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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset

Agus Purwito

Departemen Agronomi Dan Hortikultura, Institut Pertanian Bogor Jl. Meranti, Kampus IPB Dramaga, Bogor, Indonesia

Author-ID : 350191

Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Other

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Multiplikasi Tunas In Vitro Anggrek Phalaenopsis dan Analisis Keragaman Genetik dengan Marka SNAP Mira Humaira; Agus Purwito; Sudarsono; Dewi Sukma
Indonesian Journal of Agronomy Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Clonal propagation of orchid by in vitro culture is needed to obtain the high quantity and quality of orchid propagules. This study aimed to understand the effect of BAP (benzyl amino purine) concentrations on the shoot multiplication of three Phalaenopsis hybrid populations and to analyze genetic diversity using Pto-SNAP markers on clonal plantlets. The experiments were performed separately for each population, namely shoots from seed germination of Phal. amabilis x Phal. ‘KHM 421’ with five level of BAP (0.00, 11.09, 22.19, 33.29, 44.39 µM), clonal shoots from Phal. ‘Salu Spot’ x Phal. bellina ‘1102-38’, and Phal. ‘Salu Spot’ x Phal. bellina ‘1102-44’ with four level of BAP (11.09, 22.19, 33.29, 44.39 µM). The results showed that the optimum BAP concentration for shoot multiplication on three population was 22.19 µM which shoot number obtained were 3.7, 4.7, and 6.0 at 12 week after planting (WAP). Protocorm like bodies (PLBs) was only produced on Phal. amabilis x Phal. ‘KHM 421’ population, which the highest number of PLBs was on 11.09 BAP. Evaluation of genetic diversity using 11 Pto-SNAP (single nucleotide amplified polymorphism) marker was performed on Phal. ‘Salu Spot’ x Phal. bellina ‘1102-44’ shoots. The results revealed the presence of somaclonal variation with 7.7% of genetic diversity of the shoots from BAP 22.19 µM treatment at Pto-241 and 11.1% from BAP 44.39 µM treatment at Pto-424. Keywords: clonal propagation, cytokinin, molecular markers, orchid, Pto, somaclonal variation

Embriogenesis Somatik dari Eksplan Daun Anggrek Phalaenopsis sp L. (Somatic Embryogenesis from Leaf Eksplant of Phalaenopsis Orchids) Sri Rianawati; Agus Purwito; Ridho Kurniati; Budi Marwoto; Suryanah Suryanah
Indonesian Journal of Agronomy Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Somatic embryogenesis has been recoqnized as one of the process on plant micropropagation techniques. This process occured through regeneration by direct embryo formation and through an intermediary callus phase. This research was conducted through an intermediary callus phase. The experiment was initiated with callus induction from leaf explant on five modifications of MS medium i.e :1/2MS without plant hormone (MI-0); ½ MS containing 1mg/L BA + 0.5 mg/L 2.4-D + 1mg/L NAA (MI-1);1/3 MS containing 2 mg/L 2.4-D (MI-2); ½ MS supplemented with 0.5 mg/L 2.4-D + 0.5 mg/L BAP +0.2 mg/L thidiazuron (MI-3); ½ MS supplemented 2 mg/L thidiazuron and 1 mg/L BAP (MI-4). After the tissues were swollen, the explants were placed on callus proliferation medium ½ MS supplemented with 0.2 mg/L thidiazuron and 0.5 mg/L 2.4-D (MP). After two months, calli were regenerated in regeneration medium ½ MS supplemented with 0.4 mg/L BAP and 0.2 mg/L 2.4-D (MR). The results of this research showed that MI-1 and MI-3 were the best swelling explant mediums before the callus produced in both MP and MR medium. Callus produced was increased in every subculture. However, the level of callii production decreased on the following subculture. Plantlets were regenerated from somatic embryos derived from callii on MR medium. The results of this study may contribute to our advancement of scientific knowledge achievements tissue culture techniques to support inconventional plant improvement. Key words: embryo somatic induction, in vitro, embryogenic callii

Pembentukan Tanaman Cabai Haploid Melalui Induksi Ginogenesis dengan Menggunakan Serbuk Sari yang Diradiasi Sinar Gamma , Suharsono; Muhammad Alwi; Agus Purwito
Indonesian Journal of Agronomy Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this research was to obtain haploid hot pepper plants by inducing gynogenesis with g-ray irradiated pollen for pollination. Fruits resulted from pollination by irradiated pollen was used as the explants of the in vitro culture in solid MS medium containing BAP, IAA and GA3. The resulted plants then cultivated in MS0 medium. The result of the research showed that hot pepper immature embryo could grow and developed into whole plant when the age of this embryo was 9 days after pollination or more. The haploid hot pepper plants can be obtained by pollinating the pistil with 10 Gy irradiated pollen. The MS medium containing BAP 0.3 + IAA 0.2 + GA3 0.5 mg/l and BAP 0.4 + IAA 0.1 + GA3 0.5 mg/l supported well the development of immature embryo into whole plants. The growth of haploid hot pepper plants was slower than that of diploid ones. Key words: Hot pepper, haploid plant, gynogenesis, g-ray.

