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All Journal Jurnal Sain Veteriner Jurnal Ilmiah Farmasi Jurnal Veteriner Jurnal Tumbuhan Obat Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Biotechnology Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Jurnal Kedokteran Biomedika Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesian Journal of Chemical Science Jurnal Farmasi Sains dan Komunitas Indonesian Journal of Chemistry JSFK (Jurnal Sains Farmasi & Klinis) Journal of Tropical Pharmacy and Chemistry JURNAL ILMU KEFARMASIAN INDONESIA JURNAL KAJIAN VETERINER Jurnal Kefarmasian Indonesia Jurnal Sains dan Kesehatan Khazanah: Jurnal Mahasiswa JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Kedokteran Hewan

Sitarina Widyarini

Departemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta

Author-ID : 382476

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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Aktivitas Antiviral Minyak Atsiri Jahe Merah terhadap Virus Flu Burung (ANTIVIRAL ACTIVITY OF ESSENSIAL OIL RED GINGER ON AVIAN INFLUENZA) Tri Untari; Sitarina Widyarini; Michael Haryadi Wibowo
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The studies have reported that ginger have many activities such as antiemesis, anti-inflammatory,anti-bacterial and anti-parasites. Therefore, this study was conducted to evaluate antiviral effect of essentialred ginger oil againts Avian Influenza (AI) in ovo using hemagglutination test (HA). Avian Influenzaviruses were treated with 0,01%, 0,1% and 1% of essential red ginger oil, and then inoculated in chickenembryonated egg via allantoic sac. Allantoic fluid was harvested using for HA test . Result of this studyshows that application of 1% of essential red ginger oil results in the reduction of titer HA . Interestingly,essential oil shows antiviral activity revealed HA titre 20 whereas the titre HA AI which AI virus treatedwith 0,01% and 0,1% essential red ginger oil, the HA titer was 25. The conclution of this study proved thatessensial oil 1% of the red gingger is the best concentration as antiviral activity .

Identifikasi Cacing Trematoda dan Gambaran Patologi Ginjal Burung Merpati yang Terinfeksi (IDENTIFICATION AND PATHOLOGICAL FEATURES OF TREMATODE IN PIGEON’S KIDNEY) Ana Sahara; Joko Prastowo; Dwi PriyoWidodo; Eryl Sri Rohayati; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study is conducted to identify species of trematode and its pathological features in pigeons’kidney. Twenty five of Yogyakarta pigeons were examined for trematode infection in kidneys. One ofkidneys was mashed in mortar with a little water, the other was examined for histopathological featuresstained with hematoxyline-eosin.Trematodes found were stained with Schmison ´s Carmine. Seven pigeons(28%) were infected by trematode with non significant clinical features. Identification trematodescharacterized by oral sucker, pharynx, testes are slighty diagonal in position, irregular in shape and intracaecal. Ovary pretesticular and vitellaria widespread from anterior ovarium to the posterior body.Histopathological examination showed segment of trematode in the medullary collecting ducts lumen,dilatation, flattening and emptyness of ducts epithelial cells, emptyness, giant cells and dominationmononuclear cell in interstitial tissue, characterizing a granulomatous nephritis. Trematodes foundidentified as Paratanaisia bragai. The trematodes were found in very dilated medullary collecting ductsand caused inflammation in adjacent tissues. Further studies are needed to find out vector of trematodein pigeons .

