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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset
Agus Purwito
Departemen Agronomi Dan Hortikultura, Institut Pertanian Bogor Jl. Meranti, Kampus IPB Dramaga, Bogor, Indonesia
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Journal : AGRIVITA, Journal of Agricultural Science

 1 
ANALYSIS OF COMBINING ABILITY, HETEROSIS EFFECT AND HERITABILITY ESTIMATE OF YIELD-RELATED CHARACTERS IN SHALLOT (Alium cepa var. ascalonicum Baker) Farid, Noor; Sugiharto, Arifin Noor; Herison, Catur; Purwito, Agus; Sutjahjo, Surjono Hadi
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Low productivity of shallot in Indonesia can be improved through development of high yielding variety. The availability of genetic information related to the character being improved is inevitable for effective breeding program. In this study, seven shallot genotypes were evaluated for their combining ability for yield improvement using half diallel cross.  Heterosis effect and heritability estimate was also investigated for yield-related characters.  The results showed that there were significant differences in general combining ability (GCA) and specific combining ability (SCA) among the evaluated genotypes. Tiron and Timor had the greatest GCA.  The greatest SCA and heterosis value was found in crosses of Kuning/Tiron, Timor/Bima Juna, Tiron/Timor and Kuning/Sibolangit. Heterosis effect varied from low to high. Broad sense heritability estimate for all characters was high, but narrow sense heritability was low for most charaters.  The dominant gene action observed on all yield-related characters suggests that the evaluated genotypes are potential to be used in breeding for high yielding hybrid varieties.   Keywords: GCA, SCA, heterosis, heritability, shallot
ANALYSIS OF COMBINING ABILITY, HETEROSIS EFFECT AND HERITABILITY ESTIMATE OF YIELD-RELATED CHARACTERS IN SHALLOT (Alium cepa var. ascalonicum Baker) Farid, Noor; Sugiharto, Arifin Noor; Herison, Catur; Purwito, Agus; Sutjahjo, Surjono Hadi
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i1.142

Abstract

Low productivity of shallot in Indonesia can be improved through development of high yielding variety. The availability of genetic information related to the character being improved is inevitable for effective breeding program. In this study, seven shallot genotypes were evaluated for their combining ability for yield improvement using half diallel cross.  Heterosis effect and heritability estimate was also investigated for yield-related characters.  The results showed that there were significant differences in general combining ability (GCA) and specific combining ability (SCA) among the evaluated genotypes. Tiron and Timor had the greatest GCA.  The greatest SCA and heterosis value was found in crosses of Kuning/Tiron, Timor/Bima Juna, Tiron/Timor and Kuning/Sibolangit. Heterosis effect varied from low to high. Broad sense heritability estimate for all characters was high, but narrow sense heritability was low for most charaters.  The dominant gene action observed on all yield-related characters suggests that the evaluated genotypes are potential to be used in breeding for high yielding hybrid varieties.   Keywords: GCA, SCA, heterosis, heritability, shallot
Effect of Gamma Rays Irradiation and In Vitro Selection on Citrus nobilis (L.) ‘Siam Banyuwangi’ to Huanglongbing (HLB) Disease Dumaris Priskila Purba; Ali Husni; Alina Akhidaya; Mia Kosmiatin; Agus Purwito
AGRIVITA, Journal of Agricultural Science Vol 43, No 2 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i2.2887

Abstract

Siam orange (Citrus nobilis L.) provides great economic value and social importance, despite its susceptibility to Huanglongbing (HLB) disease caused by Ca. Liberabacter sp., classified as specific bacterium phloem vessels marked by the formation of callouses covering plasmodesmata. This research aims to improve the tolerance of Siam orange from Banyuwangi (SB) to HLB disease. The experiment was undertaken by performing a randomized design (CRD) with one factor (irradiation dose). This study consists of two interrelated experiments, which include: induction of embryo mutations with gamma rays irradiation, and in vitro selection of putative mutant. The embryo of SB was irradiated by gamma rays with doses of 0, 45, 50, and 55 Gray. Each treatment was repeatedly undertaken for five times with 20 embryos. After 24 weeks, in vitro selection of putative mutant shoots was screened by HLB pathogen suspension. The observation indicated that treatment of gamma rays in various doses influenced embryo germination. In general, gamma ray irradiation gave significant effects on embryo germination and plant morphological characters. In vitro selection results for putative mutant on doses of 45, 50, and 55 indicated tolerance to HLB pathogen after selection.
Protoplast Fusion between Indonesian Citrus maxima (Burm.) Merr. and Citrus reticulata L.: A Preliminary Report Dyah Retno Wulandari; Agus Purwito; Slamet Susanto; Ali Husni; Tri Muji Ermayanti
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.950

Abstract

Protoplast fusion is a useful technique for citrus genetic improvement. Establishment of protoplast technology could produce triploid seedles citrus and new citrus genetic combination. The aim of this research was to establish protoplast isolation, protoplast fusion between pummelo ‘Nambangan’ (Citrus maxima) and keprok ‘Garut’ (C. reticulata), and its protoplast culture. Protoplasts were isolated from leaves of in vitro seedlings of pummelo ‘Nambangan’ and embryogenic callus of keprok ‘Garut’ with two different compositions of enzymes. The results showed that optimum enzymes compositions for cells mesophyl wall degradation were consisted of 0.5 % Onozuka cellulase RS10, 0.5 % Macerozyme R10 and 0.1 % Pectolyase Y23. Optimum enzymes composition for callus embryogenic cells wall degradation were 0.5 % Onozuka cellulase RS10 added with 0.5 % Macerozyme R10. Protoplast fusion was conducted with PEG-mediated. Protoplast mixture were incubated in 40 % PEG for 8-10 min and after that could be observed 40.6 % of unfused protoplasts, 4.6 % of homofused mesophyl protoplasts, 28.2 % of homofused callus protoplasts, 7.8 % of heterofused and 9.6 % of multifused protoplasts from mesophyl and callus. Cells divided at the first week and began to form cell colonies afterwards in liquid culture.
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Anas D Susila Andri Ernawati Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atika Fathur Rahmi Atra Romeida Awang Maharijaya Bambang S. Purwoko Budi Marwoto Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Catur Herison dan Budi Winarto dan Sudarsono Dewi Citra Sari Dewi Sukma Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Iis Rahmawati Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Julius D. Nugroho Kartiman, Roni Kartiman, Roni Karyanti , Kikin H Mutaqin Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Marlin MASKROMO, ISMAIL MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi Muhammad Herman MUHAMMAD HERMAN Muhammad Mahmud MUHAMMAD SYUKUR Ni Made Armini Wiendi Nidya Ravenska Noor Farid Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie NURUL KHUMAIDA Nurwita Dewi Prima Muklisa Purba, Dumaris Priskila RAGAPADMI PURNAMANINGSIH Rd. Selvy Handayani Rd. Zainal Frihadian Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah ROEDHY POERWANTO Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri RR. Sri Hartati Rr. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw Sari, Laela Sientje Mandang Sumaraw SIENTJE MANDANG SUMARAW SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO SUDIRMAN YAHYA Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syarifah Iis Aisyah Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Willy Bayuardi Suwarno Wulandari, Dyah Retno Yopi Kurniawan