Studi RegeDerasi daD Produksi Protoplas Mesofil Daun Beberapa KlOD TanamaD Kentang (Solanum tuberosum L.) . , Asnawatr; G. A. Wattimenal; M. Machmud; Agus Purwito
Indonesian Journal of Agronomy Vol. 30 No. 3 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objectives of these experiments were to obtain medium composition to enlarge leaf size for protoplast production and to obtain medium composition for plant regeneration. The result showed that the best medium to produce the larger leaves was medium MS with double concentration of macronutrients without hormone supplemented with Morel vitamins, 3% (w/v) sucrose and. 7 g/l agar. This medium produced leaves with diameter of 1.44 cm comparing to control medium MS with 0.67 cm in diameter. Medium MS containing 0.1 mg/l 1AA, 0.5 mg/l Zeatin and 0.5 mg/l GA3 was able to regenerate vigorous shoots of 7 clones. Protoplast isolation of 5 clones using enzyme composition containing 0.5 % cellulase Onozuka RS, 0.05 %, pectolyase Y-23, 0.05 % MES, 9.1 % mannitol and pH 5.5, without CP W medium produced protoplast with variable yield from 10.50 x 1oJ protoplast/g leaves for Atlantic to 46.58x 1oJ protoplast/gforBF15. Keyword: Leaves size, Plant regeneration, Potato, Protoplast i

Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri , Nurhasanah; G. A. Wattimena; Agus Purwito; Ni Made Armini Wiendi; , Suharsono
Indonesian Journal of Agronomy Vol. 31 No. 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Hordothionins are small anti-bacteria proteins present in barley endosperm, To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Agrobacterium tumefaciens strain LBA 4404, under the control of a cauliflower mosaic virus (CaMV) 358 promotor. The in vitro grown stem (internodus) was used in this research. After 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesific primer from CaMV 358 promotor showed the presence of amplified T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested, The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level, There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. Key words: Potato, Hordothionin gene, Disease resistance

Perbanyakan Tunas Mikro Pisang Rajabulu (Musa AAB Group) dengan Eksplan Anakan dan Jantung Andri Ernawati; Agus Purwito; J. M. Pasaribu
Indonesian Journal of Agronomy Vol. 33 No. 2 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Research on micropropagation of banana cv. Rajabulu (Musa AAB Group) was undertaken. On initiation stage, sucker was best used as explant during rainy season on solid medium containing 7 mg/l BAP + 3 mg/l IAA and average microshoots was 7 shoots/bottle. While inflorescence as explant was best for initiation during dry season on solid medium containing 9 mg/l BAP + 1 mg/l IAA and average microshoots was 18 shoots/bottle. On multiplication stage, sucker produced microshoot on medium containing 4.5 mg/l BAP + 1.0 mg/l kinetin (2.9 shoots). While inflorescense produced microshoot on medium containing 6.0 mg/l IAA + 2.0 mg/l BAP (2.4 shoots). Acclimatization of plantlets produced from sucker and inflorescense was best on medium of compost 75% + soil 25%. Key words: micropropagation, sucker, inflorescense, banana Rajabulu (Musa AAB Group)

Perbanyakan Ruskus (Ruscus hypophyllum L.) secara In Vitro Agus Purwito; Prima Muklisa; Awang Maharijaya
Indonesian Journal of Agronomy Vol. 33 No. 2 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