Terapi Ammonium Khlorida-Asam Askorbat untuk Menurunkan Tingkat Keasaman Urin dan Kristalisasi Struvit pada Kucing Urolithiasis (THERAPY AMMONIUM CHLORIDE-ASCORBIC ACID FOR LOWERING URINE ACIDITY AND STRUVITE CRYSTALLIZATION IN FELINE UROLITHIASIS) Alfarisa Nururrozi; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Hary Purnamaningsih; Sitarina Widyarini; Slamet Raharjo; Dhasia Ramandani
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Struvite/Magnesium ammonium phosphate (MAP) is common causes of feline urolithiasis. Prevent the formation of MAP crystallization can be treated by adjusting the pH urine in acid conditions. Urine with an acid pH will decrease struvite activity product (SAP) by preventing the phosphate deprotonation process which is the main constituent of struvite components. This study aims to determine the potential of a combination of ammonium chloride (NH4Cl) and ascorbic acid to decrease SAP by retain the urine pH under normal conditions. Twelve male cats, 2-5 years old and body weight 3.0 ± 0.8 kg were diagnosed struvite urolithiasis used for this study. Cats have a9clinical history of hematuria, dysuria, polyuria, and stranguria. Diagnosis of urolithiasis performed by clinical examination, USG, x-rays, and urinalysis. Cats that found struvite urolith, treated with 200 mg/kg NH4Cl and 100 mg/kg ascorbic acid orally twice a day. The research objects were observed included urine pH and crystaluria density. The data were analyzed using analisis of varian. The result of this study showed NH4Cl and ascorbic acid treatment can decrease the urine pH at 8 cats (89%) with an average pH 6.3 ± 0.3. Microscopic examination of the urine showed the struvite crystallization more infrequently than before therapy. The study concluded that the combination of NH4Cl and asam askorbat can lower the urine pH and reduce struvite crystal density in the urine.

Infeksi Microsporum canis pada Kucing Penderita Dermatitis (MICROSPORUM CANIS INFECTION IN DERMATITIS CATS) Soedarmanto Indarjulianto; Yanuartono Yanuartono; Sitarina Widyarini; Slamet Raharjo; Hary Purnamaningsih; Alfarisa Nururrozi; Nurman Haribowo; Hizriah Alief Jainudin
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Dermatitis in cats can be caused by Microsporum canis and is zoonotic. This study aims to perform clinical examination, laboratory examination, isolation, and identification of mold M. canis in cats with dermatitis. Skin scrapped from 30 cats that clinically showed lesions of dermatitis i.e. combination of alopecia, erythema, papules, pustules, scaly, and crusty were used in this study. Examination of clinical lesions and screening tests using the Wood’s lamps were performed prior to sampling. Skin scrapes samples were cultured onto Sabouraud’s dextrose agar medium, incubated at 28 ° C and colonies were identified macroscopically then stained using Lactophenol Cotton Blue for microscopic examination. Seventen of the 30 samples (56.7%) were identified as M. canis macroscopically and microscopically. The skin lesions observed in the 17 M. canis infected cats were erythema, alopecia, scaly, and crusty distributed to the ear, body, neck, back and tail of cats, respectively ABSTRAK Dermatitis pada kucing dapat disebabkan oleh Microsporum canis dan bersifat zoonotik. Penelitian ini bertujuan melakukan pemeriksaan klinis, pemeriksaan laboratoris, isolasi, dan identifikasi kapang M. canis pada kucing penderita dermatitis. Kerokan kulit dari 30 ekor kucing yang secara klinis menunjukkan lesi dermatitis berupa kombinasi dari alopesia, eritema, papula, pustula, bersisik, dan berkerak digunakan dalam penelitian ini. Pemeriksaan lesi klinis dan uji screening dengan lampu Wood’s dilakukan sebelum pengambilan sampel. Sampel kerokan kulit dikultur pada media Sabouraud’s dextrose agar, diinkubasi pada suhu 28°C dan diidentifikasi secara makroskopis. Koloni yang tumbuh diperiksa secara mikroskopis menggunakan pewarnaan Lactophenol Cotton Blue. Hasil penelitian menunjukkan 17 dari 30 sampel (56,7%) teridentifikasi M. canis secara makroskopis dan mikroskopis. Lesi kulit yang ditemukan pada 17 kucing positif terinfeksi M. canis menunjukkan adanya eritema, alopesia, bersisik, dan berkerak dengan lokasi penyebaran pada telinga, badan, leher, punggung atau ekor.

Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.

