Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : BIOTROPIA - The Southeast Asian Journal of Tropical Biology

DEVELOPMENT OF MUD CRAB (SCYLLA OLIVACEOUS HERBST) OOCYTE AFTER IN VITRO CULTURE WITH THORACIC GANGLION EXTRACTS OF ESTUARINE CRABS (NEOEPISESARMA LAFONDI JACQUINOT AND LUCAS) Yushinta Fujaya; Dody Dharmawan Trijuno; Sulistiono; Seiichi Watanabe; Arief Boediono
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 14 No. 1 (2007)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.993 KB) | DOI: 10.11598/btb.2007.14.1.22

Abstract

Thorax ganglion was reported as a source of stimulating hormone for gonad development. The aim of this research was to study the development of mud crab (Scylla olivaceous) oocytes cultured in in vitro medium supplemented with thoracic ganglion extracts of Neopisesarma lafondi. Immature crabs with 110 mm carapace width were collected from the mouth of Bawana Marana Rivers, Maros Regency of South Sulawesi, Indonesia. Pieces of ovarian tissues from those crabs were incubated within 24 hours in culture medium (Medium-199 with 100 IU/ml Penicilin-G) supplemented with 2 mg/ml thoracicganglion extract. Result showed that the diameter of mud crab oocytes was increased in congruent with the incubation time. The development was prominent after in vitro incubation for 8 hours (P
PRESERVATION OF GARUT RAMS SPERMATOZOON AS A SOURCE OF MALE GERM PLASM ARIEF BOEDIONO; HERDIS; MUHAMMAD RIZAL'
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 23 (2004)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.463 KB) | DOI: 10.11598/btb.2004.0.23.200

Abstract

This study was conducted to examine the quality of ejaculated sperm by Garut rams to be used for artificial insemination (AI) and viability of sperm that were collected from preserved cauda epididymis  (4°C up to 12 days) for assisted reproductive technology. The semen was collected by artificial vagina, with the sperm motility, live sperm, acrosomal intact, and intact plasma membrane observed. Sperm motility was 75%, while for the live sperm, intact plasma membrane and sperm abnormality were 91.5%, 90.0%, and 1.8%, respectively. In the other study, sperm was collected from cauda epididymis by aspiration method and diluted in different media: 1) Brackett Oliphant (BO) media and 2) modified Phosphate Buffer Saline (mPBS). Evaluation of  sperm motility and intact plasma membrane were conducted after washing, counting and dilution of the sperm. The results of this study showed that the sperm motility and intact plasma membrane could be maintained better in BO rather than PBS medium although they were not statistically different (P>0.05). At day 12 of preservation, the motility and intact plasma membrane of sperm collected from cauda epididymis were 0.7% and 1.33% for motility and plasma membrane intact, respectively. These findings showed that the Garut rams semen was qualified for AI and frozen processing; in vitro embryo production by introducing the assisted reproductive technology such as intracytoplasmic sperm injection (ICSI) could be applied by using the sperm collected from preserved cauda epididymis until 12 days of preservation at 4°C. Keywords :   Reproduction/spcrmatogenesis/inscmination/Garut rams/small ruminant
EMBRYO RECONSTRUCTION BY TRANSPLANTATION OF THE DONOR INNER CELL MASS TO THE RECIPIENT BOVINE BLASTOCYST ARIEF BOEDIONO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 13 No. 2 (2006)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2006.13.2.222

