procedures: total DNA isolation, DNA amplification, nucleotide sequencing and sequence analysis. Three species of mealybugs, P. minor, F. virgata and Paracoccus marginatus were confirmed by morphological and molecular identification. This is the first report for the occurrence of P. marginatus in black pepper plants. PYMoV was successfully detected from field samples of F. virgata, P. minor and P. marginatus. This finding indicates the potential of insect vectors for disease spread and distribution.
Co-Authors . SUDARSONO Abdul Muin Adnan ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT AUNU RAUF Ayu Kartini Parawansa Azmi Khoirin Nada Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Erika Rosminim Purba Evan P. Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Gede Suastika GEDE SUASTIKA Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto Giyanto Hamdayanty Hamdayanty Hari Priwiratama Harwan Susetio Heri Harti Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muhamad Syukur Muhammad Herman Muhammad Taufik MUHAMMAD TAUFIK Naimatul Farida Neni Gunaeni Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purnama Hidayat Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat Rahmi Yunianti Rahmi Yunianti Redy Gaswanto Redy Gaswanto, Redy Reymas M.R. Ruimassa Rina Rachmawati RITA NOVERIZA Rita Noveriza Rita Noveriza Rita Noveriza Rokhana Faizah Roy Ibrahim Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sakinah Inayatur Rizqiyah Sari Nurulita Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Sherli Anggraini Sientje Mandang Sumaraw Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Suryo Wiyono Susanti Mugi Lestari Susanti Mugi Lestari Taufik, Muh Tega Kintasari Titiek Yulianti TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati Ummu S. Rustiani Ummu Salamah Rustiani UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo Utomo Kartosuwondo Vinsen Willi Wardhana Wayan Winasa Widodo Widodo . Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Yudia Nurhaelena Zahratul Millah