Kepadatan Sel Hipokampus Insulin Imunoreaktif pada Formasi Hipokampus Mencit yang Diinduksi Berulang dengan Streptozotosin (THE DENSITY OF HIPPOCAMPUS INSULIN IMMUNOREACTIVE CELLS IN HIPPOCAMPUS FORMATION OF REPEAT STREPTOZOSIN INDUCED MICE) Erwin .; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The presence of insulin in the hippocampus may indicate its involvement in brain cognitive function,such as learning and memory phenomena. The purpose of this study was to find out the density ofhippocampus insulin immunoreactive cells in hippocampus formation in Balb-C mice which treated withstreptozosin repeated as the animal model of diabetes mellitus. Thirty male mice Balb-C strain, aged 12-14 weeks, weight 30-40 g, divided into 2 treatment groups, each group consisted of 15 individuals. GroupI (KI) was treated with sodium citrate buffer, while group II (K2) was treated with streptozotosin at dose0,5 ml of 40 mg/kg bw in 50 mM sodium citrate buffer pH 4.5 in intra-peritoneal of for five consecutive days.Every two animals from each group euthanasia and necropsied on day 7, 14, 21 and 28 respectively afterthe administration of treatment. Subsequently, the brain tissues were collected and fixatived in NBF10%. Brain sampel were the processed immunohistochemically using anti-insulin mouse antibody. Thedensity of hippocampus insulin immunoreactive cells in hippocampus formation in group 1 were highercompared to group 2. This comparasion as well as the time of observation and interaction between groupand time showed significant differences (p<0.05). it can be concluded that low-dose induction of repeatedstreptozotosin may cause a decrease in density of hippocampus insulin imunoreaktif cell.

Jumlah dan Ukuran Nodul Tumor Kolorektal Berbanding Lurus dengan Ekspresi Siklooksigenase-2 (THE CORRELATION OF THE NUMBER AND THE SIZE OF COLORECTAL TUMOR NODULE WITH CYCLOOXYGENASE-2 EXPRESSION) Risfah Yulianty; Riska Nufika; Sitarina Widyarini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Study chemically induced colon cancer with 1,2-Dimethylhydrazine (DMH) has been widely used.DMH induction to rodentia as animal model have been similarity with colon cancer morphlogy of human.Cyclooxygenase-2 (COX-2) are important enzymes that affect carcinogenesis.COX-2can be induced byvarious stimuli, including inûammation, growth factors, and cytokines produced by tumor cells. The aim ofthis study is to investigate the correlation between the expression of COX-2 and the doses variation ofDMH, the number and the size of colorectal tumor nodule.Six male Wistar rats were randomly divided into 4 groups each group consists of six rats. Group I is control group. Group II, III, and IV were given 20, 40,and 60 mg/kg BW of DMH respectively. DMH was subcutaneously injected in the hip area, once a week for15 weeks. All animals were sacrificed at week 26th, the colon were removed and fixed in 10% bufferformalin for macroscopic and microscopic examination. The results of this study showed that there is acorrelation between various doses of DMH (P<0.05) with number of tumor nodule but not with the size ofcolorectal tumor nodule (P>0.05). Microscopic examination by using HE staining shows that inductionwith doses variation ofDMHresultin different morphololical form of tumour tissue. Induction of 20, 40,and 60 mg/kg BW DMH result in adenoma(100.00%),adenoma and adenocarcinoma (33.30% and 66.67%),and 100.00% adenocarcinoma respectively. The happens of COX-2 also correlate with in this studythatexpression of COX-2 correlate with the number and size of colorectal tumor nodule (P<0.05). However,there is no correlation between COX-2 expressionand doses variation of DMH. In conclusion, there is adose-dependent response in the number and the size of colorectal tumor nodule.

Dampak Induksi Karsinogenesis Glandula Mammae dengan 7, 12-dimetilbenz(?)antrasen terhadap Gambaran Histopatologis Lambung Tikus Sprague Dawley Ranita Tri Budi M; Sitarina Widyarini
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This experiment was designed to study the impact of mammary carcinogenesis induced by 7,12-dimetilbenz (?) antracene (DMBA) on histopathological features of the gastric in Sprague Dawley rat.Eight female Sprague Dawley (SD) rats, of four week-old, were used in this study. The rats were randomlydivided into 2 groups of four. Group I were treated orally with 20 mg/kg BW of 7,12-dimetilbenz (?)antracene (DMBA) treatment, twice a week for 5 weeks. Group II were used as negative control withoutDMBA treatment. At 5 week after the end DMBA of, the rats of the both groups were sacrificed and thegastric were fixed in 10% buffer formalin. Subsequently, tissue sections were stained with hematoxylinand eosin (H&E) for microscopic examination. The results of the experiment show that oral administrationof DMBA (20 mg/kg BW) twice a week, for 5 weeks result in epithelial hyperplasia of cardia. Hemorrhagicand inflammation reactions are also found on the mucous membrane of fundic. However, macroscopicexamination shows no specific changes of the gastric. Histopathological changes were suspected as a precancer lesion of the gastric due to chronic exposure of DMBA.