Abstract

In an attempt to produce the interspecies embryo transfer, this study was conducted to evaluate the efficacy of the production of reconstructed blastocyst by transferring the donor ICM into the recipient trophoblast. ICM cells were isolated from the donor blastocyst by immunosurgery method. Zona-free blastocysts were incubated in the medium (TCM-199) containing 20% of the heat-inactivated rabbit anti-bovine-serum. The embryo reconstruction was produced by three different methods. Recipient blastocyst was maintained on the holding pipette by gentle suction, with the ICM in a 9 o'clock position to have the possibility of developing incorporate ICMs (Method I), the ICM was in a 3 o'clock position to break the original ICM during injection (Method 2); cutting the original recipient ICM followed by insertion of the donor ICM (Method 3). Reconstructed blastocysts were then cultured overnight and examined morphologically according to the re-expansion of the reconstructed blastocyst with or without developed donor ICM. According to morphological observation in this study, 37.9% of the reconstructed blastocyst developed with the incorporation of two ICM originally from recipient and donor (Method I), 66.7% of the reconstructed blastocysts developed with a single ICM (Method 2), and 80.0% of the reconstructed blastocyst developed from the ICM originally from donor ICM (Method 3). These results showed that the reconstructed blastocyst is better produced by cutting the original recipient ICM followed by the insertion of the donor ICM (Method 3).Key words: embryo reconstruction, immunosurgery, ICM transfer, bovine.
Co-Authors . Alimuddin . AULANI’AM . Herdis . Yulnawati A.S. Satyaningtijas Achmad Selamet Aku Adi Winarto Adisti Dwijayanti Adkhilni Utami Adkhilni Utami Adrian Situmeang Adrian Situmeang Adrian Situmeang Adrien Jems Akiles Unitly Agus Harsoyo Agus Harsoyo Agus Oman Sudrajat Agus Setiadi Al Azhar Al Mukhlas Fikri AL-AZHAR AL-AZHAR Alfred O. M. Dima Alif Iman Fitrianto Alif Iman Fitrianto Alkaustariyah Lubis Amrozi - Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andri Maruli Tua Lubis Andri Maruli Tua Lubis Andriyanto A Andriyanto Andriyanto AUCKY HINTING Aulia Miftakhur Rahman Bambang Kiranadi Bayu Rosadi Berry Juliandi Bibiana W Lay BIBIANA W LAY Boenjamin Setiawan Boenjamin Setiawan Budiariati, Vista Budiono, Dwi Cece Sumantri Chairun Nisa Citra Noviana Cutnya’ Shaliran Nazlie (Alm) Dedy D. Solihin Diah Nugrahani Pristihadi Dian Anggraini Djaswadi Dasuki Djaswadi Dasuki Djoko Walujo Dody Dharmawan Trijuno Dondin Sajuthi Dwi Haryadi Elpita Tarigan Evy Ayu Arida EVY AYU ARIDA Farid A. Moeloek Ferry Sandra Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Hadi, Restu S. Handina Rakhmawati Handina Rakhmawati Harry Murti Harry Murti Harry Murti Harry Murti Hartanti Kusumaningtyas HERA MAHESHWARI HERDIS Herdis . herdis herdis Heri Sujoko Heru Setijanto I Ketut Mudite Adnyana I Ketut Suatha I Wayan Batan Ichsan Ichsan Iis Diatin Iman Supriatna Indra Bachtiar Indra Kusuma Irma H Suparto Irma Herawati Suparto Irma Suryani Ita Djuwita Ita Djuwita Ita Fauzia Hanoum Ita Fauzia Hanoum, Ita Fauzia Karisma Mardatillah Karisma Mardatillah KARTINI ERIANI Kartini Eriani KARTINI ERIANI Kartiwa, R. Angga Kelvin Yaprianto Kelvin Yaprianto Kelvin Yaprianto Ketut Adnyane Mudite Krido Brahmo Putro Kusdiantoro Mohamad Kusumaningtyas, Hartanti Latifah Kosim Darusman Lea Tarliyah M Agus Setiadi M. Haviz Madihah Madihah Madihah Madihah, Madihah Maman Surachman Mas Rizky A.A. Syamsunarno Min Rahminiwati Miraprahesti, Retti N. Mohamad Fakhrudin Mohammad Ghozali Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGUS SUPRAYUDI Muhammad Gunawan Muhammad Gunawan MUHAMMAD RIZAL Muhammad Rizal MUHAMMAD RIZAL' Muhammad Rosyid Ridlo Muhammad Zairin JR Muhammad Zairin Jr. Mulyoto Pangestu MULYOTO PANGESTU MULYOTO PANGESTU Muslim Muslim Nastiti Kusumorini Nastiti Kusumorini Nazlie (Alm), Cutnya’ Shaliran Nining Handayani Nining Handhayani Noer Muhammad Dliyaul Haq Nurhidayat - Nurhidayat Nurhidayat Nuril Farizah Nursanti, Risa R. Sapto Hendri Boedi Soesatyo Rachmat Herman Rahmaniyah, Wiwit Ridhani Ramadhan Sumarmin Rangga Setiawan Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Resti Rahma Dianti Ridi Arif Rimayanti - Rini Widyastuti Riris L. Puspitasari Riris L. Puspitasari Ronny Rachman Noor Sandy Qlintang SATRIYAS ILYAS Satya Gunawan Seiichi Watanabe Shofwal Widad Shofwal Widad Sigit Prastowo Siti Darodjah Rasad Sony H. Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO SONY HERU SUMARSONO Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih Srihadi Agungpriyono Subangkit, Mawar Sulistiono Sumarsono, Sony H. Sumarsono, Sony Heru Supar - Supar . Sutarya Enus Sutiman Bambang Sumitro TAKDIR SAILI TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Taufik Jamaan Thomas Mata Hine Thomas Mata Hine Trevino A. Pakasi TRINIL SUSILOWATI Tutik Wrediati Tutik Wresdiyati - Tutty Laswardi Yusuf Tuty Laswardi Yusuf Vincentia Maria Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wahyudin Wasmen Manalu Widjiati w Wildan Mubarok Wining Astini Wining Astini Wiwit Ridhani Rahmaniyah Yoga Yuniadi Yuhara Sukra Yulnawati . YULNAWATI YULNAWATI Yundari, Yundari Yushinta Fujaya