These experiments were aimed to obtain optimum medium for micropropagation of Ruscus. There were two experiments consist of in vitro shoots proliferation, shoot elongation and rooting. The experiment of shoot proliferation performed by inducing adventitious shoots from explant in the Murashige and Skoog (1962)(MS) basal medium supplemented with combination of plant growth regulators BAP (0.0, 0.5, 1.0, 2.0, 4.0 and 6.0 mg/l) and IAA (0.0, 0.1, 0.2 and 0.4 mg/l). The elongation and rooting of plantlets were induced in the different concentration of the MS basal medium (0.5, 1.0 and 2.0 strength) combined with IBA (0.0, 1.0, 2.0, 3.0 and 4.0 mg/l). Both experiments were arranged as completely randomized design with 15 replications. Adventitious shoots were produced in all medium supplemented with BAP with or without IAA. However MS medium supplemented with BAP 1 mg/l or 2 mg/l combined with IAA 0.2 mg/l were the best. The number of adventitious shoots in these medium were 9.2 and 9.4 shoots after 8 weeks cultured respectively. Increasing concentration of BAP more than 4 mg/l decreased number and size of adventitious shoots. The plantlets produced in the proliferation medium were then transferring to the next treatments for elongation and rooting. The best medium for elongation and rooting were medium with half strength of MS with or without IBA. Acclimatization conducted by transferring the rooted plantlets on the medium containing sterilized soil and rice husk charcoal (1:1). After 4 weeks acclimatization, 60-100 percent of plantlets were survived and growth, depend on treatments. Key words: Acclimatization, adventitious shoot, micropropagation, rooting, Ruscus hypophyllum L.

Perakitan Kultivar Kentang Unggul Indonesia secara Cepat dengan Metode Turunan Klonal Biji Tunggal dan Pra - Evaluasi Secara In Vitro G. A. Wattimena; Agus Purwito; H . M. Machmud; , Samanhudi
Indonesian Journal of Agronomy Vol. 29 No. 3 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

At least ten years needed to abtain new potato cultivar through sexual hybridization, somatic hybridization or through genetic transformation, To short cut this process, Laboratory of Biotechnology, Department of Agronomy, IPB employed a strategy so called single seed in vitro clonal decent ( SSICD) by usings elected parental lines for TPS (True Potato Seed) production. This breeding consist of in vitro pre evaluation for resistance wilt, fusarium wilt, black leg, rot knot nematode and maturity. Using the same number of bacterial cell / (109 cell/ml), there were positive correlation between in vitro test for disease resistance through dripping test or dripping test with greenhouse test through direct inoculation of Ralstonia solanancearum. Resistant clones to fusarium wilt and verticillium were also resistant to bacterial wilt. In vitro tuberization could be use to evaluate maturity of potato cultivar. Key words: Potato, SSICD

ISOLASI DAN REGENERASI PROTOPLAS DARI MESOFIL DAUN KENT ANG (Solanum tuberosum L) DIHAPLOID Agus Purwito; G. A. Wattimena; Antonius Suwanto; Inez H.S. Loeddin Suharsono; Hajrial Aswidinnoor
Indonesian Journal of Agronomy Vol. 27 No. 1 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid cultivars of potato BF 15 and SVP 10 leaves used four different enzymes solution. Protoplast were cultured onto four different cultures media to increase plating efficiency. Calli were then transferred to ten different regeneration media. Using cellulose RS 0.5 % and pectolyase Y-23 0.05 % protoplast yield of both cultivars were improved. Medium VKM supplemented with 0.2 mg/l 2, 4-D, 1.0 mg/l NAA and 0.5 mg/l zeatin or 2iP were increase recovery of colonies from protoplast up to 5.9%. Regeneration medium containing zeatin did always produce more shoots than those of 2iP. Genotypes dependant regeneration frequencies have also been showed in this experiments

Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Indonesian Journal of Agronomy Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid

Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Anas D Susila Andri Ernawati Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atika Fathur Rahmi Atra Romeida Awang Maharijaya Bambang S. Purwoko Budi Marwoto Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Catur Herison dan Budi Winarto dan Sudarsono Dewi Citra Sari Dewi Sukma Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Iis Rahmawati Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Julius D. Nugroho Kartiman, Roni Kartiman, Roni Karyanti , Kikin H Mutaqin Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Marlin MASKROMO, ISMAIL MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi Muhammad Herman MUHAMMAD HERMAN Muhammad Mahmud MUHAMMAD SYUKUR Ni Made Armini Wiendi Nidya Ravenska Noor Farid Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie NURUL KHUMAIDA Nurwita Dewi Prima Muklisa Purba, Dumaris Priskila RAGAPADMI PURNAMANINGSIH Rd. Selvy Handayani Rd. Zainal Frihadian Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah ROEDHY POERWANTO Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri RR. Sri Hartati Rr. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw Sari, Laela Sientje Mandang Sumaraw SIENTJE MANDANG SUMARAW SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO SUDIRMAN YAHYA Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syarifah Iis Aisyah Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Willy Bayuardi Suwarno Wulandari, Dyah Retno Yopi Kurniawan

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