Co-Authors . Harjadi . Irianiwati . Muttaqien Abd. Rasyid Syamsuri Abdul Rohman Achmad Mursyidi Achmad Mursyidi Adrenalin, Sruti Listra Agnes Nora Iska Harnita Agnesia Endang Tri Hastuti Wahyuni Agung Endro Nugroho Agung Giri Samudra Agustina Dwi Wijayanti AGUSTINUS YUSWANTO Ahmad Mursyidi Alfarisa Nurrurozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Ana Sahara Anandita Nurwijayanti Andayana Puspitasari Gani Anna Ekawati Asmarani Kusumawati Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Deasy Wulan Dwiratna Devi Usdiana Rosyidah Devita Anggraeni Dewajani Purnomosari Dewajani Purnomosari Dhasia Ramandani, Dhasia Dhirgo Adji Dimas Adhi Pradana Dwi Priyowidodo Edy Meiyanto Eka Kumalasari Endang Dwi Wulansari Erma Yunita Erwin . Erwin E Eryl Sri Rohayati Eti Nurwening Sholikhah Etriwati E Fajar Slamet Riana Fara Azzahra Fara Azzahra FARIDA HAYATI Fauziah, Ima Frans J.M. Harren Galih Dwi Mulyati Gede Bayu Suparta Gemini Alam Geovani Meryza Oka Putra Caesar Gharsina Ghaisani Yumni Hady Anshory Hamid, Iwan Sahrial Harjadi Harjadi Hary Purnamaningsih Hary Purnamaningsih Hastari Wuryastuty Hayati, Farida Helmina Wati Hizriah Alief Jainudin Ign Edi Santosa Ika Tidariani Imanjati, Lynda Nugrahaning Indwiani Astuti Indwiani Astuti Irianiwati Irianiwati Joko Prastowo Jumina Jumina Kintoko, Kintoko Kony Putriani Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Laksono Trisnantoro Lalily Apriani Lintang Cahya Saputri Lukman Hakim Lukman Hakim Marchaban Marchaban Mega Cahya Nalasukma Michael Haryadi Wibowo Mustofa Mustofa Mustofa Mustofa Nanang Fakhrudin, Nanang Nemay Anggadewi Ndaong Neni Tri Anggraini NGATIDJAN NGATIDJAN Ni'ma, Neli Syahida Nina Salamah Noor Ardhi Pratomo Novrita Padauleng Novrita Padauleng Nura Ustrina Nurman Haribowo Pramono, Suwidjiyo Puspa Wikan Sari R Wasito R Wasito Ranita Tri Budi M Rifqi Ferry Balfas Risfah Yulianty Risfah Yulianty Riska Nufika Riska Nufika Ruslin Hadanu Rusmihayati Rusmihayati Sardjiman S Siti Salma Yusuf Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Herwiyanti Sri Herwiyanti, Sri Subagus Wahyuono Subagus Wahyuono Sudarsono Sudarsono Sudibyo Martono Sugiyanto . Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sugiyanto, Sugiyanto Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sultan Suhrah Febrina Karim Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Tri Untari Tri Untari Tri Wahyu Pangestiningsih Tria Zakinah Vembriarto Jati Pramono Vinsa Cantya Prakasita Wahyu Widiyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Widhihastuti, Endah Yanuartono . Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yeremia Yobelino Sitompul Yuli Purwandari Kristiangingrum Yuli Purwandari Kristianingrum Yustina Sri Hartini

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