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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset
Agus Purwito
Departemen Agronomi Dan Hortikultura, Institut Pertanian Bogor Jl. Meranti, Kampus IPB Dramaga, Bogor, Indonesia
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Journal : JURNAL BIOLOGI INDONESIA

 1 
Kajian Pendahuluan: Perpindahan Gen dari Tanaman Kentang Transgenik Katahdin RB ke Tanaman Kentang Non Transgenik Ambarwati, A. Dinar; Herman, M.; Purwito, Agus rifcb@indo.net.id; Sofiari, Eri; Aswidinnoor, hajrial
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3114

Abstract

ABSTRACTPreliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenicpotato. One of the concerns associated with the release of transgenic crops, is the possibilityof the gene flow from transgenic crops to neighboring crops of the same species or to relatedspecies. In plants, gene flow is a routine process occur through the natural hybridization. Theopportunity for gene flow occur depends principally on two factors, the degree of sexualcompatibility between donor and recipient species, and the physical distance between thetwo. The experiment was conducted to determine whether the gene flow from transgenicpotato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/lkanamycin, and to estimate gene flow mediated by natural hybridization at different isolationdistances. Preliminary result indicated that a rapid and simple method using MS0 liquid mediawith kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow fromtransgenic potato Katahdin RB to non transgenic, based on a rapid and simple selectionmethod using 50 mg/l of kanamycin as selectable marker. The isolation distance used in thestudy were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row oftransgenic potato Katahdin RB. The gene flow through natural hybridization at a isolationdistances of (0.8 - 1.6 m), (2.4 – 4 m), and (4.8 – 6.4 m) from transgenic to non transgenic plantswere 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 – 8 m, the frequency of gene flowwas declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenicpotatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.Key words : natural hybridization, transgenic potato RB, kanamycin selection
KAJIAN PENDAHULUAN: PERPINDAHAN GEN DARI TANAMAN KENTANG TRANSGENIK KATAHDIN RB KE TANAMAN KENTANG NON TRANSGENIK Ambarwati, A. Dinar; Herman, M.; Purwito, Agus rifcb@indo.net.id; Sofiari, Eri; Aswidinnoor, hajrial
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3114

Abstract

ABSTRACTPreliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenicpotato. One of the concerns associated with the release of transgenic crops, is the possibilityof the gene flow from transgenic crops to neighboring crops of the same species or to relatedspecies. In plants, gene flow is a routine process occur through the natural hybridization. Theopportunity for gene flow occur depends principally on two factors, the degree of sexualcompatibility between donor and recipient species, and the physical distance between thetwo. The experiment was conducted to determine whether the gene flow from transgenicpotato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/lkanamycin, and to estimate gene flow mediated by natural hybridization at different isolationdistances. Preliminary result indicated that a rapid and simple method using MS0 liquid mediawith kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow fromtransgenic potato Katahdin RB to non transgenic, based on a rapid and simple selectionmethod using 50 mg/l of kanamycin as selectable marker. The isolation distance used in thestudy were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row oftransgenic potato Katahdin RB. The gene flow through natural hybridization at a isolationdistances of (0.8 - 1.6 m), (2.4 ? 4 m), and (4.8 ? 6.4 m) from transgenic to non transgenic plantswere 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 ? 8 m, the frequency of gene flowwas declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenicpotatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.Key words : natural hybridization, transgenic potato RB, kanamycin selection
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Anas D Susila Andri Ernawati Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atika Fathur Rahmi Atra Romeida Awang Maharijaya Bambang S. Purwoko Budi Marwoto Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Catur Herison dan Budi Winarto dan Sudarsono Dewi Citra Sari Dewi Sukma Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Iis Rahmawati Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Julius D. Nugroho Kartiman, Roni Kartiman, Roni Karyanti , Kikin H Mutaqin Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Marlin MASKROMO, ISMAIL MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi Muhammad Herman MUHAMMAD HERMAN Muhammad Mahmud MUHAMMAD SYUKUR Ni Made Armini Wiendi Nidya Ravenska Noor Farid Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie NURUL KHUMAIDA Nurwita Dewi Prima Muklisa Purba, Dumaris Priskila RAGAPADMI PURNAMANINGSIH Rd. Selvy Handayani Rd. Zainal Frihadian Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah ROEDHY POERWANTO Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri RR. Sri Hartati Rr. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw Sari, Laela Sientje Mandang Sumaraw SIENTJE MANDANG SUMARAW SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO SUDIRMAN YAHYA Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syarifah Iis Aisyah Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Willy Bayuardi Suwarno Wulandari, Dyah Retno Yopi Kurniawan